We narrowed to 6,508 results for: nls

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SBE-NLS-mCherry PGK-rtTA3

Plasmid

#195332

Purpose

Lentiviral construct of TGFβ reporter (nuclear mCherry) and hPGK driven rtTA3

Depositor

Elaine Fuchs

Article

Yuan et al Nature. 2022 Nov 30. doi: 10.1038/s41586

Inserts

NLS-mCherry
rtTA3

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

hPGK and miniCMV

Available Since

Feb. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-NLS-AcrIIA4

Plasmid

#113038

Purpose

AAV vector for expression of SV40NLS-AcrIIA4 in mammalian cells (CMV promoter driven)

Depositor

Dominik Niopek

Article

Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

Insert

SV40 NLS-AcrIIA4

Use

AAV, CRISPR, and Synthetic Biology

Expression

Mammalian

Available Since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pJM592 EF1α 3xFLAG-NLS-DsDed-ZF10-intN-VP64 in TUPV2

Plasmid

#161568

Purpose

Constitutive expression of 3xFLAG-NLS-DsDed-ZF10-intN-VP64 under the EF1α promoter

Depositor

Joshua Leonard

Article

Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

Insert

3xFLAG-NLS-DsDed-ZF10-intN-VP64

Use

Synthetic Biology

Tags

3x-FLAG

Expression

Mammalian

Promoter

EF1α

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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PX458-3xHA-SpCas9

Plasmid

#130968

Purpose

3xHA tagged Cas9 from S. pyogenes with T2A-EGFP and cloning backbone for sgRNA

Depositor

Debojyoti Chakraborty

Article

Acharya et al Proc Natl Acad Sci U S A. 2019 Oct 15;11

Insert

3xHA-NLS-SpCas9

Use

CRISPR

Tags

3x HA, EGFP, and NLS

Expression

Mammalian

Promoter

Cbh

Available Since

Nov. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pHR-BFP-NLS-P2A-RSV P-1xSunTag@72AA

Plasmid

#246863

Purpose

Express BFP-NLS-P2A-RSV P-1xSunTag@72AA (Pexo-SunTag) for RSV imaging.

Depositor

Marvin Tanenbaum

Article

Ratnayake et al Nature. 2026 Jan 28. doi: 10.1038/s41586

Insert

BFP-NLS-RSV P-1xSunTag@72AA

Use

Lentiviral

Tags

BFP

Expression

Mammalian

Mutation

Inserted a single SunTag copy at amino acid 72 of…

Promoter

SFFV

Available Since

Jan. 29, 2026

Availability

Academic Institutions and Nonprofits only

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AiP1181-Ai224 (TICL-nls-EGFP-ICF-nls-dTomato)

Plasmid

#181760

Purpose

targeting vector

Depositor

Tanya Daigle

Insert

nls-tagged EGFP and nls-tagged dTomato

Expression

Mammalian

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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Frb-NLS-mCherry

Plasmid

#217613

Purpose

part B of SPARK-OFF

Depositor

Xiaokun Shu

Article

Chung et al Proc Natl Acad Sci U S A. 2024 Feb 13;12

Insert

Frb-NLS-mCherry

Use

Lentiviral

Expression

Mammalian

Available Since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

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TwinStrep-SUMO-NLS-BhCas12b_v4

Plasmid

#158615

Purpose

For protein expression of BhCas12b_v4

Depositor

Feng Zhang

Article

Strecker et al Nat Commun. 2019 Jan 22;10(1):212. doi:

Insert

NLS-BhCas12b_v4

Tags

6xHIS-TwinStrep-SUMO

Expression

Bacterial

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV5 Flag-NLS-Smad2

Plasmid

#14931

Depositor

Joan Massague

Article

Lo et al Nat Cell Biol. 1999 Dec . 1(8):472-8.

Insert

NLS-Smad2 (SMAD2 Human)

Tags

Flag

Expression

Mammalian

Mutation

full-length Smad2 is fused to a nuclear-localizat…

Available Since

May 17, 2007

Availability

Academic Institutions and Nonprofits only

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FRP2371

Plasmid

#127577

Purpose

Repressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, His3MX selection marker

Depositor

Joerg Stelling

Article

Azizoglu et al Elife. 2021 Aug 3;10. pii: 69549. doi: 1

Insert

PRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1

Use

Synthetic Biology; Shuttle vector

Expression

Yeast

Available Since

Aug. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pST1374-scFv-APOBEC-UGI-GB1

Plasmid

#113029

Purpose

Expresses scFv-rAPOBEC1-UGI in mammalian cells

Depositor

Xingxu Huang

Article

Jiang et al Cell Res. 2018 Jun 6. pii: 10.1038/s4142

Insert

scFv-rAPOBEC1-UGI-GB1

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pFTK056

Plasmid

#171328

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

SpCas9-NLS

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBFv-nosP-Cas9

Plasmid

#138402

Purpose

Expresses Cas9 in the germline of Drosophila

Depositor

Shu Kondo

Article

Kondo et al Genetics. 2013 Nov;195(3):715-21. doi: 1

Insert

Cas9-NLS

Expression

Insect

Promoter

UAS

Available Since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

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BII-STAR-tdT

Plasmid

#133396

Purpose

Piggybac vector for ASCL2 reporter

Depositor

Blair Madison

Insert

tdTomato-NLS

Expression

Mammalian

Mutation

N/A

Promoter

Stem Cell Specific ASCL2 response elements with L…

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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BII-ChPtinGW

Plasmid

#133377

Purpose

Piggybac vector for constitutive expression

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Type

Empty backbone

Tags

Flag

Expression

Mammalian

Promoter

CMV enhancer and hEf1a (CpG free)

Available Since

Jan. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA-NES-DEVD-mCardinal-NLS

Plasmid

#164052

Purpose

Fluorescent reporter for caspase-3

Depositor

Robert Campbell

Article

Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

Insert

NES-mCardinal-NLS

Tags

kozak sequence

Expression

Mammalian

Promoter

CMV promotor

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pEvol-pAcFRS.2.t1

Plasmid

#73544

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of several non-standard amino acids, into proteins in E coli in response to the amber codon, TAG

Depositor

Farren Isaacs

Article

Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

Inserts

pAcFRS.2.t1
pAcFRS.2.t1

Expression

Bacterial

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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pRO280

Plasmid

#233846

Purpose

MoClo-YTK; ConL1/R2 cassette; ABA systems; pTDH3-NLS-VP48-2xOaf1-ABI-tENO1

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

pTDH3-NLS-VP48-2xOaf1-ABI-tENO1

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pWN059

Plasmid

#233853

Purpose

MoClo-YTK; ConL1/R2 cassette; Auxin systems; pTDH3-NLS-VP48-2XOaf1-AIDΔ34-tENO1

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

pTDH3-NLS-VP48-2XOaf1-AIDΔ34-tENO1

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pWN061

Plasmid

#233854

Purpose

MoClo-YTK; ConL1/R2 cassette; NS3 systems; pTDH3-NLS-VP48-2XOaf1-DNCR2-tENO1

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

pTDH3-NLS-VP48-2XOaf1-DNCR2-tENO1

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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