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We narrowed to 15,063 results for: pET

Showing: 2651 - 2700 of 15063 results

  2. pET21-pelB-LaG-3

    Plasmid
    #172740
    Purpose
    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-3; HIS tag preceded by HRV 3C/PreScission cleavage site.
    Depositor
    Michael Rout
    Article
    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do
    Insert
    LaG-3
    Use
    Tags
    6xHIS and pelB leader for periplasmic secretion
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    Aug. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    172740-plasmid-map-sequence-id-340191

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  6. pET-DEST42-dre4

    Plasmid
    #170440
    Purpose
    Express drosophila dre4 in E.coli expression system. Generated from pDONR-dre4 by Gateway system. Used for custom antibody purification.
    Depositor
    Joan Conaway
    Article
    Tettey et al Cell Rep. 2019 Jun 25;27(13):3770-3779.e
    Insert
    dre4 (dre4 Fly)
    Use
    Tags
    6xHis and V5
    Expression
    Bacterial
    Mutation
    Deleted amino acids 1-20
    Promoter
    T7
    Available Since
    July 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    170440-plasmid-map-sequence-id-338015

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  29. pET11b-MIF H62F

    Plasmid
    #149445
    Purpose
    Expression of MIF H62F in E. coli for protein production
    Depositor
    Elias Lolis
    Insert
    MIF (MIF Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    H62F
    Promoter
    Available Since
    Sept. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    149445-plasmid-map-sequence-id-311584

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  30. pET11b-MIF H62A

    Plasmid
    #149443
    Purpose
    Expression of MIF H62A in E. coli for protein production
    Depositor
    Elias Lolis
    Insert
    MIF (MIF Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    H62A
    Promoter
    Available Since
    Sept. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    149443-plasmid-map-sequence-id-311582

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  31. pET11b-MIF H62Y

    Plasmid
    #149442
    Purpose
    Expression of MIF H62Y in E. coli for protein production
    Depositor
    Elias Lolis
    Insert
    MIF (MIF Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    H62Y
    Promoter
    Available Since
    Sept. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    149442-plasmid-map-sequence-id-311581

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  32. pET11b-MIF Y99G

    Plasmid
    #149441
    Purpose
    Expression of MIF Y99G in E. coli for protein production
    Depositor
    Elias Lolis
    Insert
    MIF (MIF Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Y99G
    Promoter
    Available Since
    Sept. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    149441-plasmid-map-sequence-id-311580

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  34. pSHDY*in_PpetE:BCD2:AgBIS:Flag

    Plasmid
    #133971
    Purpose
    Heterologous, copper-inducible expression of (E)-α-bisabolene synthase from Abies grandis (AgBIS) in Synechocystis sp. PCC 6803. The AgBIS CDS is fused to a C-termial Flag-tag.
    Depositor
    Pia Lindberg
    Article
    Dienst et al Sci Rep. 2020 Apr 3;10(1):5932. doi: 10.
    Insert
    AgBIS:Flag
    Use
    Synthetic Biology
    Tags
    Flag
    Expression
    Bacterial
    Mutation
    inserted gene is codon optimized for Synechocyst…
    Promoter
    PpetE:BCD2
    Available Since
    April 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    133971-plasmid-map-sequence-id-271350

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  35. pSHDY*in_PpetE:BCD2:MrBBS:Strep

    Plasmid
    #133973
    Purpose
    Heterologous, copper-inducible expression of (-)-α- bisabolol synthase from Matricaria recutita (MrBBS) in Synechocystis sp. PCC 6803. The MrBBS CDS is fused to a C-termial StrepII-tag.
    Depositor
    Pia Lindberg
    Article
    Dienst et al Sci Rep. 2020 Apr 3;10(1):5932. doi: 10.
    Insert
    MrBBS:StrepII
    Use
    Synthetic Biology
    Tags
    StrepII
    Expression
    Bacterial
    Mutation
    inserted gene is codon optimized for Synechocyst…
    Promoter
    PpetE:BCD2
    Available Since
    April 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    133973-plasmid-map-sequence-id-267788

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  36. pET-FLAG-B-eSpCas9

    Plasmid
    #126772
    Purpose
    Expression of increased fidelity Blackjack-eSpCas9 in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than that of eSpCas9
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    B-eSpCas9
    Use
    CRISPR
    Tags
    3xFLAG, 6xHis, MBP, and NLS
    Expression
    Bacterial
    Mutation
    K848A; K1003A; R1060A; amino acids 1005-1013 repl…
    Promoter
    T7
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126772-plasmid-map-sequence-id-410600

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  42. pET14b-dabAB2 D353A

    Plasmid
    #133016
    Purpose
    pET14b carrying the dabA2 gene (Uniprot: D0KWS7) with a D353A mutation fused to a c-terminal strep tag and the dabB2 gene (Uniprot: D0KWS8) with a c-terminal sfGFP V206K fusion and 6xHis tag
    Depositor
    David Savage
    Article
    Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038
    Inserts
    dabB2
    dabA2
    Use
    Tags
    6xHis, StrepII tag, and sfGFP
    Expression
    Bacterial
    Mutation
    D353A
    Promoter
    t7
    Available Since
    Nov. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    133016-plasmid-map-sequence-id-262984

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  43. pET14b-dabAB2 C351A

    Plasmid
    #133014
    Purpose
    pET14b carrying the dabA2 gene (Uniprot: D0KWS7)  with a C351A mutation fused to a c-terminal strep tag and the dabB2 gene (Uniprot: D0KWS8) with a c-terminal sfGFP V206K fusion and 6xHis tag
    Depositor
    David Savage
    Article
    Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038
    Inserts
    dabB2
    dabA2
    Use
    Tags
    6xHis, StrepII tag, and sfGFP
    Expression
    Bacterial
    Mutation
    C351A
    Promoter
    t7
    Available Since
    Nov. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    133014-plasmid-map-sequence-id-262980

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Showing: 2651 - 2700 of 15063 results