We narrowed to 87,364 results for: LAS

1. pcDNA5/FRT/TO(Neo)-Venus-Flag-WT wobbled cdc20 (1991)

   Plasmid

   #61848

   Purpose

   for making stable RPE cells expressing WT cdc20

   Depositor

   Jonathon Pines

   Insert

   cdc20 (CDC20 Human)
   

   Use

   Tags

   Flag and Venus

   Expression

   Mammalian

   Mutation

   wobbled

   Promoter

   CMV

   Available since

   Feb. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pPS311

   Plasmid

   #8903

   Depositor

   Pamela Silver

   Article

   Flach et al Mol Cell Biol. 1994 Dec . 14(12):8399-40

   Insert

   GAL1 promoter (GAL1 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 19, 2005

   Availability

   Academic Institutions and Nonprofits only

3. Δconst-GFP

   Plasmid

   #33114

   Depositor

   Greg Phillips

   Article

   O'Leary et al Mol Biol Cell. 2011 Sep 14.

   Insert

   Pcdh-γA3 (Pcdhga3 Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Deletion of the C-terminal constant region (amino…

   Promoter

   CMV

   Available since

   Oct. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

4. Δ741-752-GFP

   Plasmid

   #33119

   Depositor

   Greg Phillips

   Article

   O'Leary et al Mol Biol Cell. 2011 Sep 14.

   Insert

   Pcdh-γa3 (Pcdhga3 Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Deletion of aa 741-752

   Promoter

   CMV

   Available since

   Oct. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

5. LI B-C N-NLS KOZ c2

   Plasmid

   #54443

   Depositor

   Martin Parniske

   Article

   Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

   Insert

   Nuclear localization signal (NLS) of SV40 large T antigen
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCMV5 T7-Par6 WT T7 tagged

   Plasmid

   #24649

   Depositor

   Jeff Wrana

   Article

   Ozdamar et al Science. 2005 Mar 11. 307(5715):1603-9.

   Insert

   Par6 (Pard6a Mouse)
   

   Use

   Tags

   T7

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 30, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

7. BRPF1 (3MO8)

   Plasmid

   #28107

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   BRPF1:JMC014-E01:C44383 (BRPF1 Human)
   

   Use

   Tags

   His-tag with integrated TEV protease site

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 28, 2011

   Availability

   Industry, Academic Institutions, and Nonprofits

8. pBlue-hFX(I154F)(AA88)-HA

   Plasmid

   #15403

   Depositor

   Michael Ristow

   Insert

   human frataxin cDNA (FXN Human)
   

   Use

   Tags

   AU1, HA, and HIS

   Expression

   Bacterial

   Mutation

   contains cDNA coding sequence for amino acids 88-…

   Promoter

   Available since

   Aug. 17, 2007

   Availability

   Academic Institutions and Nonprofits only

9. LI C-D 1-10GFP noATG

   Plasmid

   #54462

   Depositor

   Martin Parniske

   Article

   Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

   Insert

   superfolder GFP 1-10 domain without start codon
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Mouse Alpha2 delta-2 mutMIDAS HA pMT2

    Plasmid

    #58733

    Purpose

    expression of HA tagged mouse Alpha2 delta-2 with MIDAS site mutations in mammalian cells

    Depositor

    Annette Dolphin

    Insert

    alpha2 delta-2 (Cacna2d2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    MIDAS site mutations (D300VSGS to AVAGA)

    Promoter

    SV40

    Available since

    Oct. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSUPERIOR.puro-shREV3-6

    Plasmid

    #38031

    Depositor

    Emanuela Felley-Bosco, Rolf Stahel

    Article

    Knobel et al Neoplasia. 2011 Oct;13(10):961-70.

    Insert

    shREV3-6 (REV3L Human, Rat, human)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pSUPERIOR.puro-shREV3-5

    Plasmid

    #38030

    Depositor

    Emanuela Felley-Bosco, Rolf Stahel

    Article

    Knobel et al Neoplasia. 2011 Oct;13(10):961-70.

    Insert

    shREV3-5 (REV3L Human, Rat, human)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

13. GRM3 (3SM9)

    Plasmid

    #32870

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    GRM3 (GRM3 Human)
    

    Use

    Tags

    His tag with TEV cleavage and Honeybee melittin S…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 13, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

14. pPS1358

    Plasmid

    #8918

    Depositor

    Pamela Silver

    Article

    Kessler et al Genes Dev. 1997 Oct 1. 11(19):2545-56.

    Insert

    HRP1 (HRP1 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 19, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pASKt15C+SrtA2

    Plasmid

    #64984

    Purpose

    Expresses the E105K/E108A mutant of Staphylococcus aureus sortase A in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/

    Insert

    sortase A
    

    Use

    Tags

    His6 tag and S tag

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-59, E105K, E108A

    Promoter

    tetracycline promoter/operator

    Available since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pASKt15C+SrtAwoH

    Plasmid

    #65017

    Purpose

    Expresses the wild type of Staphylococcus aureus sortase A in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/

    Insert

    sortase A
    

    Use

    Tags

    S tag

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-59

    Promoter

    tetracycline promoter/operator

    Available since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRmHa3-dKDM4A-HA2FL2

    Plasmid

    #20232

    Depositor

    Jerry Workman

    Article

    Lin et al Mol Cell. 2008 Dec 5. 32(5):696-706.

    Insert

    dKDM4A (Kdm4A Fly)
    

    Use

    Tags

    FLAG2 and HA2

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 16, 2009

    Availability

    Academic Institutions and Nonprofits only

18. pGEX-KG-MmUbc6/2790

    Plasmid

    #11491

    Depositor

    Allan Weissman

    Article

    Tiwari et al J Biol Chem. 2001 May 11. 276(19):16193-

    Insert

    MmUbc6 (Ube2j2 Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    n/a

    Promoter

    Available since

    April 5, 2006

    Availability

    Academic Institutions and Nonprofits only

19. pEG608

    Plasmid

    #40194

    Depositor

    Geraldine Seydoux

    Article

    Griffin et al Cell. 2011 Sep 16;146(6):955-68.

    Insert

    Mex-5 (RNAi resistant) (mex-5 Nematode)
    

    Use

    Tags

    3xFLAG (DYKDHDGDYKDHDIDYKDDDDK), GFP, and TEV pro…

    Expression

    Worm

    Mutation

    Exon 2 recoded to be RNAi-resistant

    Promoter

    4.4 kb mex-5 promoter fragment

    Available since

    Sept. 19, 2012

    Availability

    Academic Institutions and Nonprofits only

20. pDNR-hAkt1

    Plasmid

    #49387

    Purpose

    donor vector for cloning human Akt1 with 6xHN tag

    Depositor

    Randall McKinnon

    Insert

    Akt1 (AKT1 Human)
    

    Use

    Cre/Lox; Donor vector

    Tags

    6xHN (6 repeating His-Asn subunits)

    Expression

    Mutation

    tga to cca at nt 1725 with respect to GenBank ID …

    Promoter

    none

    Available since

    Dec. 2, 2013

    Availability

    Academic Institutions and Nonprofits only