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pBAV-B

Plasmid

#65928

Purpose

Theophylline Riboswitch B with lacZ gene

Depositor

Justin Gallivan

Article

Topp et al Appl Environ Microbiol. 2010 Dec;76(23):

Insert

lacZ

Expression

Bacterial

Available Since

Sept. 3, 2015

Availability

Academic Institutions and Nonprofits only
pBAV-A

Plasmid

#65927

Purpose

Theophylline Riboswitch A with lacZ gene

Depositor

Justin Gallivan

Article

Topp et al Appl Environ Microbiol. 2010 Dec;76(23):

Insert

lacZ

Expression

Bacterial

Promoter

T5

Available Since

Sept. 3, 2015

Availability

Academic Institutions and Nonprofits only
pSi

Plasmid

#67884

Purpose

Derivative of pSiM24 plant expression vector without the M24 promoter

Depositor

Indu Maiti

Article

Sahoo et al PLoS One. 2014 Jun 4;9(6):e98988. doi: 1

Type

Empty backbone

Expression

Plant

Available Since

Aug. 26, 2015

Availability

Academic Institutions and Nonprofits only

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attB-mCh-Poly(A)‐NeoR

Plasmid

#65528

Purpose

NeoR containing plasmid for the generation of gene disruptions via Bxb1-mediated recombination into MIN-tagged cell lines. This vector can also be used to express cDNAs.

Depositor

Sebastian Bultmann

Article

Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

Insert

attB-mCherry

Use

Mouse Targeting; Bxb1

Expression

Mammalian

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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EGFP-Caveolin3DGV

Plasmid

#67886

Purpose

Fluorescently tagged Caveolin-3 truncation mutant for expression in mammalian cells

Depositor

Rob Parton

Article

Luetterforst et al J Cell Biol. 1999 Jun 28;145(7):1443-59.

Insert

Cav3 (Cav3 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Deletion of the first 53 amino acids of Caveolin3

Promoter

CMV

Available Since

Aug. 19, 2015

Availability

Academic Institutions and Nonprofits only
oxBFP

Plasmid

#68063

Purpose

C-terminal fusion to oxBFP

Depositor

Erik Snapp

Article

Costantini et al Nat Commun. 2015 Jul 9;6:7670. doi: 10.1

Type

Empty backbone

Tags

oxBFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only
pBDR

Plasmid

#62663

Purpose

Donor vector for tdTomato knock-in via landing pad

Depositor

Masato Ohtsuka

Article

Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

Insert

tdTomato

Use

Cre/Lox

Available Since

Aug. 10, 2015

Availability

Academic Institutions and Nonprofits only

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130_pSKB3-LUMP-P.leioghnati

Plasmid

#65894

Purpose

Bacterial expression of P. leioghnati LUMP protein, a FRET acceptor protein emitting a cyan-colored fluorescence with an unusually long excited state lifetime

Depositor

Gerard Marriott

Article

Hoepker et al Proc Natl Acad Sci U S A. 2015 Apr 30. p

Insert

LUMP

Expression

Bacterial

Available Since

Aug. 4, 2015

Availability

Academic Institutions and Nonprofits only

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mTagRFP-T-Fibrillarin-7

Plasmid

#58016

Purpose

Localization: Nucleoli, Excitation: 555, Emission: 584

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

Fibrillarin (FBL Human)

Tags

mTagRFP

Expression

Mammalian

Mutation

R36Q, S66F

Promoter

CMV

Available Since

July 31, 2015

Availability

Industry, Academic Institutions, and Nonprofits
LOC8R-PSmOrange

Plasmid

#66897

Purpose

PSmOrange targeted to mitochondria using the synthetic LOC8R presequence

Depositor

Georg Ramm

Insert

LOC8R-PSmOrange

Tags

LOC8R

Available Since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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pQE-WT-T3-R5-RRVH

Plasmid

#63623

Purpose

T3-R5-RRVH RNAP mutant

Depositor

Andrew Ellington

Article

Meyer et al ACS Synth Biol. 2014 Oct 7.

Insert

T3 RRVH RNAP

Mutation

T745K, N748D, L749M, M750I, G753R, H772R, E775V, …

Promoter

T5/Lac

Available Since

July 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pECFP-N2-COL1A1

Plasmid

#66603

Purpose

Expresses procollagen 1 alpha 1 as a CFP fusion

Depositor

David Stephens

Article

Stephens et al J Cell Sci. 2002 Mar 15;115(Pt 6):1149-6

Insert

COL1A1 (COL1A1 Human)

Expression

Mammalian

Available Since

July 28, 2015

Availability

Academic Institutions and Nonprofits only
pFETCh_GABPA

Plasmid

#64253

Purpose

Homology Arms and 3X Flag with P2A Neo for 3' tagging of human GABPA

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

GABPA Homology Arms (GABPA Human)

Tags

3X Flag P2A Neo

Expression

Mammalian

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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pENTRY4_HuB

Plasmid

#65752

Purpose

entry vector with HuB

Depositor

Thomas Tuschl

Insert

ELAVL2 (ELAVL2 Human)

Expression

Mammalian

Available Since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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pT7-V5-SBP-C1-HsRRP4

Plasmid

#64870

Purpose

expressses V5- and SBP-tagged RRP4 in mammalian cells

Depositor

Elisa Izaurralde

Insert

RRP4 (EXOSC2 Human)

Tags

V5 and SBP

Expression

Mammalian

Promoter

CMV

Available Since

June 24, 2015

Availability

Academic Institutions and Nonprofits only

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EGFP-CERT-PH

Plasmid

#49572

Purpose

expresses the plekstrin homology domain of CERT/OSBP in mammalian cells

Depositor

Marci Scidmore

Article

Moorhead et al Infect Immun. 2010 May;78(5):1990-2007.

Insert

GPBP (CERT1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

expresses AA 1-122

Promoter

CMV

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only
Ii-Str_ManII-SBP-tagBFP

Plasmid

#65257

Purpose

synchronize trafficking of ManII from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Ii-Streptavidin and truncated Mannosidase II

Tags

Streptavidin Binding Protein (SBP) and tagBFP

Expression

Mammalian

Mutation

contains only amino acids 1 to 116 of ManII

Promoter

CMV

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR 7SK-miR-30 L1-L4

Plasmid

#62180

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a 7SK RNA polymerase III promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Type

Empty backbone

Use

Gateway Cloning and RNAi

Expression

Mammalian

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only
pMuLE ENTR H1-miR-30 L1-R5

Plasmid

#62181

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a H1 promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Type

Empty backbone

Use

Gateway Cloning and RNAi

Expression

Mammalian

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only
pMuLE ENTR U6 stuffer sgRNA scaffold L1-R5

Plasmid

#62127

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and sgRNA scaffold module for gRNA expression. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Type

Empty backbone

Use

CRISPR and Gateway Cloning

Expression

Mammalian

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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