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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 18,344 results for: multi

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Showing: 1041 - 1060 of 18344 results

  3. lentiGuide-Crimson

    Plasmid
    #70683
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and Crimson reporter from EF-1a promoter. Lentiviral backbone.
    Depositor
    Daniel Bauer
    Publication
    Canver et al Nature. 2015 Sep 16. doi: 10.1038/nature
    Inserts
    S. pyogenes sgRNA cassette
    Crimson Reporter
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    Ef1-a and hU6
    Available since
    Feb. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    8 Articles
    70683-plasmid-map-sequence-id-217910

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  5. pVITRO1-102.1F10-IgG1/λ

    Plasmid
    #50366
    Purpose
    Expresses grass pollen allergen Phl p 7 specific human IgG1/λ antibody isotype (102.1F10-IgG1/λ)
    Depositor
    Andrew Beavil
    Publication
    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103
    Inserts
    grass pollen allergen Phl p 7 specific human Gamma 1 heavy chain expression cassette
    grass pollen allergen Phl p 7 specific human lambda light chain expression cassette
    Expression
    Mammalian
    Promoter
    Mouse Elongation Factor 1 Alpha and Rat Elongatio…
    Available since
    Jan. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    50366-plasmid-map-sequence-id-313651

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  6. CRY2(L348F)-CreN

    Plasmid
    #75368
    Purpose
    Expresses mCherry tag, then IRES, then Arabidopsis CRY2 (with L348F mutation) fused to N-terminal fragment of Cre DNA recombinase
    Depositor
    Chandra Tucker
    Publication
    Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CRY2 (CRY2 Mustard Weed)
    Tags
    Cre recombinase, residues 19-104
    Expression
    Mammalian
    Mutation
    L348F
    Promoter
    CMV
    Available since
    Aug. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    75368-plasmid-map-sequence-id-142723

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  10. pU6-sgRosa26-1_CBh-Cas9-T2A-BFP-P2A-Ad4E1B

    Plasmid
    #64219
    Purpose
    Expression vector for a sgRNA against the mouse Rosa26 locus and Cas9 linked via T2A to BFP linked to the Ad4 E1B55K gene via P2A
    Depositor
    Ralf Kuehn
    Publication
    Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Cas9
    sgRNA targeting ROSA26-1
    Use
    CRISPR
    Tags
    3xFLAG, NLS, and T2A-BFP-P2A-E1B
    Expression
    Mammalian
    Promoter
    CBh and U6
    Available since
    May 19, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    64219-plasmid-map-sequence-id-107960

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  12. PB_tre_dCas9_VP160

    Plasmid
    #126031
    Purpose
    PiggyBac cargo vector expressing doxycycline inducible dCas9-VP160 fusion
    Depositor
    Mauro Calabrese
    Publication
    Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    dCas9 fusion with VP160
    Hygromycin resistance
    Use
    CRISPR; Piggybac
    Expression
    Mammalian
    Mutation
    D10A, H840A (catalytically inactive Cas9)
    Promoter
    EF1 alpha and TRE
    Available since
    June 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    126031-plasmid-map-sequence-id-246110

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  13. pCSN067

    Plasmid
    #101748
    Purpose
    Single copy yeast plasmid expressing Cpf1 from Lachnospiraceae bacterium ND2006 (LbCpf1), codon optimized for expression in Saccharomyces cerevisiae.
    Depositor
    Rene Verwaal
    Publication
    Verwaal et al Yeast. 2017 Sep 8. doi: 10.1002/yea.3278
    Inserts
    Cpf1 from Lachnospiraceae bacterium ND2006 (LbCpf1) codon optimized for expression in S. cerevisiae.
    KanMX marker expression cassette.
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Yeast
    Promoter
    Heterologous TEF1 promoter from A. gossypii. and …
    Available since
    Dec. 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    5 Articles
    101748-plasmid-map-sequence-id-199897

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  16. pU6-Universal

    Plasmid
    #52694
    Purpose
    This plasmid can be used to mutate genes in Toxoplasma gondii using the CRISPR/Cas9 system after having an appropriate protospacer cloned into it.
    Depositor
    Sebastian Lourido
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Cas9
    Toxoplasma U6 upstream region - protospacer cloning sequence - chiRNA
    Use
    CRISPR; Toxoplasma gondii
    Tags
    3X FLAG and Nuclear localization signal
    Promoter
    TgTUB1 and Toxoplasma U6
    Available since
    June 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    34 Articles
    52694-plasmid-map-sequence-id-82051

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  17. pThermoCas9_ctrl

    Plasmid
    #100981
    Purpose
    Expresses ThermoCas9 and its sgRNA module
    Depositor
    John van der Oost
    Publication
    Mougiakos et al Nat Commun. 2017 Nov 21;8(1):1647. doi:
    Inserts
    Cas9 from the type IIc CRISPR-Cas sytem of Geobacillus thermodenitrificans T12 strain
    ThermoCas9 single guide RNA expressing module
    Tags
    None
    Expression
    Bacterial
    Promoter
    B. coagulans DSM 1 pta promoter and B. smithii xy…
    Available since
    Nov. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100981-plasmid-map-sequence-id-196605

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Showing: 1041 - 1060 of 18344 results