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pQC MCS IRES Puro

Plasmid

#110343

Purpose

γ-Retroviral transfer vector for cloning and expressing your gene of interest. IRES-driven Puromycin selection.

Depositor

Sandra Martha Gomes Dias

Article

Adamoski et al Nat Struct Mol Biol. 2023 Dec;30(12):190

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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Tubb3gRNA-mCherry

Plasmid

#87111

Purpose

mouse Tubb3 target gRNA expression plasmid with CAGmCherry

Depositor

Juan Belmonte

Article

Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

Insert

Mouse Tubb3 gRNA

Use

CRISPR

Promoter

CAG

Available Since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

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AOI-WT-Cas9-sg-mouse Ezh2-E18-GFP

Plasmid

#91880

Purpose

Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Ezh2

Depositor

Martine Roussel

Article

Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

Insert

gRNA targeting Ezh2 (Ezh2 Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

June 26, 2017

Availability

Academic Institutions and Nonprofits only
mHDAC7-Spliced (1-938) pEF6-Flag (MJS_015)

Plasmid

#73268

Purpose

[Edit] Expression of mouse HDAC7-S (1-938) in mammalian cells (Flag tag)

Depositor

Matthew Sweet

Article

Shakespear et al J Biol Chem. 2013 Aug 30;288(35):25362-7

Insert

HDAC7 (Hdac7 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pBFC0694

Plasmid

#186693

Purpose

ShDART under control of Lac promoter, original configuration (pre-gRNA terminator and J23119 promoter), Sh_lacZ_α_1 gRNA, GmR+sfGFP cargo

Depositor

Jennifer Doudna

Article

Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:

Inserts

tnsB
tnsC
tniQ
Cas12k
Sh_lacZ_α_1 gRNA
Tn7 transposon
GmR+sfGFP ShDART Cargo

Expression

Bacterial

Promoter

PLac

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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mHDAC7-Spliced (1-938) pEF6-V5 (MJS_014) [Edit]

Plasmid

#73267

Purpose

Expression of mouse HDAC7-S (1-938) in mammalian cells (V5 tag)

Depositor

Matthew Sweet

Article

Shakespear et al J Biol Chem. 2013 Aug 30;288(35):25362-7

Insert

HDAC7 (Hdac7 Mouse)

Tags

His/V5

Expression

Mammalian

Available Since

Feb. 10, 2016

Availability

Academic Institutions and Nonprofits only

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RAT

Plasmid

#187416

Purpose

Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

Depositor

Susanne Dobler

Article

Mohammadi et al PLoS Genet. 2022 Aug 16;18(8):e1010323.

Inserts

ATP1A1 (Atp1a1 Rat)
ATP1B1 (Atp1b1 Rat)

Expression

Insect

Promoter

PH and p10

Available Since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA4-His-Max-A-PQBP1-Y65C

Plasmid

#25803

Depositor

Marius Sudol

Article

Tapia et al J Biol Chem. 2010 Jun 18;285(25):19391-4

Insert

PQBP1 with Y65C mutation (PQBP1 Human)

Tags

His and Xpress

Expression

Mammalian

Mutation

Tyrosine 65 within the WW domain of PQBP1 is chan…

Available Since

July 21, 2010

Availability

Academic Institutions and Nonprofits only
pLEX307_PDHA2S293A

Plasmid

#115197

Purpose

Lentiviral transduction and expression of PDHA2S293A into any mammalian cell

Depositor

Mohan Babu

Article

Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

Insert

PDHA2S293A (PDHA2 Human)

Use

Lentiviral

Mutation

p.S293A

Promoter

EF1alpha

Available Since

March 26, 2021

Availability

Academic Institutions and Nonprofits only

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PTRF/Cavin1_L (OZ513)

Plasmid

#27188

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting PTRF/Cavin1 (cavin1b Zebrafish)

Use

Zebrafish targeting

Available Since

Feb. 8, 2011

Availability

Academic Institutions and Nonprofits only
pPHAGE-EF1α-Luc-C20orf24-3’UTR-WT

Plasmid

#128551

Purpose

Lentiviral constitutive expression of Firefly luciferase under control of WT 3'UTR of human C20orf24.

Depositor

Mohan Babu

Article

Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

Insert

Firefly luciferase

Use

Lentiviral and Luciferase

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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mHDAC7-Unspliced (23-938) pEF6-V5 (MJS_017)

Plasmid

#73266

Purpose

Expression of mouse HDAC7-U (23-938) in mammalian cells (V5 tag)

Depositor

Matthew Sweet

Article

Shakespear et al J Biol Chem. 2013 Aug 30;288(35):25362-7

Insert

HDAC7 (Hdac7 Mouse)

Tags

His/V5

Expression

Mammalian

Available Since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pChuk

Plasmid

#87033

Purpose

express IKKalpha in mammalian cells

Depositor

Georgios Stathopoulos

Article

Marazioti et al Nat Commun. 2018 Feb 14;9(1):672. doi: 1

Insert

IKKalpha (Chuk Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

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pMT-HGP-v3-NbVHH05-hIgG

Plasmid

#171565

Purpose

Expresses hIgG-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-human IgG-TEV-Avi-His) in fly cells

Depositor

Norbert Perrimon, Hidde Ploegh

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

NbVHH05-human IgG-TEV-Avi-His (UBE2J1 Human)

Tags

BiP signal peptide and hIgG1-hinge-N297A-TEV site…

Expression

Insect

Available Since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

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pQC MCS IRES G418

Plasmid

#110344

Purpose

γ-Retroviral transfer vector for cloning and expressing your gene of interest. IRES-driven Geneticin selection.

Depositor

Sandra Martha Gomes Dias

Article

Adamoski et al Nat Struct Mol Biol. 2023 Dec;30(12):190

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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MmCavin4-APEX2-P2A-mKate2

Plasmid

#170524

Purpose

Vector for expression of MmCavin4-APEX2-P2A-mKate2 in mammalian cells. Parton lab clone EJF

Depositor

Rob Parton

Article

Rae et al Elife. 2021 Apr 27;10. pii: 64630. doi:

Insert

Cavin4 (Cavin4 Mouse)

Tags

APEX2-P2A-mKate2

Expression

Mammalian

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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mHDAC7-Nterm (23-504) pEF6-V5

Plasmid

#73270

Purpose

Expression of mouse HDAC7-Nterm in mammalian cells (V5 tag)

Depositor

Matthew Sweet

Article

Shakespear et al J Biol Chem. 2013 Aug 30;288(35):25362-7

Insert

HDAC7 (Hdac7 Mouse)

Tags

His/V5

Expression

Mammalian

Mutation

deleted amino acids 505-938

Available Since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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mHDAC7-Unspliced (23-938) pEF6-Flag (MJS_018)

Plasmid

#73269

Purpose

mHDAC7-U (23-938) pEF6-V5 (MJS_017) (Flag tag)

Depositor

Matthew Sweet

Article

Shakespear et al J Biol Chem. 2013 Aug 30;288(35):25362-7

Insert

HDAC7 (Hdac7 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pitx2_R (OZ534)

Plasmid

#27211

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting pitx2 (pitx2 Zebrafish)

Use

Zebrafish targeting

Available Since

Feb. 25, 2011

Availability

Academic Institutions and Nonprofits only
pitx2_L (OZ533)

Plasmid

#27210

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting pitx2 (pitx2 Zebrafish)

Use

Zebrafish targeting

Available Since

Jan. 25, 2011

Availability

Academic Institutions and Nonprofits only