We narrowed to 1,263 results for: nms

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pUC57-sgRNA expression vector

Plasmid

#51132

Purpose

For in vitro transcription of sgRNA from the T7 promoter.

Depositor

Xingxu Huang

Article

Shen et al Nat Methods. 2014 Mar 2. doi: 10.1038/nm

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

T7

Available Since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE

Plasmid

#32702

Purpose

Conditional overexpression vector. Deletion of dsRed by Cre recombinase results in the rapid loss of dsRed and the activation of your gene fused to eGFP expression.

Depositor

Hans Clevers

Article

Koo et al Nat Methods. 2011 Dec 4. doi: 10.1038/nm

Insert

eGFP

Use

Cre/Lox and Retroviral

Expression

Mammalian

Available Since

Dec. 13, 2011

Availability

Academic Institutions and Nonprofits only

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pDD122 (Peft-3::Cas9 + ttTi5605 sgRNA)

Plasmid

#47550

Purpose

Cas9 + sgRNA plasmid that is targeted to a genomic site near the ttTi5605 Mos1 insertion allele

Depositor

Bob Goldstein

Article

Dickinson et al Nat Methods. 2013 Sep 1. doi: 10.1038/nm

Inserts

Cas9
ttTi5605 sgRNA

Use

CRISPR

Tags

HA and NLS

Expression

Worm

Mutation

Codon optimized and with synthetic introns for C.…

Promoter

U6 and eef-1A.1 (eft-3)

Available Since

Sept. 9, 2013

Availability

Academic Institutions and Nonprofits only

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mRuby2-Lysosomes-20

Plasmid

#55902

Purpose

Localization: Lysosome Membrane, Excitation: 559, Emission: 600

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

LAMP1

Tags

mRuby2

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pET21a - NDH

Plasmid

#89626

Purpose

Expresses recombinant 6HIS tagged T. cruzi NADP-dependent oxidoreductase in E. coli upon IPTG induction

Depositor

Judy Lieberman

Article

Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

Insert

NAD(P)-dependent oxidoreductase

Tags

6HIS and T7 tag (gene 10 leader)

Expression

Bacterial

Available Since

April 20, 2017

Availability

Academic Institutions and Nonprofits only

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pET21a - EtfA

Plasmid

#89616

Purpose

Expresses recombinant 6HIS tagged T. cruzi electron transfer flavoprotein A in E. coli upon IPTG induction

Depositor

Judy Lieberman

Article

Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

Insert

Electron transport flavoprotein alpha subunit

Tags

6HIS and T7 tag (gene 10 leader)

Expression

Bacterial

Available Since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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pET21a - EtfB

Plasmid

#89617

Purpose

Expresses recombinant 6HIS tagged T. cruzi electron transfer flavoprotein B in E. coli upon IPTG induction

Depositor

Judy Lieberman

Article

Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

Insert

electron-transfer-flavoprotein, alpha polypeptide

Tags

6HIS and T7 tag (gene 10 leader)

Expression

Bacterial

Available Since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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pET21a - NFDD

Plasmid

#89624

Purpose

Expresses recombinant 6HIS tagged T. cruzi NAD-FAD–dependent dehydrogenase in E. coli upon IPTG induction

Depositor

Judy Lieberman

Article

Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

Insert

NAD/FAD dependent dehydrogenase

Tags

6HIS and T7 tag (gene 10 leader)

Expression

Bacterial

Available Since

April 20, 2017

Availability

Academic Institutions and Nonprofits only

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pET21a - TR

Plasmid

#89621

Purpose

[Edit] Expresses recombinant 6HIS tagged T. cruzi trypanothione reductase protein in E. coli upon IPTG induction

Depositor

Judy Lieberman

Article

Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

Insert

Thioredoxin

Tags

6HIS and T7 tag (gene 10 leader)

