We narrowed to 15,991 results for: LEA

1. SUMO_TwinStrep_Csx30

   Plasmid

   #197611

   Purpose

   For protein purification of Csx30

   Depositor

   Feng Zhang

   Article

   Strecker et al Science. 2022 Nov 25;378(6622):874-881.

   Insert

   SUMO_TwinStrep_Csx30
   

   Expression

   Bacterial

   Available Since

   May 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCDNA3_HA_Csx30

   Plasmid

   #197610

   Purpose

   Expresses a HA-tagged version of Csx30

   Depositor

   Feng Zhang

   Article

   Strecker et al Science. 2022 Nov 25;378(6622):874-881.

   Insert

   HA-tagged Csx30
   

   Expression

   Mammalian

   Available Since

   June 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Reb_81 aka PAS622

   Plasmid

   #71227

   Purpose

   Reb_1 with RebB(T28A, D72G)

   Depositor

   Pamela Silver

   Article

   Polka et al ACS Synth Biol. 2016 Feb 3.

   Insert

   Reb locus
   

   Tags

   none

   Expression

   Bacterial

   Mutation

   RebB(T28A, D72G), RebA(G53A)

   Available Since

   April 7, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pPS2035

   Plasmid

   #1368

   Depositor

   Pamela Silver

   Article

   Brodsky et al RNA 2000 Dec;6(12):1737-49.

   Insert

   U1A (1-94) (RNU1-4 Human)
   

   Tags

   GFP

   Expression

   Yeast

   Mutation

   U1A (1-94)

   Available Since

   Dec. 7, 2005

   Availability

   Academic Institutions and Nonprofits only

5. pBabe(SV40)-Cytochrome C-GFP

   Plasmid

   #41184

   Depositor

   Douglas Green

   Article

   Goldstein et al Nat Cell Biol. 2000 Mar;2(3):156-62.

   Insert

   Cytochrome C-GFP
   

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   SV40

   Available Since

   Nov. 26, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pCNCre3

   Plasmid

   #46088

   Depositor

   Raymond Monnat

   Article

   Monnat RJ et al Biochem Biophys Res Commun. 1999 Feb 5;2

   Insert

   CreI
   

   Tags

   NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pPS2042

   Plasmid

   #1373

   Depositor

   Pamela Silver

   Article

   Brodsky et al RNA 2000 Dec;6(12):1737-49.

   Insert

   U1A (1-94) (RNU1-4 Human)
   

   Tags

   GFP

   Expression

   Yeast

   Mutation

   U1A (1-94)

   Available Since

   Aug. 3, 2005

   Availability

   Academic Institutions and Nonprofits only

8. Reb_207 aka PAS630

   Plasmid

   #71235

   Purpose

   Reb_206 with RebA(S88P)

   Depositor

   Pamela Silver

   Article

   Polka et al ACS Synth Biol. 2016 Feb 3.

   Insert

   Reb locus
   

   Tags

   mNeonGreen to second copy RebB, soluble mCherry a…

   Expression

   Bacterial

   Mutation

   RebA(S88P)

   Available Since

   July 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCMV-UnaG-pA (pE091)

   Plasmid

   #149354

   Purpose

   Mammalian Expression vector for UnaG

   Depositor

   Ralf Kuehn

   Article

   Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

   Insert

   UnaG
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Jan. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGFP-HBx NESM

    Plasmid

    #24932

    Depositor

    Xin Wang

    Article

    Forgues et al J Biol Chem. 2001 Jun 22. 276(25):22797-

    Insert

    HBx
    

    Tags

    EGFP and HA

    Expression

    Mammalian

    Mutation

    L98A,G99A,L100A

    Available Since

    June 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Reb_82 aka PAS623

    Plasmid

    #71228

    Purpose

    Reb_1 with RebB(S82P, D88G)

    Depositor

    Pamela Silver

    Article

    Polka et al ACS Synth Biol. 2016 Feb 3.

    Insert

    Reb locus
    

    Tags

    none

    Expression

    Bacterial

    Mutation

    RebB(S82P, D88G), RebA (G53A)

    Available Since

    April 7, 2016

    Availability

    Academic Institutions and Nonprofits only

12. pNIC-HIS6-GST-TEV-AVITAG-GG

    Plasmid

    #215809

    Purpose

    Cloning of target gene with N terminal His6 GST tag and TEV cleavage non cleavable N-terminal avitag

    Depositor

    Frank von Delft, ASAP Discovery

    Article

    Fairhead et al bioRxiv 2024.02.13.579886

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    April 25, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pCS2TAL3-DDD

