We narrowed to 26,847 results for: GFP

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pcDNA5/FRT/TO_eGFP-pScaE

Plasmid

#228826

Purpose

For the expression of the passenger domain of the Orientia tsutsugamushi protein ScaE in HeLa Flp-In cells

Depositor

Andrew Carter

Article

Manigrasso et al bioRxiv 2024.04.11.589026

Insert

ScaE

Tags

eGFP

Expression

Mammalian

Mutation

aa 49-494

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_eGFP-pScaD

Plasmid

#228825

Purpose

For the expression of the passenger domain of the Orientia tsutsugamushi protein ScaD in HeLa Flp-In cells

Depositor

Andrew Carter

Article

Manigrasso et al bioRxiv 2024.04.11.589026

Insert

ScaD

Tags

eGFP

Expression

Mammalian

Mutation

aa 30-700

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_eGFP-pScaC

Plasmid

#228824

Purpose

For the expression of the passenger domain of the Orientia tsutsugamushi protein ScaC in HeLa Flp-In cells

Depositor

Andrew Carter

Article

Manigrasso et al bioRxiv 2024.04.11.589026

Insert

ScaC

Tags

eGFP

Expression

Mammalian

Mutation

aa 33-223

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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FLAG-MKI-P2A-GFP

Plasmid

#229405

Purpose

Lentiviral or overpexression of cDNA

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

MKI-P2A-GFP

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

EFS

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_eGFP-pScaA

Plasmid

#228823

Purpose

For the expression of the passenger domain of the Orientia tsutsugamushi protein ScaA in HeLa Flp-In cells

Depositor

Andrew Carter

Article

Manigrasso et al bioRxiv 2024.04.11.589026

Insert

ScaA

Tags

eGFP

Expression

Mammalian

Mutation

aa 25-1216

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-GfaABC1D-eGFP-2A-mCherry_D4H

Plasmid

#194878

Purpose

Ratiometric D4H sensor for in vivo astrocyte-specific cholesterol visualization

Depositor

Peter Scheiffele

Article

Czernecki et al bioRxiv 2022.11.16.516849

Insert

GFP-T2A-mCherry-D4H

Use

AAV

Expression

Mammalian

Promoter

GfaABC1D

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-GfaABC1D-eGFP-2A-mCherry_YDA

Plasmid

#194879

Purpose

Ratiometric YDA sensor for in vivo astrocyte-specific cholesterol visualization

Depositor

Peter Scheiffele

Article

Czernecki et al bioRxiv 2022.11.16.516849

Insert

GFP-T2A-mCherry-YDA

Use

AAV

Expression

Mammalian

Promoter

GfaABC1D

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-GFP(-7)-neg_tag1

Plasmid

#219419

Purpose

Fluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysate

Depositor

Allie Obermeyer

Article

Ahn et al JACS Au. 2024 Sep 17;4(10):3800-3812. do

Insert

GFP(-7)-neg_tag1

Tags

DEEEDD and MGHHHHHGG

Expression

Bacterial

Promoter

T7

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-3xGFP11-mem-3xPax7gRNA

Plasmid

#224571

Purpose

Ubiquitous expression plasmid with CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three unique Pax7 gRNAs with ribozyme self-cleavage, three membrane split GFP(11) reporter.

Depositor

Erica Hutchins

Article

Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

Insert

3xGFP11

Use

CRISPR

Tags

Membrane Localization Signal

Mutation

Codon optimized

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pMRX-IPU-GFP-Rab5

Plasmid

#228139

Purpose

Expresses GFP-Rab5 in mammalian cells

Depositor

Noboru Mizushima

Article

Kurikawa et al PLoS Biol. 2024 Sep 17;22(9):e3002777. d

Insert

Rab5 (Rab5a Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Available Since

Dec. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pGFP-AUA-IRES-mCherry

Plasmid

#222116

Purpose

Start codon reporter with mutated start codon

Depositor

Jonathan Weissman

Article

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

GFP-IRES-mCherry

Use

Lentiviral

Mutation

AUA start codon

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pGFP-AGC-IRES-mCherry

Plasmid

#222119

Purpose

Start codon reporter with mutated start codon

Depositor

Jonathan Weissman

Article

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

GFP-IRES-mCherry

Use

Lentiviral

Mutation

AGC start codon

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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OSCA1.2-K435L-L438K-peGFP-N1

