We narrowed to 7,357 results for: ef1a

1. LV_EF1a_MEK1(S218D,S222D)-P2A-Hygro_Barcode

   Plasmid

   #170240

   Purpose

   Barcoded lentiviral vector to express MEK1 (S218D, S222D) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

   Depositor

   Prashant Mali

   Article

   Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

   Insert

   MAP2K1 (MAP2K1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   S218D, S222D

   Promoter

   EF1a

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. Lv EF1a HP1cs-Frb1x PGK Puro

   Plasmid

   #102808

   Purpose

   Lentiviral plasmid from FIRE-Cas9 system to express HP1cs-Frb1x fusion protein

   Depositor

   Jerry Crabtree

   Article

   Braun et al Nat Commun. 2017 Sep 15;8(1):560. doi: 1

   Insert

   HP1cs-Frb1x
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-Ef1a-DO-NpHR3.0-eYFP-WPRE-pA

   Plasmid

   #37087

   Purpose

   Expresses NpHR3.0-eYFP in cells lacking Cre (Cre-Off), for optogenetic inhibition

   Depositor

   Bernardo Sabatini

   Article

   Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

   Insert

   NpHR3.0-EYFP
   

   Use

   AAV and Cre/Lox; Cre-off

   Tags

   EYFP

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   July 27, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. P-Ef1a-mCherry-T2A-RPL22-1xV5

   Plasmid

   #170324

   Purpose

   Expresses V5 and mCherry tagged RPL22

   Depositor

   Hemali Phatnani

   Article

   Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

   Insert

   RPL22 (RPL22 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   None

   Promoter

   Available since

   May 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. LV_EF1a_FLAG-Mkk7-JNK2-P2A-Hygro_Barcode

   Plasmid

   #170228

   Purpose

   Barcoded lentiviral vector to express Mkk7-JNK2 fusion in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

   Depositor

   Prashant Mali

   Article

   Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

   Insert

   Map2k7-MAPK9 fusion (MAPK9, Map2k7 Mouse, Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   Fusion

   Promoter

   EF1a

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHAGE2-EF1a-MCS-hPOT1_IRES-blast

   Plasmid

   #166411

   Purpose

   express hPOT1 in mammalian cells

   Depositor

   Agnel Sfeir

   Article

   Pinzaru et al Genes Dev. 2020 Dec 1;34(23-24):1619-163

   Insert

   hPOT1 (POT1 Human)
   

   Use

   Lentiviral

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Promoter

   ef-1a

   Available since

   May 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV-EF1a-MinE-IRES-Blast

   Plasmid

   #213115

   Purpose

   Expression of untagged MinE in mammalian cells

   Depositor

   Scott Coyle

   Article

   Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

   Insert

   MinE (minE )
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOTTC446 - pAAV EF1a hPREP(S554A)

   Plasmid

   #59968

   Purpose

   An AAV packaging vector that expresses catalytically-inactive hPREP under control of the EF1a promoter.

   Depositor

   Brandon Harvey

   Article

   Savolainen et al Neurobiol Dis. 2014 Aug;68:1-15. doi: 10

   Insert

   Prolyl oligopeptidase (PREP Human)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   S554A

   Promoter

   EF1a

   Available since

   Oct. 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-EF1a-SPdCas9-DNMT3B-2A-Blast

   Plasmid

   #71217

   Purpose

   dCas9 fused to human DNMT3B catalytic domain

   Depositor

   Yonglun Luo

   Article

   Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

   Insert

   dSpCas9-DNMT3B catalytic domain
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-EF1a-FRT-JEDI-1P-Kv-WPRE

    Plasmid

    #202617

    Purpose

    Genetically encoded voltage indicator (GEVI) JEDI-1P flanked by FRT in AAV production vector expressed under the mammalian promoter (EF1a), FLP recombinase-dependent

    Depositor

    Francois St-Pierre

    Article

    Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

    Insert

    FRT-JEDI-1P-Kv
    

    Use

    AAV; Flp/frt

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-Ef1a-DIO-VGAT-WPRE-pA

    Plasmid

    #39320

    Depositor

    Bernardo Sabatini

    Article

    Tritsch et al Nature. 2012 Oct 11;490(7419):262-6. doi

    Insert

    Slc32a1 (Slc32a1 Mouse)
    

    Use

    AAV; R

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 9, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAF0286 Ef1a-Cp36_attB-BFP acceptor

    Plasmid

    #193474

    Purpose

    Acceptor plasmid for 3 plasmid recombination assay

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Cp36_attB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKLV2-U6(BsmBI)-EF1a-BFP-BSD

    Plasmid

    #117136

    Purpose

    Empty vector encoding BFP-BSD

    Depositor

    Kosuke Yusa

    Insert

    BSD2A
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    modified BFP

    Promoter

    human U6 promoter, human EF1a promoter

    Available since

    April 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-EF1a-DIO-GRAB_r5-HT1.0

    Plasmid

    #208725

    Purpose

    Expresses the red 5-HT sensor GRAB_r5-HT1.0 in neurons in the presence of Cre recombinase

    Depositor

    Yulong Li

    Article

    Deng et al bioRxiv 2023.05.27.542566

    Insert

    Red fluorescent 5-HT sensor GRAB_r5-HT1.0
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. PB-Ef1a-SunTag-RPS7-PP7-WPRE

    Plasmid

    #198338

    Purpose

    PB plasmid expressing RPS7 mRNA tagged with SunTag repeats and PP7 stem loops

    Depositor

    Michael Ward

    Article

    De Pace et al Nat Neurosci. 2024 Apr 10. doi: 10.1038/

    Insert

    GCN4x24-RPS7-3'UTR-PP7 stem loops x24
    

    Use

    Piggybac

    Tags

    PP7 stem loops and SunTag

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGL3-U6-XsgRNA-ccdB-EF1a-Puromycin

    Plasmid

    #115519

    Purpose

    To construct multiple sgRNA expression vector for CRISPR

    Depositor

    Xingxu Huang

    Insert

    ccdB-sgRNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. ssAAV-EF1a-FLuc-WPRE-HgHpA_bac_293

    Plasmid

    #118412

    Purpose

    Expresses firefly luciferase under the control of a strong ubiquitous promoter EF1a along with a WPRE cassette. Backbone is compatible with both baculoviral and human production methods of AAV.

    Depositor

    Nicole Paulk

    Article

    Rumachik et al Mol Ther Methods Clin Dev. 2020 May 22;1

    Insert

    Firefly luciferase
    

    Use

    AAV, Luciferase, and Synthetic Biology

    Tags

    Expression

    Insect and Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDH-EF1a-hLMX1A-T2A-copGFP

    Plasmid

    #170444

    Purpose

    Lentiviral vector expressing human LMX1A (with silent mutation) with copGFP reporter gene, under control of EF1alpha promoter.

    Depositor

    Sang-Hun Lee

    Article

    Wulansari et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabb

    Insert

    human LMX1A
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-Ef1a-Coff/Fon-oScarlet

    Plasmid

    #137138

    Purpose

    Intersectional viral expression of oScarlet in cells expressing Flp AND NOT Cre

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Coff/Fon-oScarlet
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    E95D

    Promoter

    EF1a

    Available since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-EF1a-DIO-GRAB_g5-HT3.0mut

    Plasmid

    #208724

    Purpose

    Expresses the green 5-HT sensor GRAB_g5-HT3.0mut in neurons in the presence of Cre recombinase

    Depositor

    Yulong Li

    Article

    Deng et al bioRxiv 2023.05.27.542566

    Insert

    Green fluorescent 5-HT sensor GRAB_g5-HT3.0mut
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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