We narrowed to 12,148 results for: NSI;
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Plasmid#22282PurposeExpresses PhyB-mCFP with prenylation sequence from Kras, for red-light-induced recruitment of PIF to the plasma membraneDepositorInsertpCMV-PhyB(1-908)-10aaLinker-mCFP-Kras4BCT (PHYB Mustard Weed)
UseSynthetic BiologyExpressionMammalianAvailable SinceFeb. 19, 2010AvailabilityAcademic Institutions and Nonprofits only -
pHR SFFVp BFP-Erk2
Plasmid#50848PurposeFluorescent reporter for Erk nuclear translocationDepositorAvailable SinceJan. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-DIO(ChRger2-TS-YFP)
Plasmid#127239PurposeHigh photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CAG promoter and double floxed.DepositorInsertChRger2-TS-EYFP
UseAAVTagsEYFP and SpyTagExpressionMammalianPromoterCAGAvailable SinceSept. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTorPE-R-GECO1
Plasmid#32465DepositorInsertR-GECO1.0
UseLab constructedTags6x His and Modified TorA MNNNDLFQASRRRFLAQLG[G to…ExpressionBacterialMutationSubstitutions relative to the mApple-derived anal…Available SinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCAMBIA-FLS2-myc-mCherry
Plasmid#86159PurposeFLS2-mCherry expressionDepositorAvailable SinceFeb. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti HRE mutant-Luc pGK Hygro
Plasmid#118707Purposereporter plasmid, HIF-responsive promoter (3xHRE) mutant- fused to firefly luciferaseDepositorInsertHREmutant-Luc
UseLentiviralAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
ZIKV_Sensor_27B_LacZ
Plasmid#75006PurposeToehold switch sensor to detect Zika VirusDepositorInsertZIKV_sensor_27B_LacZ
TagsLacZExpressionBacterialPromoterT7Available SinceDec. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1 3xFlag-CREB
Plasmid#82721PurposeExpresses human CREBDepositorAvailable SinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
α-actinin-4-M522-sstFRET(Venus/Cerulean) in pUNO1
Plasmid#190799PurposeImaging of force-sensitive α-actinin-4 in live cellsDepositorInsertα-actinin-4-Venus-Cerulean-522 (ACTN4 Human, Synthetic)
Tagscerulean and venusExpressionMammalianPromoterhEF1-HTLVAvailable SinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMD19_STARR-seq_plants
Plasmid#117379PurposeSTARR-seq vector with 35s core promoter for transient expression in plant protoplastsDepositorTypeEmpty backboneTagsGFP derived from pMDC107ExpressionPlantPromoter35s core promoterAvailable SinceNov. 22, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
PpyCAG-KLF2-IB
Plasmid#60441PurposeTransient human KLF2 expressionDepositorInsertKLF2 (KLF2 Human)
ExpressionMammalianAvailable SinceSept. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
RDX-mCherry
Plasmid#214476PurposePlasmid for transient transfection of RDX-mCherry fusion protein in mammalian cells.DepositorInsertRDX (RDX Human)
TagsmCherryExpressionMammalianMutationSequence matches cDNA clone MGC:48283 IMAGE:52844…PromoterCMVAvailable SinceMarch 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
HSC1-HS4-GiP
Plasmid#58540PurposeDerived from HSC1-GiP where 2 copies of the HS4 insulator core are cloned inside the 3'LTRDepositorInsertsEnhanced Green Fluorescence Protein, Puromycin resistance gene
Core HS4 insulator sequence (dimer)
UseRetroviralExpressionMammalianPromoterHuman EF1-alphaAvailable SinceSept. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pX335-EN475
Plasmid#86231PurposeFor transient expression of spCas9-nickase and one sgRNA targeting the mouse CTCF locus. Use together with pX335-EN477.DepositorInsertspCas9-nickase and sgRNA against mouse CTCF STOP Codon
UseMouse TargetingExpressionMammalianAvailable SinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pX335-EN477
Plasmid#86232PurposeFor transient expression of spCas9-nickase and one sgRNA targeting the mouse CTCF locus. Use together with pX335-EN475.DepositorInsertspCas9-nickase and sgRNA against mouse CTCF STOP Codon
UseMouse TargetingExpressionMammalianAvailable SinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
Biomolecular FeedBack (FB)
Plasmid#109391PurposeThis construct encodes for two units. The first contains a constitutively expressed dCas9 protein. The second contains an sgRNA cassette under the control of the burden-responsive htpG1 promoter.DepositorInsertpJ23100mut-dCas9-T- phtpG1-sgRNA
ExpressionBacterialAvailable SinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
mRFP-mem
Plasmid#55622PurposeThis red fluorescent plasma membrane marker consists of the first twenty residues of neuromodulin fused to mRFP.DepositorInsertmRFP-Mem
TagsThe N-terminal 20 residues of neuromodulin are fu…ExpressionMammalianPromoterCMVAvailable SinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
PpyCAG-NANOG-IB
Plasmid#60440PurposeTransient human NANOG expressionDepositorInsertNANOG (NANOG Human)
ExpressionMammalianAvailable SinceSept. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
p-mCherry-C1-mJP-4
Plasmid#79599PurposeTransient expressionDepositorAvailable SinceJuly 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
CoV2-N-P.1
Plasmid#177959PurposeTransient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (P80R, R203K, G204R). Used for generating RNA packaging virus-like particles.DepositorInsertSARS-CoV-2 Nucleocapsid (P80R, R203K, G204R) (N )
ExpressionMammalianMutationP80R,R203K,G204RAvailable SinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only