We narrowed to 3,025 results for: GRI;

1. pcDNA3.1(+)_Nav1.6(WT, Y371C)-P2A-eGFP

   Plasmid

   #239768

   Purpose

   Expresses independantly TTX-resistant (Y371C) variant of mouse Nav1.6WT and eGFP in mammalian cells.

   Depositor

   Ivana Nikić-Spiegel

   Article

   Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060

   Insert

   Scn8a (Scn8a Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Y371C

   Promoter

   CMV

   Available since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLentiCRISPR V2 Neo PTEN sgRNA5

   Plasmid

   #233246

   Purpose

   Knock out of murine PTEN

   Depositor

   David Bryant

   Article

   Nikolatou et al EMBO J. 2023 Sep 18;42(18):e113987. doi:

   Insert

   PTEN (Pten Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLentiCRISPR V2 Neo sgITGA5_5

   Plasmid

   #233253

   Purpose

   Knock out of murine ITGA5

   Depositor

   David Bryant

   Article

   Nikolatou et al EMBO J. 2023 Sep 18;42(18):e113987. doi:

   Insert

   ITGA5 (Itga5 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLentiCRISPR V2 Neo sgITGA5_4

   Plasmid

   #233252

   Purpose

   Knock out of murine ITGA5

   Depositor

   David Bryant

   Article

   Nikolatou et al EMBO J. 2023 Sep 18;42(18):e113987. doi:

   Insert

   ITGA5 (Itga5 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLentiCRISPR V2 Neo sgITGA5_1

   Plasmid

   #233251

   Purpose

   Knock out of murine ITGA5

   Depositor

   David Bryant

   Article

   Nikolatou et al EMBO J. 2023 Sep 18;42(18):e113987. doi:

   Insert

   ITGA5 (Itga5 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLentiCRISPR V2 Neo sgAGAP1_3

   Plasmid

   #233248

   Purpose

   Knock out of murine AGAP1

   Depositor

   David Bryant

   Article

   Nikolatou et al EMBO J. 2023 Sep 18;42(18):e113987. doi:

   Insert

   AGAP1 (Agap1 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLentiCRISPR V2 Neo PTEN sgRNA2

   Plasmid

   #233245

   Purpose

   Knock out of murine PTEN

   Depositor

   David Bryant

   Article

   Nikolatou et al EMBO J. 2023 Sep 18;42(18):e113987. doi:

   Insert

   PTEN (Pten Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLentiCRISPR V2 Neo sgAGAP1_2

   Plasmid

   #233247

   Purpose

   Knock out of murine AGAP1

   Depositor

   David Bryant

   Article

   Nikolatou et al EMBO J. 2023 Sep 18;42(18):e113987. doi:

   Insert

   AGAP1 (Agap1 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Rab11 single guide RNA

   Plasmid

   #229683

   Purpose

   guide RNA for Rab11 tagging at the N-terminus in pX459

   Depositor

   Francesca Bottanelli

   Article

   Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/

   Insert

   Rab11 guide (RAB11A Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pX330-U6-(sgRNA-T2)-Chimeric_BB-CBh-hSpCas9

    Plasmid

    #223323

    Purpose

    Human codon optimized Cas9 expressing plasmid that carries the sgRNA T2 from Mali et al., 2013 (10.1126/science.1232033).

    Depositor

    Jonathan Arias

    Article

    Petruskeviciute et al Stem Cell Res. 2024 Dec 12;82:103628. do

    Insert

    SpCas9 and sgRNA-T2 (cas9 )
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. LMPd Amt PHGDH

    Plasmid

    #209409

    Purpose

    Retroviral vector with Ametrine marker for expressing shRNA with an "UltramiR" microRNA scaffold

    Depositor

    Russell Jones

    Article

    Ma et al Immunity. 2019 Nov 19;51(5):856-870.e5.