    Plasmid

    #48637

    Purpose

    Expression of TALEN proteins with FokI-DDD domain

    Depositor

    David Grunwald

    Article

    Hu et al PLoS One. 2013 May 7;8(5):e63074. doi: 1

    Type

    Empty backbone

    Use

    TALEN

    Tags

    Flag

    Promoter

    CMV

    Available Since

    Oct. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCS2TAL3-RRR

    Plasmid

    #48636

    Purpose

    Expression of TALEN proteins with FokI-RRR domain

    Depositor

    David Grunwald

    Article

    Hu et al PLoS One. 2013 May 7;8(5):e63074. doi: 1

    Type

    Empty backbone

    Use

    TALEN

    Tags

    HA-1 and HA-2

    Promoter

    CMV

    Available Since

    Oct. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEM7074

    Plasmid

    #214012

    Purpose

    PrpsM driven expression of ssrA-tagged mCerulean (ASV)

    Depositor

    Marc Erhardt

    Article

    Delgadillo-Guevara et al J Biol Eng. 2024 Apr 8;18(1):25. doi: 10

    Insert

    mCerulean-ASV
    

    Expression

    Bacterial

    Promoter

    PrpsM

    Available Since

    March 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. 2xmNeonGreen-hMeikin

    Plasmid

    #174704

    Purpose

    Stable expression of human Meikin

    Depositor

    Iain Cheeseman

    Article

    Maier et al Dev Cell. 2021 Aug 9;56(15):2192-2206.e8

    Insert

    Meikin
    

    Use

    Retroviral

    Tags

    2xmNeonGreen

    Expression

    Mammalian

    Available Since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. p2CT-Cdi

    Plasmid

    #83044

    Purpose

    Expresses Corynebacterium diphtheriae Cas9 (CdiCas9)

    Depositor

    Jennifer Doudna

    Article

    Ma et al Mol Cell. 2015 Nov 5;60(3):398-407. doi:

    Insert

    CdiCas9
    

    Tags

    His-MBP

    Expression

    Bacterial

    Available Since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

18. DinoIII-arrO

    Plasmid

    #128142

    Purpose

    Dinoflagellate backbone for rifampin resistant codon optimized gene

    Depositor

    Senjie Lin

    Article

    Sprecher et al Microorganisms. 2020 Jan 16;8(1). pii: m

    Insert

    Rifampin ADP-ribosylating transferase
    

    Expression

    Bacterial

    Promoter

    Dinolagellate potential promoters

    Available Since

    April 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pXcX-PRS-EGFP

    Plasmid

    #25865

    Depositor

    Sergey Kasparov

    Article

    Teschemacher et al Hypertension. 2008 Aug . 52(2):351-8.

    Insert

    PRS driven enhanced green fluorescent protein
    

    Expression

    Mammalian

    Available Since

    Aug. 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pISH-cre

    Plasmid

    #15933

    Depositor

    Peter Mombaerts

    Article

    Li et al Nature. 2004 Mar 25. 428(6981):393-9.

    Insert

    cre recombinase (cre )
    

    Mutation

    642C to T, 966A to G

    Available Since

    Jan. 24, 2012

    Availability

    Academic Institutions and Nonprofits only

21. pET21b-RL018A

    Plasmid

    #42137

    Depositor

    Sheref Mansy

    Article

    Lentini et al ACS Synth Biol. 2013 Mar 15.

    Insert

    mCerulean-mCherry
    

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    June 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. P100 (3OMC)

    Plasmid

    #28159

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    SND1 (SND1 Human)
    

    Tags

    His tag with TEV cleavage

    Expression

    Bacterial

    Mutation

    Tudor domain (residues 650-910).

    Available Since

    Feb. 28, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

23. No-repeats

    Plasmid

    #122487

    Purpose

    A control plasmid that is similar to PRExpress but lacks poly-PRE sequence. (GenBank: MF058599.1)

    Depositor

    Renato Paro

    Article

    Akmammedov et al Sci Rep. 2017 Jul 31;7(1):6899. doi: 10.

    Insert

    LacZ
    

    Expression

    Insect

    Available Since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pPS2045

    Plasmid

    #1376

    Depositor

    Pamela Silver

    Article

    Brodsky et al RNA 2000 Dec;6(12):1737-49.

    Insert

    U1A (1-94) (RNU1-4 Human)
    

    Tags

    GFP

    Expression

    Yeast

    Mutation

    U1A (1-94)

    Available Since

    Aug. 3, 2005

    Availability

    Academic Institutions and Nonprofits only

25. LIP-WT

    Plasmid

    #16657

    Depositor

    Bert Vogelstein

    Article

    Cheong et al Science. 2006 Nov 24. 314(5803):1308-11.

    Insert

    Liposomase
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    March 10, 2008

    Availability

    Academic Institutions and Nonprofits only

26. pISH-OCAM

    Plasmid

    #15932

    Depositor

    Peter Mombaerts

    Article

    Li et al Nature. 2004 Mar 25. 428(6981):393-9.

