We narrowed to 18,177 results for: multi
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Plasmid#141237PurposeAAV vector with Ef1a promoter and LoxFAS sites for Cre-Off expression of GCaMP6f (GCaMP6f will not be expressed in mammalian cells that express Cre recombinase)DepositorInsertGCaMP6f
UseAAV and Cre/LoxTags6XHIS and XpressExpressionMutationPromoterHuman elongation factor-1 alpha (EF-1 alpha)Available sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pICH47811-pAtAct2::Cas9-nls::tSlATPase
Plasmid#160221PurposeCas9-nuclear localization, pACT2 promoter, SlATPase terminator transcriptional unit, inserted at the MoClo toolkit level 1 acceptor position 2 reverseDepositorInsertpAtACT2::Cas9-nls::tSlATPase
UseSynthetic Biology; Moclo toolkit level 1 acceptor…TagsExpressionPlantMutationPromoterAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEM-T Easy-Cas9-nls-CDS1
Plasmid#160231PurposeCas9-nuclear localization, level 0 of MoClo Golden Gate position CDS1DepositorInsertCas9-nuclear localization signal gene sequence, Golden Gate MoClo position CDS1
UseLevel 0 of moclo golden gate position cds1TagsExpressionMutationPromoterAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CBA-Con/Fon-EYFP-WPRE
Plasmid#186752PurposeAAV-mediated Cre and Flp-dependent expression of EYFP under CBA promoterDepositorInsertEYFP
UseAAVTagsExpressionMutationPromoterCBAAvailable sinceJuly 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
Human_TFAM_HMGA_pET28
Plasmid#167326PurposeExpresses TFAM HMGA mutant in bacterial cellsDepositorInsertTFAM (TFAM Human)
UseTags6XHisExpressionBacterialMutationTruncation mutant containing HMGA domain (43-122)PromoterT7Available sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYZ_NAND4_L17_S24_S11_S19_S13
Plasmid#132732PurposeT7 RNAP-driven expression of a 4-input NAND gate RNA with a GFP-ASV reporter that binds to 3WJ repressor trigger indices 24, 11, 19, and 13DepositorInsert3WJ_NAND4_L17_S24_S11_S19_S13
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAG_ToeRep_N09_switch
Plasmid#132741PurposeT7 RNAP-driven expression of first-generation toehold repressor index 9 switch RNA with a GFP-ASV reporterDepositorInsertToeRep_N09_switch
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAG_Ptet*_ToeRep_N01_switch
Plasmid#132737PurposeInducible expression of first-generation toehold repressor index 1 switch RNA with a GFP-ASV reporter via the Ptet* promoterDepositorInsertPtet*_ToeRep_N01_switch
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJK_ToeRepG2_N19_switch
Plasmid#132744PurposeT7 RNAP-driven expression of second-generation toehold repressor index 19 switch RNA with a GFP-ASV reporterDepositorInsertToeRepG2_N19_switch
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJK_ToeRepG2_N64_switch
Plasmid#132743PurposeT7 RNAP-driven expression of second-generation toehold repressor index 64 switch RNA with a GFP-ASV reporterDepositorInsertToeRepG2_N64_switch
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYZ_Ptet*_3WJrep_N19_switch
Plasmid#132739PurposeInducible expression of 3WJ repressor index 19 switch RNA with a GFP-ASV reporter via the Ptet* promoterDepositorInsertPtet*_3WJrep_N19_switch
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYZ_NAND3_L11_S11_S13_S19
Plasmid#132731PurposeT7 RNAP-driven expression of a 3-input NAND gate RNA with a GFP-ASV reporter that binds to 3WJ repressor trigger indices 11, 13, and 19DepositorInsert3WJ_NAND3_L11_S11_S13_S19
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-1
Plasmid#103160PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-1 target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-1 target
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-30a(+)-N-SH2
Plasmid#111274Purposeexpress murine Syk (N)SH2 domain (Ser 8 to Gly 117), with an N-terminal (His)6 tag and TEV cleavage site, in E.coli strain Rosetta 2 (DE3)DepositorInsertmurine Syk (N)SH2 domain (Ser 8 to Gly 117), with an N-terminal (His)6 tag and TEV cleavage site (Syk Mouse)
UseTagsHHHHHHENLYFQGExpressionBacterialMutationPromoterT7 promoterAvailable sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMOD_B3200
Plasmid#91079PurposeModule B, Promoter: none (begins with P2A to be fused to TALE-VPR_1), Gene: TALE-VPR_2 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: HSPDepositorInsertTALE-VPR_2 backbone with Esp3I ccdb cassette for repeat cloning
UseTALENTagsExpressionMutationPromoternone (begins with P2A to be fused to TALE-VPR_1)Available sinceJuly 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD_B3400
Plasmid#91080PurposeModule B, Promoter: none (begins with P2A to be fused to TALE-VP64_1), Gene: TALE-VP64_2 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: HSPDepositorInsertTALE-VP64_2 backbone with Esp3I ccdb cassette for repeat cloning
UseTALENTagsExpressionMutationPromoternone (begins with P2A to be fused to TALE-VP64_1)Available sinceJuly 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A3101
Plasmid#91048PurposeModule A, Promoter: 35S, Gene: TALE-VPR_1 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: none (ends with P2A to be fused to TALE-VPR_ 2)DepositorInsertTALE-VPR_1 backbone with Esp3I ccdb cassette for repeat cloning
UseTALENTagsExpressionMutationPromoter35SAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A3301
Plasmid#91049PurposeModule A, Promoter: 35S, Gene: TALE-VP64_1 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: none (ends with P2A to be fused to TALE-VP64_ 2)DepositorInsertTALE-VP64_1 backbone with Esp3I ccdb cassette for repeat cloning
UseTALENTagsExpressionMutationPromoter35SAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTaCas9-dead_6
Plasmid#91175PurposeCas9 only expression, Cas9 Type: TaCas9_D10A+H840A (dead), Plant Selection: PvUbi2:barDepositorInsertTaCas9_D10A+H840A (dead)
UseCRISPRTagsExpressionPlantMutationD10A, H840APromoterAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTaCas9-dead_5
Plasmid#91174PurposeCas9 only expression, Cas9 Type: TaCas9_D10A+H840A (dead), Plant Selection: PvUbi2:hpt IIDepositorInsertTaCas9_D10A+H840A (dead)
UseCRISPRTagsExpressionPlantMutationD10A, H840APromoterAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only