We narrowed to 37,610 results for: ELL
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Plasmid#171814PurposeUniversal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein and blasticidin resistance cassette [p.M1G]DepositorInsertmScarlet-T2A-BlastR
UseGene taggingMutationBlastR: Changed Methionin 1 to Glycine.Available sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAS_4xU6-PylT M15 (UUA) sfGFP 150TAA
Plasmid#154775Purposeplasmid with 4xMma PylT (M15 mutant, UUA anticodon) cassette and ochre suppression reporter sfGFP 150 TAA stop, for transient transfection or stable, piggybac-mediated, integrationDepositorInsertsfGFP
ExpressionMammalianMutation150TAA in GFP reporter, UUA anticodon and G10A, …PromoterEF1Available sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
C1-MPAct(delta1-6)-mRuby3
Plasmid#155223PurposeF-actin bidning mutant of MPActDepositorInsertMutated version of F-tractin (aa 15-52 of rat ITPKA) fused to mRuby3 C-term tagged with the CaaX sequence derived from Kras4b
TagsmRuby3ExpressionMammalianMutationDeletion of first 6 AA from F-tractin (9-14 from …Available sinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJ204-loxP-EF1a-mCherry-NeoR-lox2272-BGHpA-siteA-HDR
Plasmid#154826PurposeLanding pad for Cre/lox-based recombinase-mediated cassette exchange (RMCE) in CHO cells, donor plasmid for CRISPR/Cas9-mediated targeted integration in CHO cells (site A)DepositorInsertsmCherry
NeoR
ZsGreen1-DR
UseCRISPR and Cre/LoxTagsPESTExpressionMammalianPromoterCMV, EF1a, and SV40 early promoterAvailable sinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-Halotag-loxP-P2A-dTKneo-pA-loxP
Plasmid#139485PurposePCR template for Halo-Tag gene knock-in donor vectorDepositorInsertHaloTag-loxP-P2A-dTKneo-pA-loxP
TagsHaloTagExpressionMammalianPromoterCAG promoterAvailable sinceApril 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-mEGFP-loxP-P2A-dTKneo-pA-loxP
Plasmid#139484PurposePCR template for mEGFP gene knock-in donor vectorDepositorInsertmEGFP-loxP-P2A-dTKneo-pA-loxP
TagsmEGFPExpressionMammalianPromoterCAG promoterAvailable sinceApril 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-Bi-gePSI-pCamk2a-AcGFP
Plasmid#133732PurposeExpresses both the gePSI-α-chain and the gePSI-β-chain under control of the inducible bi-directional TRE3G promoter. Expresses constitutively AcGFP under control of a Camk2a promoter.DepositorInsertsgePSI-β-chain
gePSI-α-chain
AcGFP1
UseAAVTagsmurine ornithine decarboxylase - degron (ODC36)ExpressionMammalianPromoterpCamk2a and pTRE3G-BiAvailable sinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
GFP-SBP-VAP-A*
Plasmid#120175PurposeExpresses a chimera of GFP (fluorescent tag), SBP tag and the C-terminal of ER-resident protein VAP-ADepositorInsertVAMP associated protein A (VAPA Human)
TagsEGFP and SBPExpressionMammalianMutationTruncated VAP-A: 118-249PromoterCMVAvailable sinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACT1:Cas9-GFP, U6:sgUPRT
Plasmid#122853PurposeExpresses Cas9 fused with GFP and a sgRNA targeting the Cryptosporidium parvum UPRT geneDepositorInsertsCas9-GFP
U6-sgUPRT
UseCRISPR; Cas9-gfp plasmid for genome editing of cr…TagseGFPPromoterCryptosporidium parvum U6 and Cryptosporidium par…Available sinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pmCherry-T2A-moxMaple3
Plasmid#120875PurposeMammalian expression of mCherry and moxMaple3DepositorInsertsmCherry
moxMaple3
ExpressionMammalianMutationfrom mMaple3, moxMaple3 was engineered by replaci…Available sinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLenti CMV-MRLC1-mRuby2-IRES-PuroR
Plasmid#103031PurposeLentiviral expression of mRuby2-tagged MRLC with puromycin selectionDepositorAvailable sinceMay 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pssGFP-hCRT
Plasmid#80022PurposeMammalian expression of signal peptide-EGFP-humanCRTDepositorInsertCRT (CALR Human)
TagsEGFP and signal peptideExpressionMammalianMutationsignal peptide 17aa added before GFP-CRT, will ta…PromoterCMVAvailable sinceJuly 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
FUW TetO HA-AMPKa1 AS
Plasmid#69825PurposeExpresses AMPK alpha 1 in mammalian cells in a doxycycline inducible mannerDepositorInsert5'-AMP-activated protein kinase catalytic subunit alpha-1 (PRKAA1 Human)
UseLentiviralTagsHAExpressionMammalianPromoterminimal CMV promoter with tetracycline operatorAvailable sinceMay 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTT3 Flrt1-myc
Plasmid#72191PurposeExpress full-length Flrt1 with a C-terminal myc tagDepositorAvailable sinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pscAluYNF1
Plasmid#50934PurposeExpresses scAluY RNA in mammalian cells.DepositorInsertscAluYNF1
ExpressionMammalianMutationthe poly(A) linker and the right arm of the dimer…Promoter7SL RNA promoter (pol III)Available sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
RIPR-bio-His
Plasmid#50824PurposeExpresses enzymatically monobiotinylated full length RIPRDepositorInsertRIPR
TagsratCD4d3+4,biotinylation sequence, 6xHisExpressionMammalianMutationall potential N-linked glycosylation sites (N-X-S…PromoterCMVAvailable sinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
HMGA2(3'UTR DEL)+GFP
Plasmid#25406DepositorPublicationInserthigh mobility group AT-Hook 2 (Hmga2 Mouse)
UseLentiviralExpressionMammalianMutation3" UTR is absentAvailable sinceJuly 14, 2010AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRQ-hRasV12
Plasmid#18113DepositorInserth-RasV12G (HRAS Human)
UseRetroviralExpressionMammalianMutationG12V, constitutively activeAvailable sinceFeb. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
pePEmax-pPuro
Plasmid#215688PurposeCloning vector for Uni-Vector prime editing systemDepositorInsertPEmax
UseUnspecifiedPromoterEFS promoterAvailable sinceDec. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
delCMV-mCherry-3x-p67phox-GBD
Plasmid#214141PurposeRac activity sensorDepositorInsert3xp67phox-GBD (NCF2 Human)
TagsmCherryExpressionMammalianMutationOnly the GTPase binding domain (GBD) is includedPromoterdelCMVAvailable sinceApril 12, 2024AvailabilityAcademic Institutions and Nonprofits only