    Insert

    OCAM (Ncam2 Mouse)
    

    Available Since

    Jan. 24, 2012

    Availability

    Academic Institutions and Nonprofits only

27. pSCFRSF131

    Plasmid

    #118402

    Purpose

    for structure determination

    Depositor

    Hideo Iwai

    Article

    Iwai et al J Mol Biol. 2017 Dec 8;429(24):3942-3956

    Insert

    MjaTFIIB mini intein
    

    Expression

    Bacterial

    Available Since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pYPQ-Un1Cas12f1

    Plasmid

    #205143

    Purpose

    Un1Cas12f1 Gateway entry plasmid

    Depositor

    Yiping Qi

    Article

    Tang et al Plant Genome. 2024 May 28:e20465. doi: 1

    Insert

    Un1Cas12f1
    

    Use

    CRISPR

    Tags

    NLS

    Available Since

    June 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pNIC-HIS6-SUMO1-AVITAG-GG

    Plasmid

    #215808

    Purpose

    Cloning of target gene with N terminal His6 SUMO2 tag and SENP1 cleavage non cleavable N-terminal avitag

    Depositor

    Frank von Delft, ASAP Discovery

    Article

    Fairhead et al bioRxiv 2024.02.13.579886

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    April 25, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

30. pEM7065

    Plasmid

    #214003

    Purpose

    PrpsM driven expression of ssrA-tagged mCerulean (LVA)

    Depositor

    Marc Erhardt

    Article

    Delgadillo-Guevara et al J Biol Eng. 2024 Apr 8;18(1):25. doi: 10

    Insert

    mCerulean-LVA
    

    Expression

    Bacterial

    Promoter

    PrpsM

    Available Since

    March 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pACS77

    Plasmid

    #204585

    Purpose

    GST-lamin A (389-638)

    Depositor

    Tom Misteli

    Article

    Schibler et al Elife. 2023 May 23;12:e80653. doi: 10.75

    Insert

    lamin A (389-638)
    

    Tags

    GST-lamin A (389-638)

    Expression

    Bacterial

    Available Since

    Jan. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pACS69

    Plasmid

    #204584

    Purpose

    GST-lamin A (389-566)

    Depositor

    Tom Misteli

    Article

    Schibler et al Elife. 2023 May 23;12:e80653. doi: 10.75

    Insert

    lamin A (389-566)
    

    Tags

    GST-lamin A (389-566)

    Expression

    Bacterial

    Available Since

    Jan. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pcDNA3-C17orf53_1-241-mCer

    Plasmid

    #211443

    Purpose

    mammalian expression of the N-terminal amino acids 1-241 of human C17orf53 C-terminally fused to mCerulean

    Depositor

    Helmut Pospiech

    Insert

    C17orf53 (HROB Human)
    

    Tags

    mCerulean

    Expression

    Mammalian

    Mutation

    Deletion of aa 242-646

    Available Since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pACS124

    Plasmid

    #204592

    Purpose

    GST-lamin A (551-646)

    Depositor

    Tom Misteli

    Article

    Schibler et al Elife. 2023 May 23;12:e80653. doi: 10.75

    Insert

    human lamin A (551-646)
    

    Tags

    GST - lamin A (551-646)

    Expression

    Bacterial

    Available Since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pACS122

    Plasmid

    #204591

    Purpose

    GST-lamin A (451-646)

    Depositor

    Tom Misteli

    Article

    Schibler et al Elife. 2023 May 23;12:e80653. doi: 10.75

    Insert

    human lamin A (451-646)
    

    Tags

    GST - lamin A (451-646)

    Expression

    Bacterial

    Available Since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pACS37

    Plasmid

    #204589

    Purpose

    GST-lamin A (506-646)

    Depositor

    Tom Misteli

    Article

    Schibler et al Elife. 2023 May 23;12:e80653. doi: 10.75

    Insert

    human lamin A (506-646)
    

    Tags

    GST - lamin A (506-646)

    Expression

    Bacterial

    Available Since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pACS71

    Plasmid

    #204588

    Purpose

    GST-lamin A (389-597)

    Depositor

    Tom Misteli

    Article

    Schibler et al Elife. 2023 May 23;12:e80653. doi: 10.75

    Insert

    human lamin A (389-597)
    

    Tags

    GST-lamin A (389-597)

    Expression

    Bacterial

    Available Since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pACS73

    Plasmid

    #204587

    Purpose

    GST-lamin A (389-609)

    Depositor

    Tom Misteli

    Article

    Schibler et al Elife. 2023 May 23;12:e80653. doi: 10.75

    Insert

    human lamin A (389-609)
    

    Tags

    GST-lamin A (389-609)

