We narrowed to 59,942 results for: %s

1. lentiCRISPR.sgKras.9

   Plasmid

   #91894

   Purpose

   sgRNAs targeting mouse Kras. 3rd generation lentiviral backbone.

   Depositor

   Wen Xue

   Article

   Mou et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

   Insert

   Kras sgRNA
   

   Use

   Lentiviral

   Promoter

   U6

   Available Since

   Dec. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-HA-TFAP4(E135A/S139A)

   Plasmid

   #110157

   Purpose

   Expression of human TFAP4 (E135A/S139A) in mammalian cells

   Depositor

   Daniele Guardavaccaro

   Article

   D'Annibale et al J Biol Chem. 2014 Mar 14;289(11):7730-7.

   Insert

   TFAP4 (transcription factor AP-4) (TFAP4 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   E135A/S139A

   Promoter

   CMV

   Available Since

   May 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. dmBACCS2NS-IRES-dOrai-IRES-mCherry

   Plasmid

   #72896

   Purpose

   Mammalian expression of dmBACCS2NS, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and mCherry

   Depositor

   Takao Nakata

   Article

   Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

   Insert

   dmBACCS2NS-IRES-dOrai-IRES-mCherry
   

   Tags

   IRES-mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. 1068 pGL3 FasL promoter (no SV40 prom)

   Plasmid

   #9029

   Depositor

   William Sellers

   Article

   Nakamura et al Mol Cell Biol. 2000 Dec . 20(23):8969-82

   Insert

   Fas ligand promoter (FASLG Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Removed the SV40 promoter from pGL3

   Available Since

   April 4, 2006

   Availability

   Academic Institutions and Nonprofits only

5. attB‐mCh­‐Poly(A)-­PuroR

   Plasmid

   #65529

   Purpose

   PuroR containing plasmid for the generation of gene disruptions via Bxb1-mediated recombination into MIN-tagged cell lines. This vector can also be used to express cDNAs.

   Depositor

   Sebastian Bultmann

   Article

   Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

   Insert

   attB-mCherry
   

   Use

   Mouse Targeting; Bxb1

   Expression

   Mammalian

   Available Since

   Aug. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. ins:BB1.0L; cryaa:RFP

   Plasmid

   #110283

   Purpose

   A zebrafish insulin promoter driving the Brainbow (BB1.0L) cassette. Contains red eye marker, and is flanked with I-SceI sites.

   Depositor

   Nikolay Ninov

   Article

   Singh et al Nat Commun. 2017 Sep 22;8(1):664. doi: 1

   Insert

   Brainbow1.0L
   

   Use

   Zebrafish

   Promoter

   insulin

   Available Since

   May 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGL-p21UTRm1

   Plasmid

   #20878

   Depositor

   Robert Blelloch

   Article

   Wang et al Nat Genet. 2008 Dec . 40(12):1478-83.

   Insert

   p21 3'UTR site 1 mutation (Cdkn1a Mouse)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   Predicted miRNA binding site 1 (Position ~420) GC…

   Available Since

   Aug. 5, 2009

   Availability

   Academic Institutions and Nonprofits only

8. TIA1 RRM-CFP-FRBx5

   Plasmid

   #103780

   Purpose

   expresses iPOLYMER component in mammalian cells

   Depositor

   Takanari Inoue

   Article

   Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

   Insert

   TIA1 RRM-CFP-FRBx5
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET-21a_2xNLS_FnCpf1_MBP_protein_expression

   Plasmid

   #120801

   Purpose

   Protein expression plasmid for 2xNLS FnCpf1 in pET-21a backbone

   Depositor

   Scot Wolfe

   Article

   Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

   Insert

   2xNLS_FnCpf1
   

   Use

   CRISPR

   Tags

   MBP and NLS

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDONR223-SLC7A11 sh926R

    Plasmid

    #118701

    Purpose

    entry clone for SLC7A11 insensitive to hairpin TRCN0000288926

    Depositor

    William Kaelin

    Article

    Briggs et al Cell. 2016 Jun 30;166(1):126-39. doi: 10

    Insert

    SLC7A11 (SLC7A11 Human)
    

    Expression

    Mammalian

    Mutation

    wt , SLC7A11 sh926R

    Available Since

    Nov. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMSCV-puro-5A, 6A mMCL1-OM

    Plasmid

    #45818

    Depositor

    Joseph Opferman

    Article

    Perciavalle et al Nat Cell Biol. 2012 Apr 29;14(6):575-83.