    Insert

    Phgdh shRNA (Phgdh Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pITGA5_1745G>U

    Plasmid

    #173812

    Purpose

    mir218 binding site2 point mutation

    Depositor

    Andrew M. Siedlecki

    Article

    Liu et al J Am Soc Nephrol. 2022 Mar;33(3):565-582

    Insert

    NT1745 ITGA5 point mutation (ITGA5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    AmpR

    Available since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pITGA5_473G>A

    Plasmid

    #173811

    Purpose

    mir218 binding site1 point mutation

    Depositor

    Andrew M. Siedlecki

    Article

    Liu et al J Am Soc Nephrol. 2022 Mar;33(3):565-582

    Insert

    NT473 ITGA5 point mutation (ITGA5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    AmpR

    Available since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pITGA5_Q853L

    Plasmid

    #174008

    Purpose

    Fibronectin receptor binding site point mutation

    Depositor

    Andrew M. Siedlecki

    Article

    Liu et al J Am Soc Nephrol. 2022 Mar;33(3):565-582

    Insert

    FibronR ITGA5 point mutation (ITGA5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    AmpR

    Available since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMXS-IRES-Blast POLA1 ISCmut

    Plasmid

    #160808

    Purpose

    Express ISC mutant POLA1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    POLA1 ISCmut (POLA1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Codon optimized, C1354S, C1359S, C1377S, C1380S

    Promoter

    Available since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. AAV2_hSyn_Aurora_CA_Citrine

    Plasmid

    #98219

    Purpose

    Slow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with green light, inactivation max with 625 nm (accelarates closure to ms). Not recommended for in vivo use. Codon optimized for mammalian expression.

    Depositor

    Simon Wiegert

    Article

    Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

    Insert

    Synthetic construct Aurora_C128A gene
    

    Use

    AAV

    Tags

    Citrine

    Expression

    Mammalian

    Mutation

    V59S, E83N, E90Q, E101S, V117R, E123S, C128A, P24…

    Promoter

    human synapsin

    Available since

    Aug. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3-CARD14-L156P

    Plasmid

    #69918

    Purpose

    Expresses mutant FLAG-CARD14 in mammalian cells

    Depositor

    Francesca Capon

    Article

    Berki et al J Invest Dermatol. 2015 Jul 23. doi: 10.

    Insert

    CARD14 isoform 3 (CARD14 Human)
    

    Use

    Tags

    FLAG

    Expression

    Bacterial and Mammalian

    Mutation

    L156P

    Promoter

    CMV

    Available since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

18. loxP-Hygro-loxP-HA-mStayGold-Rab11 HR

    Plasmid

    #229678

    Purpose

    Homology repair plasmid for endogenous tagging of Rab11 at the N-terminus with monomeric StayGold and an HA epitope tag. Contains a hygromycin resistance cassette for selection of edited cells

    Depositor

    Francesca Bottanelli

    Article

    Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/

    Insert

    genomic sequence Rab11 locus (RAB11A Human)
    

    Use

    CRISPR

    Tags

    monomeric StayGold, HA

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AP1muA-mStayGold-polyA-Hygro HR

    Plasmid

    #229679

    Purpose

    Homology repair plasmid for endogenous tagging of AP1muA at the C-terminus with monomeric StayGold. Contains a hygromycin resistance cassette for selection of edited cells

    Depositor

    Francesca Bottanelli

    Article

    Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/

    Insert

    genomic sequence AP1M1 locus (AP1M1 Human)
    

    Use

    CRISPR

    Tags

    monomeric StayGold

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAGGS-ACE2-his expression plasmid

    Plasmid

    #158089

    Purpose

    Expresses ACE2 in mammalian cells

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

    Insert

    ACE2 (ACE2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    See depositor comments below

    Promoter

    Available since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. IST1-GFP

    Plasmid

    #186298

    Purpose

    Expresses human IST1 C-terminally fused to GFP

    Depositor

    Geert van den Bogaart

    Article

    Stempels et al J Cell Biol. 2023 Jul 3;222(7):e20220513

    Insert

    IST1 homolog (IST1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    GFP

    Promoter

    CMV

    Available since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pKLV2-EF1a-Cas9Bsd-W

    Plasmid

    #68343

    Purpose

    Lentiviral vector expressing Cas9 fused with the Blasticidin resistant gene at the C-terminus

