We narrowed to 166,911 results for: DES

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pDestattB (pCM268)

Plasmid

#68313

Purpose

Multisite Gateway destination vector to create transgenes for phiC31-mediated transgenesis in zebrafish

Depositor

Christian Mosimann, Leonard Zon

Article

Mosimann et al Dev Dyn. 2013 Aug;242(8):949-63. doi: 10

Type

Empty backbone

Use

Zebrafish transgenesis

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pDEST26-USP47

Plasmid

#64326

Purpose

Mammalian expression and purification of USP47.

Depositor

Grigory Dianov

Article

Parsons et al Mol Cell. 2011 Mar 4;41(5):609-15. doi:

Insert

USP47 (USP47 Human)

Tags

His

Expression

Mammalian

Promoter

CMV

Available Since

June 25, 2015

Availability

Academic Institutions and Nonprofits only

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pDEST14-DogCatcher

Plasmid

#171772

Purpose

Expresses DogCatcher protein in bacterial cytoplasm

Depositor

Mark Howarth

Article

Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

Insert

DogCatcher

Tags

His6 and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

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HA-GFP1-10-pDEST-N

Plasmid

#118368

Purpose

Bimolecular Fluorescence Complimentation (BiFC) assay. Gateway compatible N-terminal vector with BiFC-tag (HA-GFP1-10) to screen interaction partners.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Type

Empty backbone

Use

Destination

Tags

HA-GFP1-10

Expression

Mammalian

Available Since

May 1, 2019

Availability

Academic Institutions and Nonprofits only

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pDestTol2pA2-U6:gRNA

Plasmid

#63157

Purpose

Destination vector for Gateway based on vector #394 from the Tol2Kit, containing a gRNA scaffold under the control of a zebrafish U6 promoter.

Depositor

Leonard Zon

Article

Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15

Type

Empty backbone

Use

CRISPR; Tol2 destination vector for gateway

Available Since

April 13, 2015

Availability

Academic Institutions and Nonprofits only

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2Flag-pDEST-N

Plasmid

#118371

Purpose

Gateway compatible mammalian expression vector with N-terminal 2Flag to tag proteins of interest.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Type

Empty backbone

Use

Destination

Tags

2Flag

Expression

Mammalian

Available Since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pDEST14-SpyCatcher

Plasmid

#35044

Depositor

Mark Howarth

Article

Zakeri et al Proc Natl Acad Sci U S A. 2012 Mar 20;10

Insert

SpyCatcher

Tags

His6

Expression

Bacterial

Mutation

Deletion of C-terminal till I113, and point mutat…

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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pDest-Sumo

Plasmid

#106980

Purpose

LR reaction to create expression plasmid. For prokaryotic expression of target protein as N-terminal fusion form with Sumo tag

Depositor

Han Choe

Article

Nguyen et al Sci Rep. 2017 Nov 23;7(1):16139. doi: 10

Type

Empty backbone

Tags

His8-Sumo

Expression

Bacterial

Promoter

T7

Available Since

March 30, 2018

Availability

Academic Institutions and Nonprofits only

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1457_pDEST_miniTol2_R4-R2_Cryst_mCherry

Plasmid

#171796

Purpose

pDEST miniTol2-clone for R4-R2 3-component gateway assembly (p5E + pME). Include a mCherry selection marker (Crystallin promoter Lens/Eyes)

Depositor

Jean Giacomotto

Article

Tromp et al Front Physiol. 2023 Aug 2;14:1221310. do

Type

Empty backbone

Expression

Bacterial

Available Since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST17-UnaG

Plasmid

#203485

Purpose

For recombinant protein production in Escherichia coli. His-tagged UnaG under the control of the T7 promoter.

Depositor

Yutaka Kodama

Article

Ishikawa et al Sci Adv. 2023 Jun 9;9(23):eadh4787. doi:

Insert

UnaG

Expression

Bacterial and Plant

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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HA-GFP1-10-pDEST-C

Plasmid

#118369

Purpose

Bimolecular Fluorescence Complimentation (BiFC) assay. Gateway compatible C-terminal vector with BiFC-tag (HA-GFP1-10) to screen interaction partners.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Type

Empty backbone

Use

Destination

Tags

HA-GFP1-10

Expression

Mammalian

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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mCherry-Desmocollin1-N-18

Plasmid

#55028

Purpose

Localization: Cadherin, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

Desmocollin1 (DSC1 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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2HA-pDEST-N

Plasmid

#118373

Purpose

Gateway compatible mammalian expression vector with N-terminal 2HA to tag proteins of interest.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Type

Empty backbone

Use

Destination

Tags

2HA

Expression

Mammalian

Available Since

May 1, 2019

Availability

Academic Institutions and Nonprofits only

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pXN21-Dest

Plasmid

#105086

Purpose

Yeast mating-based Split Ubiquitin Assay, prey vector for Gateway cloning

Depositor

Christopher Grefen

Article

Grefen et al Curr Protoc Neurosci. 2007 Oct;Chapter 5

Type

Empty backbone

Tags

NubG

Expression

Yeast

Available Since

Feb. 26, 2018

Availability

Academic Institutions and Nonprofits only

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mCherry-Desmoglein1-N-18

Plasmid

#55030

Purpose

Localization: Desmosomes, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

Desmoglein1 (DSG1 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pDEST14-SpyCatcher002

Plasmid

#102827

Purpose

Bacterial expression of SpyCatcher002 for covalent coupling to SpyTag002-linked proteins

Depositor

Mark Howarth

Article

Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

Insert

SpyCatcher002

Tags

GSSGS, His6, and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

Dec. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pRosa26-DEST

Plasmid

#21189

Purpose

Rosa26 knock-in vector for Cre-regulated cDNA expression and RNAi in transgenic mice

Depositor

Nick Hastie, Peter Hohenstein

Article

Hohenstein et al Pathogenetics. 2008 . 1(1):3.

Insert

GateWay conversion cassette

Expression

Mammalian

Available Since

Sept. 2, 2009

Availability

Academic Institutions and Nonprofits only

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PB-SAM-Dest

Plasmid

#102563

Purpose

Piggybac transposon DEST vector encoding dCAS9-VP64 and MS2-P65-HSF1 activator helper complex. It is used for insertion of multiple U6-sgRNA expression cassettes.

Depositor

Ying Liu

Article

Li et al Mol Ther Nucleic Acids. 2017 Sep 15;8:64

Type

Empty backbone

Use

CRISPR; Piggybac transposon

Expression

Mammalian

Promoter

N/A

Available Since

Jan. 5, 2018

Availability

Academic Institutions and Nonprofits only

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SYNJ1-170_pCSdest

Plasmid

#53822

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

SYNJ1-170 (SYNJ1 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
SYNJ1-145_pCSdest

Plasmid

#53810

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

SYNJ1-145 (SYNJ1 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits