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pMCB306-GFP-Rab29

Plasmid

#198472

Purpose

lentiviral expression of GFP-Rab29

Depositor

Suzanne Pfeffer

Article

Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

Insert

GFP-Rab29 (RAB29 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

EF1A

Available Since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

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pUC57-GFP-SFPQ-RT

Plasmid

#97090

Purpose

Encodes a HR repair template for knock-in of an EGFP insert that fused to the 5'-end of human SFPQ gene, without disturbing its promoter. Best used with px330-GFP-SFPQ vector.

Depositor

Archa Fox

Article

Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

Insert

EGFP-SFPQ-RT (SFPQ Human)

Use

CRISPR

Available Since

July 31, 2017

Availability

Academic Institutions and Nonprofits only

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pAS_FLAG-Mma PylRS

Plasmid

#154762

Purpose

plasmid with Mma PylRS for amber suppression in transient or stable, piggybac-mediated, integration

Depositor

Simon Elsaesser

Article

Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

Insert

Mma PylRS (pylS Methanosarcina mazei)

Tags

FLAG

Expression

Mammalian

Promoter

EF1

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pK7WGF2::hCas9

Plasmid

#46965

Purpose

Expresses the human codon usage Cas9 nuclease (Mali et al. Science 339, 823-826, 2013) with an N-terminal GFP tag from the 35S promoter in the plant tissue

Depositor

Sophien Kamoun

Article

Nekrasov et al Nat Biotechnol. 2013 Aug 8;31(8):691-3.

Insert

hCas9

Use

CRISPR; Plant expression

Tags

eGFP

Promoter

35S

Available Since

Aug. 16, 2013

Availability

Academic Institutions and Nonprofits only

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GST-beta-arrestin1

Plasmid

#36918

Depositor

Robert Lefkowitz

Article

Nobles et al J Biol Chem. 2007 Jul 20;282(29):21370-8

Insert

beta-arrestin1 (Arrb1 Rat)

Tags

GST

Expression

Bacterial

Available Since

June 27, 2012

Availability

Academic Institutions and Nonprofits only
hSTARR-seq_SCP1 vector

Plasmid

#99292

Purpose

Vector to measure enhancer activity of candidates from a DNA library by STARR-seq.

Depositor

Alexander Stark

Article

Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

Type

Empty backbone

Use

Hstarr-seq screening vector

Expression

Mammalian

Available Since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pCRISPRi

Plasmid

#199808

Purpose

vector for constitutive CRISPRi-mediated gene knockdown in S. aureus

Depositor

Steve Salipante

Article

Long et al PLoS Pathog. 2024 Jul 11;20(7):e1012394.

Inserts

dCas9
sgRNA with GFP cassette

Use

CRISPR

Promoter

P23 and PRAB17 (sgRNA) and SarAP1 (GFP)

Available Since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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FUW-H2B-mTurquoise

Plasmid

#193093

Purpose

constitutive expression of H2B-mTurquoise in mammalian cells

Depositor

Filipe Pereira

Article

Silverio-Alves et al Nat Commun. 2023 Aug 14;14(1):4645. doi:

Insert

H2B-mTurquoise

Use

Lentiviral

Expression

Mammalian

Promoter

Ubc

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hAsCpf1(TATV) (pY220)

Plasmid

#89353

Purpose

Expresses humanized AsCpf1 variant that recognizes TATV PAMs

Depositor

Feng Zhang

Article

Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

Insert

Cpf1, RVR variant

Use

CRISPR

Tags

NLS and NLS-3xHA

Expression

Mammalian

Mutation

S542R/K548V/N552R

Promoter

CMV

Available Since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

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pZac2.1 GfaABC1D rM3D mCherry SV40

Plasmid

#92285

Purpose

AAV DREADD expression

Depositor

Baljit Khakh

Article

Chai et al Neuron. 2017 Aug 2;95(3):531-549.e9. doi

Insert

rM3D

Use

AAV

Tags

mCherry

Promoter

GfaABC1D

Available Since

June 26, 2017

Availability

Academic Institutions and Nonprofits only

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pH6HTC_PRDX6-Halo

Plasmid

#175338

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

PRDX6 (PRDX6 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pLyGo-Kp-1

Plasmid

#163143

Purpose

pLyGo cloning vector for a sequence of interest (LPMO) in K. phaffii. Vector encoding the alfa-MF pre-sequence and the LyGo cassette (SapI-ccdB-SapI)

Depositor

Morten Norholm

Article

Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

Type

Empty backbone

Use

Synthetic Biology

Expression

Yeast

Promoter

AOX1

Available Since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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mTFP1-H2A-10

Plasmid

#55488

Purpose

Localization: Nucleus/Histones, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

H2A (H2AC13 Human)

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pLAMP1-miRFP670nano3

Plasmid

#184669

Purpose

LAMP1 (Lysosome Membrane) labelled with near-infrared fluorescent protein miRFP670nano3

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

Insert

LAMP1-miRFP670nano3

Tags

miRFP670nano3

Expression

Mammalian

Promoter

CMV

Available Since

July 25, 2022

Availability

Academic Institutions and Nonprofits only

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SQT1665

Plasmid

#78744

Purpose

Human expression plasmid for LbCpf1-NLS-3xHA

Depositor

Keith Joung

Article

Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

Insert

mammalian codon-optimized Lachnospiraceae bacterium ND2006 Cpf1-NLS-3xHA

Use

CRISPR

Tags

NLS-3xHA

Expression

Mammalian

Promoter

CAG

Available Since

June 16, 2016

Availability

Academic Institutions and Nonprofits only

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Perturb-FISH

Plasmid

#220626

Purpose

expression of gRNAs under U6T7 promoter

Depositor

Samouil Farhi

Article

Binan et al Cell. 2025 Mar 10:S0092-8674(25)00197-7.

Insert

sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

BsmBI

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pCfB2800

Plasmid

#63657

Purpose

pCfB2800 is a vector for multiple integrations at sites sharing homology with Ty2Cons. The selective marker is Kl.LEU2. Additionally it contains a PTEF1-GFP as reporter.

Depositor

Irina Borodina, Jerome Maury

Article

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Insert

GFP

Use

Cre/Lox

Expression

Yeast

Promoter

TEF1 promoter

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-eNme2-C.NR

Plasmid

#185672

Purpose

Expresses nuclease active eNme2-C.NR in mammalian cells

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

Insert

eNme2-C.NR nuclease

Use

CRISPR

Expression

Mammalian

Mutation

eNme2-C S6P/G33E/A520E/S646R/V696F/R711G/V758I/Y7…

Available Since

Sept. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pTDH3-dCas9

Plasmid

#46920

Purpose

Yeast CEN/ARS vector (Leu2) that contains dCas9 fused to NLS controlled by TDH3 promoter

Depositor

Stanley Qi, Jonathan Weissman

Article

Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

Insert

dCas9

Use

CRISPR

Expression

Yeast

Promoter

TDH3

Available Since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pLEX301-TagRFP

Plasmid

#162035

Purpose

Tagged vector control

Depositor

David Bryant

Article

Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

Type

Empty backbone

Use

Lentiviral

Tags

RFP

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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