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    EF1a-Cas9 cassette, WPRE
    

    Use

    CRISPR and Lentiviral

    Tags

    Cas9 fused with Flag-tag and a NLS at the N termi…

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pKLV2-U6gRNA5(gBFP)-PGKmCherry2ABFP-W

    Plasmid

    #67986

    Purpose

    Cas9 activity reporter with mCherry and BFP

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Inserts

    U6gRNA cassette, PGKmCherryABFP cassette, WPRE
    Guide RNA targeting modified BFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Deleted BbsI site within WPRE, modified BFP

    Promoter

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP-W

    Plasmid

    #67974

    Purpose

    CRISPR gRNA expression vector with an improved scaffold and puro/BFP markers

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette, PGKpuro2ABFP cassette, WPRE
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Deleted BbsI site within WPRE

    Promoter

    human U6 promoter, mouse PGK promoter

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pKLV2-U6gRNA5(Empty)-PGKBFP2AGFP-W

    Plasmid

    #67979

    Purpose

    Cas9 activity reporter (control) with BFP and GFP.

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette, PGKBFP2AGFP cassette, WPRE
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Deleted BbsI site within WPRE

    Promoter

    human U6 promoter, mouse PGK promoter

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGP-pcDNA3.1 Puro-CAG-Voltron2

    Plasmid

    #172909

    Purpose

    Mammalian expression of voltage sensor

    Depositor

    GENIE Project

    Article

    Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-

    Insert

    Voltron2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A122D

    Promoter

    CAG

    Available since

    Nov. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pKLV2-U6gRNA5(BbsI)-PGKpuro2AmCherry-W

    Plasmid

    #67977

    Purpose

    CRISPR gRNA expression vector with an improved scaffold and puro/mCherry markers

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette, PGKpuro2AmCherry cassette, WPRE
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Deleted BbsI site within WPRE

    Promoter

    human U6 promoter, mouse PGK promoter

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP

    Plasmid

    #67991

    Purpose

    CRISPR gRNA expression vector with an improved l scaffold and puro/BFP markers without WPRE

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette with an improved scaffold, PGKpuro2ABFP cassette
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA3.0-Magneto2.0-p2A-mCherry

    Plasmid

    #74308

    Purpose

    Bicistronic expression of Magneto2.0 and mCherry in mammalian cells

    Depositor

    Ali Guler

    Article

    Wheeler et al Nat Neurosci. 2016 Mar 7. doi: 10.1038/n

    Insert

    Magneto2.0 (FTL, FTH1, Trpv4 Rat, Human)
    

    Use

    Tags

    Flag and p2A-mCherry

    Expression

    Mammalian

    Mutation

    TRPV4: delta 760–871

    Promoter

    CMV

    Available since

    May 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAAV-pBG-mCRE98-NLS-EGFP-WPRE

    Plasmid

    #239977

    Purpose

    adeno-associated virus (AAV) encoding enhanced green fluorescent protein (eGFP) under motor neuron-selective control of minimal beta globin promoter (pBG) and CRE98

    Depositor

    Sinisa Hrvatin

    Article

    Nagy et al Proc Natl Acad Sci U S A. 2024 Dec 3;121

    Insert

    EGFP (eGFP Synthetic)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    pBG + mCRE98

    Available since

    June 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pKLV2.2-h7SKgRNA5(SapI)-hU6gRNA5(BbsI)-PGKpuroBFP-W

    Plasmid

    #72666

    Purpose

    Lentiviral dual CRISPR gRNA expression vector (human 7SK and human U6 promoters)

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    h7SKgRNA cassette, hU6gRNA cassette, PGKpuroBFP cassette, WPRE
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. plenti-FLAG-CAMK2D

    Plasmid

    #221699

    Purpose

    Lentiviral expression of FLAG-tagged human CAMK2D in mammalian cells

    Depositor

    Derrick Sek Tong Ong

    Article

    Chuah et al Cell Death Differ. 2023 Aug;30(8):1973-1

    Insert

    CAMK2D (CAMK2D Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. anti-Desmin_D7-pBIOCAM5

    Plasmid

    #39346

    Depositor

    John McCafferty

    Article

    Schofield et al Genome Biol. 2007;8(11):R254.