Expression

Bacterial

Available Since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Camui-CR

Plasmid

#40256

Depositor

Michael Lin

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

Camuiα-CR

Expression

Mammalian

Promoter

CMV IE

Available Since

Oct. 4, 2012

Availability

Academic Institutions and Nonprofits only

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pET21a - SodA

Plasmid

#89612

Purpose

Expresses recombinant 6HIS tagged T. cruzi superoxide dismutase A protein in E. coli upon IPTG induction

Depositor

Judy Lieberman

Article

Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

Insert

Superoxide dismutase A

Tags

6HIS and T7 tag (gene 10 leader)

Expression

Bacterial

Available Since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-TDsmURFP-IRES-HO-1

Plasmid

#80346

Purpose

Expresses Tandem Dimer smURFP and Heme Oxygenase-1 in mammalian cells.

Depositor

Erik Rodriguez, Roger Tsien

Article

Rodriguez et al Nat Methods. 2016 Aug 1. doi: 10.1038/nm

Inserts

Tandem Dimer smURFP
HO-1

Expression

Mammalian

Promoter

CMV and IRES

Available Since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-CASI-Cas9C-P2A-turboGFP

Plasmid

#80942

Purpose

Expresses Cas9C in mammalian cells; co-expresses turboGFP.

Depositor

George Church

Article

Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

Insert

Cas9C

Use

AAV, CRISPR, and Synthetic Biology

Expression

Mammalian

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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mRuby2-Rab5a-7

Plasmid

#55911

Purpose

Localization: Ras GTPase, Excitation: 559, Emission: 600

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

Rab5a

Tags

mRuby2

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pCry2PHR-mCh-RhoA

Plasmid

#42958

Purpose

Expresses optogenetically controlled RhoA

Depositor

David Schaffer

Article

Bugaj et al Nat Methods. 2013 Feb 3. doi: 10.1038/nm

Insert

Cry2PHR-mCherry-RhoA (RHOA Human, Mustard Weed)

Expression

Mammalian

Promoter

CMV

Available Since

April 9, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3-AKAR2-CR

Plasmid

#40255

Depositor

Michael Lin

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

AKAR2

Tags

His6 and Xpress

Expression

Mammalian

Promoter

CMV IE

Available Since

Oct. 4, 2012

Availability

Academic Institutions and Nonprofits only

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pDS275

Plasmid

#34972

Purpose

Expresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep(T406A, T407A, I532A mutations).

Depositor

Michael Glotzer

Article

Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

Insert

Mid2(SS/TM)-GFP-LOVpep

Expression

Yeast

Mutation

T406A, T407A, I532A

Available Since

March 23, 2012

Availability

Academic Institutions and Nonprofits only

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pBAD-TDsmURFP-RBS-HO-1

Plasmid

#80342

Purpose

Expresses Tandem Dimer smURFP and Heme Oxygenase-1 in bacterial cells.

Depositor

Erik Rodriguez, Roger Tsien

Article

Rodriguez et al Nat Methods. 2016 Aug 1. doi: 10.1038/nm

Inserts

Tandem Dimer smURFP
HO-1

Tags

6 Histidine Tag

Expression

Bacterial

Promoter

pBAD

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCry2PHR-mCh-Rac1

Plasmid

#42954

Purpose

Expresses optogenetically controlled Rac1

Depositor

David Schaffer

Article

Bugaj et al Nat Methods. 2013 Feb 3. doi: 10.1038/nm

Insert

Cry2PHR-mCherry-Rac1 (RAC1 Human, Mustard Weed)

Expression

Mammalian

Promoter

CMV

Available Since

April 9, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-SMVP-Cas9N-U6-gRNA M3

Plasmid

#80936

Purpose

Expresses Cas9N in mammalian cells; expresses gRNA M3 for Mstn cleavage.

Depositor

George Church

Article

Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

Insert

Cas9N

Use

AAV, CRISPR, and Synthetic Biology

Expression

Mammalian

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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