We narrowed to 13,520 results for: plasmids 101
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Plasmid#207362PurposeExpression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variantDepositorInsertB-HiFi SpCas9
UseCRISPRTags3xFLAGExpressionMammalianMutationR691A, amino acids 1005-1013 replaced with two gl…PromoterCBhAvailable SinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFastBac_Dual_GST-TEV-EGFP-TBK1
Plasmid#187830PurposePlasmid for the expression and purification of GST-TEV-EGFP-TBK1 in Spodoptera frugiperda cells (Sf9). Internal reference: SMC1631DepositorAvailable SinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV-CamKII-TRPV1-P2A-mCherry
Plasmid#200829PurposeTransduce excitatory neurons with AAV to express TRPV1 along with an mCherry fluorescence reporterDepositorAvailable SinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLIX403_UBAP2L_mCherry
Plasmid#105286Purposepuro-selectable lentivirus vector for inducible expression of a UBAP2L-mCherry fusion proteinDepositorInsertUBAP2L (UBAP2L Human)
UseLentiviralTagsmCherryExpressionMammalianPromoterTRE promoter, Tet ONAvailable SinceSept. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
GfaABC1D BARK D110A p2A mCherry
Plasmid#117692PurposeGfaABC1D-iBARK(D110A)-p2A-mCherry: Negative control viral expression vector for astrocyte Gaq silencingDepositorInsertBARKrgs D110A peptide
UseAAVTagsHA / FLAGMutationD110AAvailable SinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
sgRNA BRCA2 R2842C
Plasmid#139324PurposePlasmid expressing a sgRNA to introduce BRCA2 R2842C using base editingDepositorInsertsgRNA to insert BRCA2 R2842C using base editing
ExpressionMammalianPromoterU6Available SinceMay 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
TLCV2-Scal-T2A-EGFP-Puro
Plasmid#234949PurposeDoxycycline-inducible mitochondria-localized ScaI expressing plasmidDepositorInsertScaI restriction enzyme with N-terminal COX8 mitochondrial localization signal
UseLentiviralTagsFLAGExpressionMammalianPromotertight TRE promoterAvailable SinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
TLCV2-ApaLI-T2A-EGFP-Puro
Plasmid#234948PurposeDoxycycline-inducible mitochondria-localized ApaLI expressing plasmidDepositorInsertApaLI restriction enzyme with N-terminal COX8 mitochondrial localization signal
UseLentiviralTagsFLAGExpressionMammalianPromotertight TRE promoterAvailable SinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CBh-IgKL-mNG
Plasmid#183467PurposeTo produce AAV with the CBh promoter to express IgkL-mNeonGreen (IgKL is a secretory signal peptide)DepositorInsertIgKL-mNeonGreen
UseAAVTagsIgKL secretory signalAvailable SinceMay 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
UBE2LB_pLX307
Plasmid#98380PurposeLentiviral expression of UBE2L6DepositorAvailable SinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-ChroME2s-ST-P2A-NLS-mRuby3
Plasmid#170177PurposeCation channelrhodopsin ChroME2s targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vectorDepositorInsertChroME2s-ST-P2A-NLS-mRuby3
UseAAVExpressionMammalianAvailable SinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-MCHpr-GCaMP6f
Plasmid#221039PurposeExpresses GCaMP6f under the rat pro-melanin-concentrating hormone promoterDepositorInsertGCaMP6f
UseAAV; Rat targetingExpressionMammalianMutationA317EPromoterRat MCHAvailable SinceAug. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
FUW-tetO-EGFP
Plasmid#84041Purpose3rd generation lentiviral transfer plasmid. Expresses EGFP and puromycin resistance in mammalian cells under the control of a doxycycline-inducible promoterDepositorInsertEnhanced Green Fluorescence Protein, Puromycin resistance gene
UseLentiviralExpressionMammalianPromoterTRE-CMVAvailable SinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLV-tetO-Tfap2a
Plasmid#70274PurposeLentiviral plasmid for tetracycline/doxycycline dependent expression of murine Tfap2aDepositorAvailable SinceNov. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGAPDH::H2B-mScarlet
Plasmid#186764PurposeA plasmid containing planarian H2B fused to a planarian codon-optimized mScarlet, driven by the planarian GAPDH promoter.DepositorInsertH2B-mScarlet
UseOverexpressionTagsH2BPromoterGAPDHAvailable SinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDS170-enNTS1
Plasmid#199152PurposeJZ73, bacterial expression construct expressing thermostabilised rat neurotensin receptor 1 (enNTS1)DepositorInsertenNTS1 (Ntsr1 Rat)
Tags10xHis tag, Avi tag, MBP, and muGFPExpressionBacterialMutationV208M, C273S, C391S and C393S (over NTS1-B5)PromoterLac/T5Available SinceMay 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pmiRGLO-GLIS2-201
Plasmid#117331PurposeDual luciferase assay for miRNA-targeted UTRDepositorInsert3' UTR GLIS2-201
UseLuciferaseTagsluciferaseExpressionMammalianPromoterMurine PGK-1Available SinceDec. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGL3 Zscan4 promoter
Plasmid#69249PurposeLuciferase reporter for Dux4 activation of Zscan4 transcriptionDepositorInsertZscan4 (ZSCAN4 Human)
UseLuciferaseMutationThe two single nucleotide mismatches found betwee…PromoterZscan4Available SinceSept. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJBEI-6410
Plasmid#47049PurposeBglBrick plasmid (=pBbA5a-MTSAe-T1f-MBI(f)-T1002i-Ptrc-trGPPS(co)-LS) coding for MEV pathway enzymes to produce limonene from glucose in E. coliDepositorInsertCodon-optimized sequences for MEV pathway expression in E. coli to produce limonene
UseSynthetic Biology; BglbrickExpressionBacterialMutationR364S mutation in MKPromoterPlacUV5Available SinceSept. 12, 2013AvailabilityAcademic Institutions and Nonprofits only