    Expression

    Bacterial

    Available Since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pACS75

    Plasmid

    #204586

    Purpose

    GST-lamin A (389-626)

    Depositor

    Tom Misteli

    Article

    Schibler et al Elife. 2023 May 23;12:e80653. doi: 10.75

    Insert

    lamin A (389-626)
    

    Tags

    GST-lamin A (389-626)

    Expression

    Bacterial

    Available Since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pACS58

    Plasmid

    #204583

    Purpose

    GST-lamin A (565-646)

    Depositor

    Tom Misteli

    Article

    Schibler et al Elife. 2023 May 23;12:e80653. doi: 10.75

    Insert

    lamin A (565-646)
    

    Tags

    GST-lamin A (565-646)

    Expression

    Bacterial

    Available Since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pACS57

    Plasmid

    #204582

    Purpose

    GST-lamin C (389-572)

    Depositor

    Tom Misteli

    Article

    Schibler et al Elife. 2023 May 23;12:e80653. doi: 10.75

    Insert

    lamin C (389-572)
    

    Tags

    GST-lamin C (389-572)

    Expression

    Bacterial

    Available Since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. tagRFP-IMBB4-pUC

    Plasmid

    #170769

    Purpose

    test plasmid for 5RM assembly, encodes fluorescent red protein

    Depositor

    Ichiro Matsumura

    Article

    Matsumura et al Biotechniques. 2022 May;72(5):185-193. d

    Insert

    tagRFP
    

    Promoter

    none

    Available Since

    Feb. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. Venus-79aa-ActA-pEGFP-C1

    Plasmid

    #166763

    Purpose

    To target Venus to the mitochondrial outer membrane. ActA tail anchor inserts into the outer membrane and Venus faces the cytosol. Serves as a collisional control in FLIM-FRET experiments.

    Depositor

    David Andrews

    Insert

    ActA tail anchor (Listeria monocytogenes) (actA Listeria monocytogenes, Synthetic)
    

    Tags

    Venus

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pmCell-Free_KA1_SARS-COV-2_S-frag3

    Plasmid

    #169403

    Purpose

    Cell-free/mammalian expression vector for SARS-COV-2 virus N-term fragment of S protein after the furin cleavag with N-term eGFP

    Depositor

    Kirill Alexandrov

    Insert

    N-term frag. S protein after the furin cleavag (N SARS-Cov2)
    

    Use

    Cell-free expression vector

    Tags

    His and eGFP

    Promoter

    T7- CMV/CAG

    Available Since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pmCellFree_KA1_SARS-COV-2_S-frag5

    Plasmid

    #169404

    Purpose

    Cell-free/mammalian expression vector for SARS-COV-2 virus C-terminal ectodomain of S protein from the furin cleavag with N-term eGFP

    Depositor

    Kirill Alexandrov

    Insert

    C-term frag. S protein after the furin cleavag (S SARS-Cov2)
    

    Use

    Cell-free expression vector

    Tags

    His and eGFP

    Promoter

    T7- CMV/CAG

    Available Since

    June 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pBHRSF63

    Plasmid

    #118403

    Purpose

    for structure determination

    Depositor

    Hideo Iwai

    Article

    Iwai et al J Mol Biol. 2017 Dec 8;429(24):3942-3956

    Insert

    MvuTFIIB mini intein
    

    Expression

    Bacterial

    Available Since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pBHDuet33

    Plasmid

    #118397

    Purpose

    analysis of cis-splicing

    Depositor

    Hideo Iwai

    Article

    Iwai et al J Mol Biol. 2017 Dec 8;429(24):3942-3956

    Insert

    MvuTFIIB intein
    

    Expression

    Bacterial

    Available Since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pET15b-PvuRts1I

    Plasmid

    #86887

    Purpose

    express N-term His tagged PvuRts1I in ER2566 strain

    Depositor

    Matthias Bochtler

    Article

    Kazrani et al Nucleic Acids Res. 2014 May;42(9):5929-3

    Insert

    PvuRts1I (pvuRts1I )
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Feb. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pDL96

    Plasmid

    #43910

    Depositor

    Stephen Elledge

    Article

    Lee et al Mol Cell. 2008 Oct 10;32(1):70-80. doi:

    Insert

    DIF1 (DIF1 Budding Yeast)
    

    Expression

    Yeast

    Mutation

    AAEAAA

    Available Since

    Aug. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

50. VTA

    Plasmid

    #27805

    Depositor

    Steven Vogel

    Article

    Koushik et al J Biomed Opt. 2008 May-Jun;13(3):031204.

    Insert

    Venus Traf Amber
    

    Expression

    Mammalian

    Available Since

    July 5, 2011

    Availability

    Academic Institutions and Nonprofits only