167,948 results

1. pRK5-myc-Cdc42-wt

   Plasmid

   #12972

   Depositor

   Gary Bokoch

   Insert

   Cdc42 (CDC42 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Available Since

   Oct. 27, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLL2787

   Plasmid

   #15460

   Depositor

   Chia Lee

   Article

   Luong et al J Microbiol Methods. 2007 Jul . 70(1):18

   Insert

   phage phi11 int gene
   

   Expression

   Bacterial

   Available Since

   Oct. 9, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. TARDBP_Halo_N_allele

   Plasmid

   #178131

   Purpose

   Donor vector for endogenous tagging of human TARDBP at the N-terminus with halotag

   Depositor

   Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

   Insert

   Halotag (TARDBP Human)
   

   Use

   Donor vector

   Available Since

   Dec. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pOpen-DpnIR

   Plasmid

   #165529

   Purpose

   Used to digest methylated DNA; to be expressed in dam- E. coli strains (strains which do not methylate their genomic DNA)

   Depositor

   Jennifer Molloy, FreeGenes Project, Drew Endy

   Insert

   DpnI
   

   Use

   Synthetic Biology

   Available Since

   Aug. 5, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pET15bpATnp

   Plasmid

   #121137

   Purpose

   This vector encode a His6-tagged proteinA-Tnp(hyperactive form) fusion protein.

   Depositor

   Keji Zhao

   Article

   Carter et al Nat Commun. 2019 Aug 20;10(1):3747. doi:

   Insert

   Protein A-Tnp
   

   Tags

   his

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. LentiCRISPR v2-sgLKB1 clone2

   Plasmid

   #162126

   Purpose

   Lentiviral sgRNA plasmid targeting human LKB1

   Depositor

   Boyi Gan

   Article

   Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

   Insert

   sgLKB1 (STK11 Human)
   

   Use

   Lentiviral

   Available Since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFP-SF2

   Plasmid

   #17990

   Depositor

   Tom Misteli

   Article

   Phair et al Nature. 2000 Apr 6. 404(6778):604-9.

   Insert

   SF2 (SRSF1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   contains SF2 (aa1-210)

   Available Since

   May 13, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKLV2-U6gRNA5(Empty)-PGKBFP2AGFP-W

   Plasmid

   #67979

   Purpose

   Cas9 activity reporter (control) with BFP and GFP.

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Insert

   U6gRNA cassette, PGKBFP2AGFP cassette, WPRE
   

   Use

   Lentiviral

   Mutation

   Deleted BbsI site within WPRE

   Promoter

   human U6 promoter, mouse PGK promoter

   Available Since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRel-Hyg

   Plasmid

   #227582

   Purpose

   Release plasmid with Cas9 working cassette, hygromycin resistance, and pSG5 replicon

   Depositor

   Chengzhang Fu

   Article

   Xie et al Science. 2024 Dec 13;386(6727):eabq7333.

   Insert

   Cas9
   

   Use

   CRISPR

   Available Since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDONR-A-HYG

    Plasmid

    #29635

    Depositor

    Scott Gold, David_L Andrews

    Article

    Garcia-Pedrajas et al Fungal Genet Biol. 2008 Apr . 45(4):379-

    Type

    Empty backbone

    Use

    Gateway donor

    Available Since

    Oct. 5, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. 2C-EGFP

    Plasmid

    #69071

    Purpose

    Expresses EGFP under MERVL_LTR

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Ishiuchi et al Nat Struct Mol Biol. 2015 Aug 3. doi: 10

    Insert

    EGFP
    

    Expression

    Mammalian

    Available Since

    Sept. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-CD63-L7Ae

    Plasmid

    #180503

    Purpose

    Human CD63-L7Ae fusion protein

    Depositor

    Masaharu Somiya

    Article

    Somiya et al bioRxiv 2022.01.25.477620

    Insert

    Human CD63-L7Ae fusion protein
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEGFP C3-YAP2

    Plasmid

    #19055

    Depositor

    Marius Sudol

    Article

    Oka et al J Biol Chem. 2008 Oct 10;283(41):27534-2

    Insert

    Yes-kinase associated protein (YAP1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Sept. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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14. V5-BS2-NES (Cytosol)

    Plasmid

    #220341

    Purpose

    Mammalian expression vector BS2 in cytoplasm

    Depositor

    Bryan Dickinson

    Article

    Pani et al Nat Chem. 2024 Apr 9. doi: 10.1038/s4155

    Insert

    BS2
    

    Tags

    NES (Nuclear exclusion sequence) and V5 tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 26, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pTRANS_253

    Plasmid

    #91125

    Purpose

    Transformation, Type: T-DNA, Plant Selection: PvUbi2:hpt II, Viral Replication: WDV

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    35S

    Available Since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. Cav-RA

    Plasmid

    #209881

    Purpose

    Dimerization dependent fluorescent protein RA anchored to cytosolic face of the caveolae with mouse CAVIN1

    Depositor

    Sarah Cohen

    Article

    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

    Insert

    ddRFP A
    

    Tags

    CAVIN1

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. TMED9-bio-His

    Plasmid

    #51855

    Purpose

    Expresses full-length Transmembrane emp24 domain-containing protein 9 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    TMED9 (TMED9 Human)
    

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. iCAP-ROOT

    Plasmid

    #192262

    Purpose

    AAV9 derived Retrograde vector Optimized for Organ Tracing (ROOT) capsid

    Depositor

    Li Ye

    Article

    Wang et al Nature. 2022 Sep;609(7927):569-574. doi:

    Insert

    Synthetic construct for ROOT VP1 gene
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    p41

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pmScarlet_Giantin_C1

    Plasmid

    #85048

    Purpose

    In vivo visualization of the Golgi apparatus (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    Giantin (GOLGB1 Human)
    

    Expression

    Mammalian

    Mutation

    aa 3131-3259

    Promoter

    CMV

    Available Since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMSCV PIG (Puro IRES GFP empty vector)

    Plasmid

    #21654

    Purpose

    Retroviral backbone for cloning and gene expression. Select with puromycin or GFP.

    Depositor

    David Bartel

    Article

    Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    GFP and IRES

    Expression

    Mammalian

    Available Since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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