Plasmid

#228587

Purpose

Expresses OSCA1.2-K435L-L438K-eGFP in mammalian cells

Depositor

Huanghe Yang

Article

Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

Insert

hyperosmolality-gated Ca2+ permeable channel (OSCA) 1.2 (AT4G22120 Mustard Weed)

Expression

Mammalian

Mutation

K435L, L438K

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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SAM-DNMT3A-inactive+sgGFP

Plasmid

#213169

Purpose

Vector with sgGFP used as a negative control (inactive DNMT3A) for induction of global DNA methylation.

Depositor

Sefi Rosenbluh

Article

Hosseinpour et al Nat Commun. 2024 Dec 1;15(1):10449. doi:

Insert

sgGFP

Use

CRISPR, Lentiviral, and Synthetic Biology

Expression

Mammalian

Promoter

E1Fa and U6

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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AICSDP-167:CDH5-mEGFP

Plasmid

#217425

Purpose

Homology arms and mEGFP sequence for C-terminus tagging of human cadherin 5

Depositor

Allen Institute for Cell Science

Insert

CDH5 Homology Arms with mEGFP (CDH5 Human)

Use

CRISPR; Donor template

Tags

mEGFP

Mutation

homology arms contain point mutations to disrupt …

Available Since

Nov. 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pET28a-LSSmGFP-TEV-mScarlet-i

Plasmid

#191199

Purpose

Fusion of LSSmGFP and mScarlet-I separated by a TEV cleavage site for fluorescence-assisted protein purification

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

LSSmGFP & mScarlet-I fusion

Tags

6xHis and mScarlet-I

Expression

Bacterial

Promoter

T7 promoter

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-LSSmGFP-TEV-SAV

Plasmid

#191200

Purpose

Fusion of LSSmGFP and Streptavidin core domain separated by a TEV cleavage site for fluorescence-assisted protein purification

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

LSSmGFP & Streptavidin fusion

Tags

6xHis and Streptavidin core domain, amino acids 1…

Expression

Bacterial

Promoter

T7 promoter

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pLei-sfGFP-V2*D134*Y152*

Plasmid

#191112

Purpose

An E. coli sfGFP reporter with two TAG at positions 2 & 134 & 152

Depositor

Han Xiao

Article

Wu et al Protein Sci. 2022 Oct;31(10):e4443. doi:

Insert

superfolder green fluorescence protein

Tags

His tag

Expression

Bacterial

Mutation

changing V2 & D134 & Y152 to TAG

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2-MfnG-EcYRS-EGFP*

Plasmid

#191119

Purpose

A plasmid for incorporation of O-Me-Tyr into an EGFP reporter without O-Me-Tyr feeding for zebrafish

Depositor

Han Xiao

Article

Wu et al Protein Sci. 2022 Oct;31(10):e4443. doi:

Insert

MfnG - P2A - Mutant E. coli TyrRS - T2A - enhanced green fluorescence protein

Tags

His tag

Expression

Mammalian

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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p50.mALDH1A1.CD63-GFP.wpre

Plasmid

#226770

Purpose

CD63-GFP in p52 (mAldh1a backbone)

Depositor

Ferdinando Pucci

Article

Gonzalez-Callejo et al PLoS One. 2023 Feb 3;18(2):e0279400. doi

Insert

CD63 (CD63 )

Expression

Mammalian

Available Since

Nov. 15, 2024

Availability

Academic Institutions and Nonprofits only

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