    Insert

    5A, 6A mMCL-1-OM
    

    Expression

    Mammalian

    Mutation

    Changed Arginine to Alanine at 5th and 6th amino …

    Available Since

    July 24, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA3 PARL-FLAG-CT S65A+T69A+S70A

    Plasmid

    #13616

    Depositor

    Luca Pellegrini

    Article

    Jeyaraju et al Proc Natl Acad Sci U S A. 2006 Dec 5. 10

    Insert

    Presenilin-associated rhomboid-like (PARL, PARL Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed Ser 65, Thr 69 and Ser 70 to Alanine

    Available Since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA-FLAG-NEMO-C54/347A

    Plasmid

    #27268

    Depositor

    Thomas Gilmore

    Article

    Herscovitch et al Biochem Biophys Res Commun. 2008 Feb 29.

    Insert

    NEMO (IKBKG Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Cys54Ala Cys347Ala

    Available Since

    Feb. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA5FRT/TO-p97-RH-mycStrep

    Plasmid

    #31839

    Depositor

    Hemmo Meyer

    Article

    Ritz et al Nat Cell Biol. 2011 Aug 7;13(9):1116-23.

    Insert

    p97
    

    Tags

    Strep and myc

    Expression

    Bacterial and Mammalian

    Mutation

    R155H

    Promoter

    CMV

    Available Since

    Oct. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

15. pCMV-mGold-CAF1-C-10

    Plasmid

    #158003

    Purpose

    Visualization of nucleus in mammalian cells using mGold

    Depositor

    Francois St-Pierre

    Article

    Lee et al Sci Adv. 2020 Oct 23;6(43). pii: 6/43/ea

    Insert

    CAF1 (Chaf1a Mouse)
    

    Tags

    mGold

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLV.U6.gLacZ.Cas9-T2A-GFP

    Plasmid

    #170361

    Purpose

    Negative control plasmid used for Crispr/cas9 based disruption.

    Depositor

    Johan Jakobsson

    Article

    Jonsson et al Nat Commun. 2019 Jul 18;10(1):3182. doi:

    Insert

    Cas9-T2A-GFP
    

    Use

    Lentiviral

    Promoter

    U6

    Available Since

    June 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLV-EF1a-V5-Emerin

    Plasmid

    #120246

    Purpose

    Expresses V5-tagged Emerin in lentiviral vector

    Depositor

    Larry Gerace

    Article

    Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

    Insert

    Emerin (Emd Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Promoter

    EF1a

    Available Since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFUW-TetO-Txnip

    Plasmid

    #178437

    Purpose

    doxycycline-inducible expression of Mouse Txnip in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

    Insert

    Txnip (Txnip Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGL-p21UTRm2

    Plasmid

    #20879

    Depositor

    Robert Blelloch

    Article

    Wang et al Nat Genet. 2008 Dec . 40(12):1478-83.

    Insert

    p21 3'UTR site 2 mutation (Cdkn1a Mouse)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    Predicted miRNA binding site 2 (Position ~1100) G…

    Available Since

    March 12, 2012

    Availability

    Academic Institutions and Nonprofits only

20. pUPD2 PhiC31 attP:TMtb:P35S:attB (GB1494)

    Plasmid

    #160577

    Purpose

    35S promoter sequence and the Mtb terminator flanked by two opposing PhiC31 site-specific recombination sites (attP and attB)

    Depositor

    Diego Orzaez

    Article

    Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

    Insert

    PhiC31 PB (attP:TMtb:P35S:attB)
    

    Use

    Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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