    Insert

    scFv-Fc-fusion specific for Desmin (clone D7) (DES Human)
    

    Use

    Tags

    3xFLAG, 6xHis, and human Fc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pNeg-Ma-barnase-TAG3-TAG45

    Plasmid

    #197572

    Purpose

    Negative selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses 2xTAG-codon interrupted barnase gene and M. alvus Pyl-tRNA(6). p15a origin of replication.

    Depositor

    Ryan Mehl

    Article

    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

    Inserts

    Barnase - 2xTAG
    M. alvus Pyl-tRNA (6)
    

    Use

    Tags

    none

    Expression

    Bacterial

    Mutation

    3TAG and 45TAG

    Promoter

    araC and lpp

    Available since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pPos-Ma-CmR-TAG111

    Plasmid

    #197571

    Purpose

    Positive selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses TAG-codon interrupted chloramphenicol resistance cassette and M. alvus Pyl-tRNA(6). p15a ori

    Depositor

    Ryan Mehl

    Article

    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

    Inserts

    Chloramphenicol resistance gene - 111TAG
    T7 polymerase -TAG
    GFPuv
    M. alvus Pyl-tRNA(6)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    111TAG and 8TAG/112TAG

    Promoter

    AraC, T7, cat promoter (constitutive), and lpp (c…

    Available since

    April 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. plenti-FLAG-CAMK2D-ED

    Plasmid

    #221700

    Purpose

    Lentiviral expression of FLAG-tagged human CAMK2D (K43R/D136N) in mammalian cells

    Depositor

    Derrick Sek Tong Ong

    Article

    Chuah et al Cell Death Differ. 2023 Aug;30(8):1973-1

    Insert

    CAMK2D (CAMK2D Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    K43R, D136N

    Promoter

    Available since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pscALPSpuro-RsACE2

    Plasmid

    #159665

    Purpose

    Expresses Rhinolophus sinicus ACE2 in mammalian cells

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

    Insert

    rhinolophus sinicus ACE2 (ACE2 rhinolophus sinicus)
    

    Use

    Lentiviral

    Tags

    Myc tag

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pKLV2-U6gRNA5(gGFP)-PGKmCherry2AGFP-W

    Plasmid

    #67982

    Purpose

    Cas9 activity reporter with mCherry and GFP

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette, PGKmCherry2AGFP cassette, WPRE
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Deleted BbsI site within WPRE

    Promoter

    Available since

    Nov. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pLVET-HA-K-RasG12V-IRES-GFP

    Plasmid

    #107140

    Purpose

    A bicistronic lentiviral mammalian expression vector for HA-KRasG12V(4B) and GFP

    Depositor

    Aki Manninen

    Article

    Zhang et al Oncogene. 2017 Oct 12;36(41):5681-5694.

    Insert

    HA-tagged Kras4B(G12V) and GFP (KRAS Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hEF-1alpha

    Available since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pKLV2-U6gRNA5(Empty)-PGKmCherry2ABFP-W

    Plasmid

    #67985

    Purpose

    Cas9 activity reporter (control) with mCherry and BFP

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette, PGKmCherryABFP cassette, WPRE
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Deleted BbsI site within WPRE, modified BFP

    Promoter

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. loxP-G418-loxP-3xALFA-Halo-Rab11 HR

    Plasmid

    #229676

    Purpose

    Homology repair plasmid for endogenous tagging of Rab11 at the N-terminus with Halotag and 3x ALFA tag. Contains a G418 resistance cassette for selection of edited cells

    Depositor

    Francesca Bottanelli

    Article

    Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/

    Insert

    genomic sequence Rab11 locus (RAB11A Human)
    

    Use

    CRISPR

    Tags

    Halo, 3xALFA

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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42. pKLV2.2-mU6gRNA5(SapI)-hU6gRNA5(BbsI)-PGKpuroBFP-W

    Plasmid

    #72667

    Purpose

    Lentiviral dual CRISPR gRNA expression vector (mouse U6 and human U6 promoters)

