We narrowed to 6,025 results for: plasmid dna

1. pcDNA5/FRT-HA-rM3D(Gs)

   Plasmid

   #45549

   Purpose

   Expresses rM3D (Gs) neuronal activator

   Depositor

   Bryan Roth

   Article

   Armbruster et al Proc Natl Acad Sci U S A. 2007 Mar 20;10

   Insert

   rM3D (Gs)
   

   Use

   Tags

   HA

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-ACE2-GFP

   Plasmid

   #154962

   Purpose

   pcDNA3.1-ACE2-GFP

   Depositor

   Utpal Pajvani

   Article

   Bartolome et al Sci Rep. 2021 May 7;11(1):9803. doi: 10.

   Insert

   ACE2-GFP (ACE2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-SARS-Spike

   Plasmid

   #145031

   Purpose

   Express SARS-CoV spike protein with C9 tag at C-terminal in mammalian cells

   Depositor

   Fang Li

   Article

   Shang et al Nature. 2020 Mar 30. pii: 10.1038/s41586

   Insert

   SARS-CoV Spike
   

   Use

   Tags

   C9 and CD5 signal peptide

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 16, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pcDNA3-HCMVgH (TB40/E)

   Plasmid

   #136446

   Purpose

   Mammalian expression plasmid for HCMV glycoprotein H (TB40/E strain), full-length

   Depositor

   Erik Procko

   Article

   Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

   Insert

   glycoprotein H (UL75 Human betaherpesvirus 5)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Codon-optimized for human cell expression.

   Promoter

   CMV

   Available since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-FLAG-Lin28A

   Plasmid

   #51371

   Depositor

   Narry Kim

   Article

   Heo et al Mol Cell. 2008 Oct 24;32(2):276-84. doi:

   Insert

   Lin28A (LIN28A Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-endo-Nb80

   Plasmid

   #205518

   Purpose

   Endosome-targeted Nb80 nanobody that binds and stabilizes β2AR active conformation, blocking Gαs coupling.

   Depositor

   Jin Zhang

   Article

   Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

   Insert

   endo-Nb80
   

   Use

   Tags

   2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and mChe…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.4_1G11F2-3 light chain

   Plasmid

   #198254

   Purpose

   recombinant expression of light chain of monoclonal anti-AMP antibody 1G11F2-3

   Depositor

   Aymelt Itzen

   Article

   Hopfner et al iScience. 2020 Nov 13;23(12):101800. doi

   Insert

   light chain of monoclonal anti-AMP antibody 1G11F2-3 (mouse kappa)
   

   Use

   Tags

   IL10 signal peptide

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3-endo-Nb37

   Plasmid

   #205514

   Purpose

   Endosome-targeted Nb37 nanobody that binds and stabilizes the active form of Gαs G-protein subunit.

   Depositor

   Jin Zhang

   Article

   Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

   Insert

   endo-Nb37
   

   Use

   Tags

   2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and mChe…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3-nuc-Nb80

   Plasmid

   #205519

   Purpose

   Nuclear-targeted Nb80 nanobody that binds and stabilizes β2AR active conformation, blocking Gαs coupling.

   Depositor

   Jin Zhang

   Article

   Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

   Insert

   nuc-Nb80
   

   Use

   Tags

   Nuclear localization signal (NLS) and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3-nuc-Nb37

    Plasmid

    #205515

    Purpose

    Nuclear-targeted Nb37 nanobody that binds and stabilizes the active form of Gαs G-protein subunit.

    Depositor

    Jin Zhang

    Article

    Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

    Insert

    nuc-Nb37
    

    Use

    Tags

    Nuclear localization signal (NLS) and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-GoldenGate-VP64

    Plasmid

    #47389

    Purpose

    Expression plasmid for TALE proteins fused to VP64 activation domain compatible with GoldenGate cloning system

    Depositor

    Charles Gersbach

    Article

    Perez-Pinera et al Nat Methods. 2013 Mar;10(3):239-42. doi:

    Type

    Empty backbone

    Use

    TALEN

    Tags

    FLAG, HA, and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. DNA-CARzeta-IRESpuro

    Plasmid

    #89345

    Purpose

    2nd generation lentiviral vector which expresses DNA-CAR receptor upstream of a IRES-puro cassette

    Depositor

    Ron Vale

    Article

    Taylor et al Cell. 2017 Mar 23;169(1):108-119.e20. do

    Insert

    DNA-CARzeta
    

    Use

    Lentiviral

    Tags

    SNAPf tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.4_7C11-1 light chain

    Plasmid

    #198252

    Purpose

    recombinant expression of light chain of monoclonal anti-AMP antibody 7C11-1

    Depositor

    Aymelt Itzen

    Article

    Hopfner et al iScience. 2020 Nov 13;23(12):101800. doi

    Insert

    light chain of monoclonal anti-AMP antibody 7C11-1 (mouse kappa)
    

    Use

    Tags

    IL10 signal peptide

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.4_7C11-1 heavy chain

    Plasmid

    #198251

    Purpose

    recombinant expression of heavy chain of monoclonal anti-AMP antibody 7C11-1

    Depositor

    Aymelt Itzen

    Article

    Hopfner et al iScience. 2020 Nov 13;23(12):101800. doi

    Insert

    heavy chain of monoclonal anti-AMP antibody 7C11-1 (mouse IgG2a)
    

    Use

    Tags

    IL10 signal peptide

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1-flag-IARS2-Δ1-48

    Plasmid

    #232341

    Purpose

    Expresses human IARS2 with mito-location signal replaced by flag tag in mammalian cells, also carrying synonymous mutations described in Addgene #232340

    Depositor

    Quanfu Ma

    Insert

    IARS2 (IARS2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Residues 1-48 (indicated as Mito-signal in UniPro…

    Promoter

    CMV

    Available since

    June 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.4_1G11F2-3 heavy chain

    Plasmid

    #198253

    Purpose

    recombinant expression of heavy chain of monoclonal anti-AMP antibody 1G11F2-3

    Depositor

    Aymelt Itzen

    Article

    Hopfner et al iScience. 2020 Nov 13;23(12):101800. doi

    Insert

    heavy chain of monoclonal anti-AMP antibody 1G11F2-3 (mouse IgG2b)
    

    Use

    Tags

    IL10 signal peptide

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. miR-722 pCDNA

    Plasmid

    #97162

    Purpose

    luciferase assay

    Depositor

    Qing Deng

    Article

    Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

    Insert

    miR-722 (mir722 Zebrafish)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA5 FRT/TO Plk4-mCherry

    Plasmid

    #80269

    Purpose

    mammalian expression plasmid for Plk4-mCherry

    Depositor

    Andrew Holland

    Article

    Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

    Insert

    Plk4 (PLK4 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3 RLUC POLIRES FLUC

    Plasmid

    #45642

    Purpose

    Bicistronic reporter plasmid expressing Renilla luciferase and firefly luciferase in mammalian cells.

    Depositor

    Nahum Sonenberg

    Article

    Poulin et al J Biol Chem. 1998 May 29;273(22):14002-7

    Insert

    RLUC - IRES - FLUC
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1_3xFlagEzh2 RNA binding mutant

    Plasmid

    #173718

    Purpose

    RNA-binding mutant EZH2 expression plasmid with 3XFLAG tag

    Depositor

    Thomas Cech

    Article

    Long et al Nat Genet. 2020 Sep;52(9):931-938. doi:

    Insert

    EZH2 RNA-binding mutant
    

    Use

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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