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K-p35

Plasmid

#38788

Depositor

David Wang

Article

Siebrasse et al J Virol. 2012 Jun 27.

Insert

4385-462 of MWPyV

Expression

Bacterial

Promoter

lac

Available Since

Aug. 21, 2012

Availability

Academic Institutions and Nonprofits only
pSG5-myc-p110α_wt

Plasmid

#39493

Depositor

Julian Downward

Article

Rodriguez-Viciana et al EMBO J. 1996 May 15;15(10):2442-51.

Insert

p110 (PIK3CA Bovine)

Use

Eukaryotic

Tags

myc

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only
pET-21a(+) SurE

Plasmid

#12669

Depositor

SeWon Suh

Article

Lee et al Nat Struct Biol. 2001 Sep . 8(9):789-94.

Insert

SurE (TM1662 T.maritima)

Tags

His

Available Since

Jan. 5, 2007

Availability

Academic Institutions and Nonprofits only
pAAV_EF1a-FLEx(FRT)-GCaMP8m

Plasmid

#252530

Purpose

Expresses the genetically encoded calcium indicator GCaMP8m in Flp-expressing cells under the EF1a promoter for calcium imaging.

Depositor

Gordon Fishell

Article

Mazo et al Res Sq [Preprint]. 2025 Jun 12:rs.3.rs-6

Insert

GCaMP8m

Use

AAV

Expression

Mammalian

Available Since

May 27, 2026

Availability

Academic Institutions and Nonprofits only

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pEvolvR-mCherry-enCas9(D10A)-PolI3M

Plasmid

#249061

Purpose

Expresses nucleus-localized enCas9 (D10A) fused to PolI3M and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.

Depositor

John Dueber, David Schaffer

Article

Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:

Insert

enCas9-PolI3M

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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Vimentin_mCherry_ALFA

Plasmid

#244059

Purpose

anchoring mCherry-1x ALFA to the intermediate filaments

Depositor

Markus Affolter

Article

Vigano et al Development. 2021 Mar 16;148(6):dev19170

Insert

NtVimentin-mCherry-ALFA

Tags

ALFA

Expression

Mammalian

Available Since

Oct. 29, 2025

Availability

Academic Institutions and Nonprofits only

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H2B_mCherry_ALFA

Plasmid

#244047

Purpose

anchoring mCherry-1x ALFAto the chromatin/nucleus

Depositor

Markus Affolter

Article

Vigano et al Development. 2021 Mar 16;148(6):dev19170

Insert

HIST1H2BJ-mCherry-ALFA

Tags

ALFA

Expression

Mammalian

Available Since

Oct. 29, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-HyPerFLEX-NLSx3-WPRE

Plasmid

#217563

Purpose

Mammalian expression of HyPerFLEX targeted to the nucleus

Depositor

Vsevolod Belousov

Article

Potekhina et al Nat Chem Biol. 2025 Oct 16. doi: 10.1038

Insert

HyPerFLEX-NLS

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-ITGAM POD-nAb-WPRE

Plasmid

#244975

Purpose

Expresses a fusion protein of a nanobody against integrin alpha M (ITGAM; CD11b) and peroxidase (POD) in mammalian cells

Depositor

Hiroyuki Hioki

Article

Yamauchi et al Commun Biol. 2025 Jun 18;8(1):903. doi:

Insert

ITGAM POD-nAb

Available Since

Oct. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-RFP6 POD-nAb-WPRE

Plasmid

#244974

Purpose

Expresses a fusion protein of a nanobody against RFP and peroxidase (POD) in mammalian cells

Depositor

Hiroyuki Hioki

Article

Yamauchi et al Commun Biol. 2025 Jun 18;8(1):903. doi:

Insert

RFP6 POD-nAb

Available Since

Oct. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pLEX.(NLS)iGlucoSnFR.H66A.L276V

Plasmid

#238944

Purpose

Green glucose sensor, high sensitivity, nuclear

Depositor

Paul Khavari

Article

Lopez-Pajares et al Cell Stem Cell. 2025 Mar 20:S1934-5909(2

Insert

iGlucoSnFR.L276V.K312A

Use

Lentiviral

Tags

Myc and NLS

Expression

Mammalian

Promoter

CMV

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pUC19_msfGFP-EWSR1-repair-tempate

Plasmid

#237682

Purpose

For knock-in msfGFP to EWSR1 locus

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

EWSR1 (EWSR1 Human)

Use

CRISPR

Tags

msfGFP

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_mEGFP-EWS-FLI1-KS

Plasmid

#237671

Purpose

For overexpression of mEGFP-EWS-FLI1-KS

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

EWS-FLI1-KS (EWSR1 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pMW357

Plasmid

#238204

Purpose

Expresses untagged TaTasR from a PBAD promoter, followed by the native TIGR array (8 units)

Depositor

Feng Zhang

Article

Faure et al Science. 2025 Feb 27:eadv9789. doi: 10.1

Insert

TaTasR

Expression

Bacterial

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pSB700-mouse-TTN-Cerulean

Plasmid

#128325

Purpose

SP-dCas9 compatible gRNA to be used as a positive control for activation experiments (mouse cells)

Depositor

Alejandro Chavez

Insert

gRNA against mouse TTN for activation

Expression

Mammalian

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-mCAMSAP3-Myc

Plasmid

#236536

Purpose

For expression of CAMSAP3 (mouse) in mammalian cells

Depositor

Sandra Citi

Article

Flinois et al MicroPubl Biol. 2024 Oct 14;2024:10.1791

Insert

CAMSAP3 (Camsap3 Mouse)

Tags

GFP and Myc

Expression

Mammalian

Mutation

WT

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pgRNA-R-gfp

Plasmid

#220927

Purpose

Expresses gfp using J23119 promoter and pgRNA_AT backbone

Depositor

Andrea Puhar

Article

Sharma et al mSystems. 2022 Dec 21:e0104522. doi: 10.

Insert

superfolder GFP

Tags

FLAG

Expression

Bacterial

Promoter

J23119

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pMGA-plux-sfGFP

Plasmid

#139936

Purpose

AHL-inducible sfGFP expression on M. magneticum/E. coli shuttle vector

Depositor

Christopher Voigt

Article

Furubayashi et al Adv. Funct. Mater. 2021, 31, 2004813

Insert

LuxR-Plux-sfGFP

Expression

Bacterial

Available Since

July 4, 2024

Availability

Academic Institutions and Nonprofits only

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sgRNA scaffold-spacer-human U6

Plasmid

#214697

Purpose

provide "sgRNA scaffold-spacer-human U6" fragment

Depositor

Yin Shen

Article

Ren et al Mol Cell. 2023 Dec 21;83(24):4633-4645.e

Insert

sgRNA scaffold-spacer-human U6

Use

CRISPR

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pCHA1.1-Rap2a-V5-Hygro

Plasmid

#195510

Purpose

lentiviral vector expressing RAP2A CDS with hygromycin selection

Depositor

Joanna Wysocka

Article

Bajpai et al Science. 2023 Aug 11;381(6658):eade6289.

Insert

Hygromycin

Use

Lentiviral

Expression

Mammalian

Promoter

hPGK

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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