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    mU6gRNA cassette, hU6gRNA cassette, PGKpuroBFP cassette, WPRE
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pEGFP-dnMCAK

    Plasmid

    #208650

    Purpose

    Mammalian expression plasmid expressing eGFP-dnMCAK driven by CMV promoter

    Depositor

    Samuel Bakhoum

    Article

    Li et al Nature. 2023 Aug;620(7976):1080-1088. do

    Insert

    Kif2c (Kif2c Cricetulus griseus (Chinese hamster))
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    S92A , S106A , S108A, S112A , S186A, H530A, R534A…

    Promoter

    Available since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pET21a(+)-HIV RT-6xHis-RBS-prot

    Plasmid

    #159149

    Purpose

    expresses His-tagged HIV reverse transcriptase and untagged HIV protease

    Depositor

    Andrea Pauli

    Article

    Kellner et al Front Mol Biosci. 2022 Apr 1;9:801309. d

    Inserts

    Human immunodeficiency virus 1 (HIV-1) reverse transcriptase
    Human immunodeficiency virus 1 (HIV-1) protease
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    HIV-1 group M/subtype B – BH10 strain

    Promoter

    Available since

    Sept. 1, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

45. pKLV2-EF1a-BsdCas9-W

    Plasmid

    #67978

    Purpose

    Lentiviral vector expressing Cas9 fused with the Blasticidin resistant gene at the N-terminus

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    EF1a-Cas9 cassette, WPRE
    

    Use

    CRISPR and Lentiviral

    Tags

    Cas9 fused with Flag-tag and a NLS at the N termi…

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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46. pFastBac Dual wt p110α/p85α

    Plasmid

    #199302

    Purpose

    co-expression of His-tagged p110α wt and Avi-tagged p85α wt proteins

    Depositor

    Peter Vogt

    Article

    Favalli et al Nat Chem. 2021 Jul;13(7):714. doi: 10.10

    Insert

    His-tagged p110α wt and Avi-tagged p85α wt (PIK3CA, PIK3R1 Human)
    

    Use

    Tags

    Avi and His

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. loxP-Puro-loxP-3xV5-SNAP-Rab11 HR

    Plasmid

    #229677

    Purpose

    Homology repair plasmid for endogenous tagging of Rab11 at the N-terminus with SNAP tag and a 3x V5 epitope tag. Contains a puromycin resistance cassette for selection of edited cells.

    Depositor

    Francesca Bottanelli

    Article

    Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/

    Insert

    genomic sequence Rab11 locus (RAB11A Human)
    

    Use

    CRISPR

    Tags

    SNAP, 3xV5

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pKLV2-U6gRNA5(BbsI)-PGKpuro2AZsG-W

    Plasmid

    #67975

    Purpose

    CRISPR gRNA expression vector with an improved scaffold and puro/ZsG markers

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette, PGKpuro2AZsG cassette, WPRE
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Deleted BbsI site within WPRE

    Promoter

    human U6 promoter, mouse PGK promoter

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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49. COVID-SARS2 NSP14/10

    Plasmid

    #159613

    Purpose

    Bacterial co-expression vector fo Covid-SARS2 NSP14 and NSP10

    Depositor

    Opher Gileadi

    Article

    Baddock et al Nucleic Acids Res. 2022 Jan 17. pii: 650

    Inserts

    Covid-SARS2 Nsp14 (ORF1ab COVID-SARS2, Synthetic)
    Covid-SARS2 Nsp10
    

    Use

    Tags

    His6, TEV cleavage site and none

    Expression

    Bacterial

    Mutation

    Codon-optimized for E. coli expression

    Promoter

    (Bicistronic) and T7 - LacO

    Available since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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50. pAAV-CAG-FLEX-SomaGCaMP7

    Plasmid

    #182942

    Purpose

    Cre-dependent expression of cell body-targeted GCaMP7f, under CAG promoter. As bright as conventional GCaMP7f.

    Depositor

    Edward Boyden

    Article

    Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)

    Insert

    Soma-GCaMP7
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge