We narrowed to 34,841 results for: CaS;

1. ACE2 g4 + Cas9

   Plasmid

   #153014

   Purpose

   A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing Cas9 and TagBFP2

   Depositor

   Jason Sheltzer

   Insert

   Cas9 + ACE2 gRNA (ACE2 Human)
   

   Use

   Lentiviral

   Tags

   TagBFP2

   Expression

   Mutation

   Promoter

   Available since

   June 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-T3-eSpCAS9-NG-pA

   Plasmid

   #131466

   Purpose

   Expresses eSpCAS9-NG nuclease in mammalian cells. Used to modify genome of mouse embroys

   Depositor

   Wataru Fujii

   Article

   Fujii et al Sci Rep. 2019 Sep 9;9(1):12878. doi: 10.

   Insert

   Codon optimized eSpCas9-NG
   

   Use

   CRISPR

   Tags

   Flag, NLS, and polyA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. PRE-Hsp70BbCas9_1.2

   Plasmid

   #190796

   Purpose

   gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP; heat-shock inducible Cas9-T2A-eGFP expression regulated by upstream Polycomb response elements with one gypsy insulator element. Step 2 in cloning process.

   Depositor

   Omar Akbari

   Article

   Warsinger-Pepe et al G3 (Bethesda). 2023 Apr 11;13(4):jkad024

   Insert

   gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAGM4723:Ble:Cas9

   Plasmid

   #112208

   Purpose

   Golden Gate Level 2 construct encoding Cas9-YFP, Zeocin resistance.

   Depositor

   Thomas Mock

   Inserts

   Cas9
   SheBle
   

   Use

   CRISPR; Diatom, phaeodactylum

   Tags

   Phaeodactylum fcp promoter and terminator and YFP

   Expression

   Mutation

   Phaeodactylum Fcp promoter

   Promoter

   Phaeodactylum Fcp

   Available since

   July 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. AsCas12a-DR-cr in PBCSK-

   Plasmid

   #126640

   Purpose

   Cloning vector AsCas12a DR-crRNA for expression in mammalian cells

   Depositor

   Scot Wolfe

   Article

   Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

   Insert

   AsCas12a Full Length Direct Repeat crRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. PB-CMV-TO-Casp3te

   Plasmid

   #116055

   Purpose

   Reporter plasmid for measuring protease activity

   Depositor

   Michael Elowitz

   Article

   Gao et al Science. 2018 Sep 21;361(6408):1252-1258

   Insert

   Casp3te
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. SaCas9 TadCBEa

   Plasmid

   #193844

   Purpose

   Express TadCBEa (with SaCas9) in mammalian cells

   Depositor

   David Liu

   Article

   Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

   Insert

   SaCas9 TadCBEa
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX165-HypaCas9

   Plasmid

   #140567

   Purpose

   Expresses HypaCas9 in mammalian cells

   Depositor

   Feng Zhang

   Article

   Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

   Insert

   Cas9
   

   Use

   Tags

   Expression

   Mutation

   N692A M694A Q695A H698A

   Promoter

   Available since

   May 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMonAID_dCas9-PmCDA_Hyg_ALS

   Plasmid

   #91692

   Purpose

   Monocot Target-AID vector expressing rice-optimized dCas9-PmCDA1 with sgRNA targeting OsAls

   Depositor

   Akihiko Kondo

   Article

   Shimatani et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

   Insert

   SpCas9
   

   Use

   Tags

   PmCDA1

   Expression

   Plant

   Mutation

   D10A and H840A for dead Cas9

   Promoter

   2x35S

   Available since

   June 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMA-SpCas9-g9

    Plasmid

    #80792

    Purpose

    BsaI-based cloning of SpCas9 gRNA guide sequence, position 9/19/29 in the array

    Depositor

    Yonglun Luo

    Article

    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

    Insert

    CRISPR gRNA expression cassette (for SpCas9)
    

    Use

    CRISPR

    Tags

    na

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMA-SpCas9-g8

    Plasmid

    #80791

    Purpose

    BsaI-based cloning of SpCas9 gRNA guide sequence, position 8/18/28 in the array

    Depositor

    Yonglun Luo

    Article

    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

    Insert

    CRISPR gRNA expression cassette (for SpCas9)
    

    Use

    CRISPR

    Tags

    na

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. CL7_2xNLS_iGeoCas9(G)_2xNLS_His

    Plasmid

    #222989

    Purpose

    Bacterial expression of iGeoCas9(G) construct

    Depositor

    Jennifer Doudna

    Article

    Chen et al bioRxiv [Preprint]. 2023 Nov 15:2023.11.

    Insert

    CL7_2xNLS_iGeoCas9(G)_2xNLS_His
    

    Use

    CRISPR

    Tags

    CL7 and His

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTetQCas-8+IS186

    Plasmid

    #170636

    Purpose

    Expression of CRISPR-associated transposases

    Depositor

    Sheng Yang

    Article

    Zhang et al CRISPR J. 2021 Jun;4(3):350-359. doi: 10

    Insert

    VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(8+IS186 array)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCJH059_proD_Cas12c_DualRNA-GFP_CAM

    Plasmid

    #183076

    Purpose

    Plasmid expressing Cas12c tracrRNA and a GFP-targeting pre-crRNA separately.

    Depositor

    Jennifer Doudna

    Article

    Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

    Insert

    Cas12c tracrRNA and pre-crRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMCSG7-dNmeCas9

    Plasmid

    #71475

    Purpose

    bacterial pMCSG7 expression vector expressing catalytically inactive Nme cas9 with T7 promoter, N-terminal His tag and TEV site

    Depositor

    Erik Sontheimer

    Article

    Zhang et al Mol Cell. 2015 Oct 15;60(2):242-55. doi:

    Insert

    pMCSG7-Inactive-NmeCas9
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    D16A H588A

    Promoter

    Available since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBZCas13b-HEPN

    Plasmid

    #89901

    Purpose

    Bacterial expression for bzCas13b and crRNA with both HEPN domains mutated. New spacers can be cloned by digesting with BsaI.

    Depositor

    Feng Zhang

    Article

    Smargon et al Mol Cell. 2017 Feb 16;65(4):618-630.e7.

    Insert

    Cas13b
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    R116A/H121A/R1177A/H1182A

    Promoter

    Lac

    Available since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYPQ284 (Mb2Cas12a)

    Plasmid

    #138116

    Purpose

    Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized Mb2Cas12a without promoter

    Depositor

    Yiping Qi

    Article

    Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

    Insert

    Mb2Cas12a
    

    Use

    CRISPR; Gateway compatible cas12a entry clone

    Tags

    NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCLV3Cas-LT0

    Plasmid

    #210548

    Purpose

    Binary vector expressing the Lineage Tracing system. Cas9 expression driven by CLV3 promoter. LT0 reporter

    Depositor

    Ortrun Mittelsten Scheid

    Article

    Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

    Insert

    CLV3p::spCas9::PEA3At::U6p::AAVS1gRNA::pHTR5::LT0::mCherrySSM
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGGB-SpCas9

    Plasmid

    #210533

    Purpose

    Cloning A.thaliana codon optimized SpCas9 CDS as GreenGate module B

    Depositor

    Ortrun Mittelsten Scheid

    Article

    Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

    Insert

    SpCas9
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTetQCas-BsaI

    Plasmid

    #170629

    Purpose

    Expression of CRISPR-associated transposases

    Depositor

    Sheng Yang

    Article

    Zhang et al CRISPR J. 2021 Jun;4(3):350-359. doi: 10

    Insert

    VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(BsaI)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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