We narrowed to 12,610 results for: NSI
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Plasmid#22277PurposeExpresses Tiam DH-PH domains fused to mYFP-PIF6, for optogenetic recruitmentDepositorInsertTiamDHPH-20aaLinker-mYFP-PIF6APB (PIL2 Mustard Weed)
UseSynthetic BiologyExpressionMammalianPromoterSV40Available SinceOct. 21, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-ChRger1-TS-EYFP
Plasmid#127243PurposeHigh photocurrent, low-light sensitive channelrhodopsin (ChRger1) for optogenetic activation with systemic delivery or for low-light activation. Driven by human Synapsin I promoter.DepositorInsertChRger1-TS-EYFP
UseAAVTagsEYFP and SpyTagExpressionMammalianPromoterhSyn1Available SinceSept. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJT9R
Plasmid#98305PurposeOverexpressing yeast farnesyl pyrophosphate synthase Erg20p and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of GAL1/GAL2 promoterDepositorInsertTRPL3AcNES1>TRPL41B
ExpressionYeastAvailable SinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET22b_EcSSB_2
Plasmid#78204PurposeExpression of single-cysteine variant of single-stranded DNA-binding protein protein, EcSSB, as scaffold for ssDNA biosensorDepositorInsertssb (W88C)
ExpressionBacterialMutationW88C (please see depositor comment below)PromoterT7Available SinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFR-mScarletI-antiFRET-P2A-G-Ca-FLITS
Plasmid#191457PurposeCompare the intensity of the genetically encoded calcium sensor (GECI) G-Ca-FLITS to mScarlet-I fluorescent proteinDepositorInsertG-Ca-FLITS
TagsHis-mScarletI-antiFRETlinker-P2AExpressionBacterial and MammalianAvailable SinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUBC_V5_MS2-mVenus
Plasmid#107606PurposeTransient expression of humanized MS2 (V75E/A81G), fused to mVenus in mammalian cells, under a hUBC promoter, V5 tagDepositorInsertMS2-mVenus
UseSynthetic BiologyTagsV5 and mVenusMutationMS2: V75E/A81GPromoterhuman UBCAvailable SinceMay 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
LRP6N-IgG-pCS2
Plasmid#27279DepositorInsertlow density lipoprotein receptor-related protein 6 (LRP6 Human)
UseXenopus injectionTagsIgG FcAvailable SinceFeb. 10, 2011AvailabilityAcademic Institutions and Nonprofits only -
P447-FBP_19
Plasmid#139710PurposeT-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway, Luz promoter is ABA responsiveDepositorInsertpSlH4:NnCPH:tSlH4, pSlRBCS2:AsNpga:tAtuG7, pAtuMas:NnH3H:tAtuMas, p35S:NnHisps:t35S, pAtRAB18:NnLuz:tAtuOcs
UseLuciferaseExpressionPlantPromotervariousAvailable SinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR SFFVp BFP-PIF-SOS2cat
Plasmid#50850PurposeOptogenetic Ras activation system; cytoplasmic componentDepositorAvailable SinceJan. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pXJ-Myc-FOXA1
Plasmid#232645Purposetransient expresses FOXA1 in mammalian cellsDepositorAvailable SinceMarch 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
LRP6-m5-pCS2-VSVG
Plasmid#27283DepositorInsertlow density lipoprotein receptor-related protein 6 (LRP6 Human)
UseXenopus injectionTagsVSVGMutationChanged S/T of all five PPP(S/T)P motifs to Alani…Available SinceMarch 3, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET28a-B4E
Plasmid#162067PurposeFor expresion of the B4E fusion protein, consists of the ampR gene (encoding the enzyme β-lactamase without the native signal sequence) fused to residues 28-215 of the murine eIF4EDepositorInsertampR-eIF4E fusion protein
Tags6xHis tagExpressionBacterialMutationeIF4E incorporating the K119A mutation, which is …PromoterT7Available SinceFeb. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
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c3GIC9n
Plasmid#62192Purposecol1a1 targeting vector for inducible [TRE3G]-GFP-IRES-Cas9n (D10A variant) expression. Contains NsiI cloning site for U6-sgRNA cassettesDepositorInserthumanized S. Pyogenes Cas9 (D10A)
UseCRISPRTagsHAMutationD10A substitution (Cas9n)PromoterTRE3GAvailable SinceAug. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pVenus-N-SLC35B2
Plasmid#155038PurposeTransient expression of SLC35B2-VenusDepositorInsertSLC35B2 (SLC35B2 Human)
TagsVenusExpressionMammalianMutationSee depositor comments belowPromoterCMVAvailable SinceOct. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTorPE-G-GECO1.2
Plasmid#32468DepositorInsertG-GECO1.2
UseLab constructedTags6 His tag and Modified TorA MNNNDLFQASRRRFLAQLG[G…ExpressionBacterialMutationGCaMP3 K69E/N77Y/D86G/K119I/L173Q/D260G/S404G/E43…Available SinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(-)-EGFP-AP4-mito
Plasmid#229693PurposeTransient expression of EGFP AP4-mito in mammalian cellsDepositorInsertEGFP; Control construct with tandem AP4 motifs (mitochondria)
TagsEGFPExpressionMammalianPromoterCMV promoterAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFB-GTE-COI1
Plasmid#29516DepositorInsertCOI1 (COI1 Mustard Weed)
TagsGST and TEV cleavage siteExpressionInsectMutationNote that the first AA in TEV site is R, not EAvailable SinceMay 26, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGL3-PTEN 3'UTR
Plasmid#28104DepositorInsertPTEN 3'UTR (Pten Mouse)
ExpressionMammalianAvailable SinceApril 15, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAcBac1-haloTyrRS C6
Plasmid#141174PurposeExpression of orthogonal tRNA and tRNA synthetase for incorporation of unnatural amino acidsDepositorInsertC6 HaloTyr tRNA synthetase
UseLentiviralExpressionMammalianMutationL270S, Y271L, N311G, C313TPromoterCMVAvailable SinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only