We narrowed to 7,283 results for: ef1a;

1. pCDH-CAG-IntCCCre-EF1a-Cerulean

   Plasmid

   #160508

   Purpose

   Expresses IntCCCre in mammalian cells in split intein-mediated split-Cre recombinase applications

   Depositor

   Shawn Je

   Article

   Khoo et al Mol Brain. 2020 Jul 2;13(1):101. doi: 10

   Insert

   IntCCCre
   

   Use

   Cre/Lox and Lentiviral

   Tags

   mCerulean

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Jan. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSicoR-Ef1a-mCh-Puro-Luci

   Plasmid

   #31850

   Depositor

   Bruce Conklin

   Article

   Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

   Insert

   Luciferase RNAi
   

   Use

   Cre/Lox, Lentiviral, and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 for shRNA; Ef1alpha for mCherry-puromycin

   Available since

   Jan. 3, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRRLNeo-pEF1a-p53ash-mKate2-splitmVenusN

   Plasmid

   #69582

   Purpose

   Expresses p53 tagged with mKate2 and split N-term mVenus

   Depositor

   Galit Lahav

   Article

   Gaglia et al Mol Syst Biol. 2014 Oct 24;10:753. doi:

   Insert

   p53 (TP53 Human)
   

   Use

   Lentiviral

   Tags

   mKate2 and split mVenus - N term

   Expression

   Mammalian

   Mutation

   7 silent mutations in p53 DNA to prevent targetin…

   Promoter

   EF1alpha

   Available since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEF1a-RoninDHSA-FLAG-IRES-Neo

   Plasmid

   #28021

   Depositor

   Thomas Zwaka

   Article

   Dejosez et al Genes Dev. 2010 Jul 15. 24(14):1479-84.

   Insert

   Ronin (THAP11 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   DHSA mutation (Y246A) of the previously defined H…

   Promoter

   Available since

   May 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDH-EF1a-tetR-T2A-tSapphire

   Plasmid

   #176312

   Purpose

   construct expresses tet repressor (tetR) to reduce leakiness together with the fluorochrome t-Sapphire

   Depositor

   Irmela Jeremias

   Article

   Liu et al Biomark Res. 2020 Sep 16;8:46. doi: 10.1

   Inserts

   tetR
   T-sapphire
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-EF1a-CCre-MBP1-WPRE

   Plasmid

   #193917

   Purpose

   Expresses one of the components of Cre-DOR_N6C1 (RFP-dependent Cre)

   Depositor

   Tatsushi Onaka

   Article

   Inutsuka et al Commun Biol. 2022 Sep 16;5(1):979. doi:

   Insert

   CCre-MBP1
   

   Use

   AAV, Affinity Reagent/ Antibody, Cre/Lox, and Syn…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-EF1a-NCre-MBP6-WPRE

   Plasmid

   #193916

   Purpose

   Expresses one of the components of Cre-DOR_N6C1 (RFP-dependent Cre)

   Depositor

   Tatsushi Onaka

   Article

   Inutsuka et al Commun Biol. 2022 Sep 16;5(1):979. doi:

   Insert

   NCre-MBP6
   

   Use

   AAV, Affinity Reagent/ Antibody, Cre/Lox, and Syn…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEF1a-myc-LRH1(ES)-IRES-neo

   Plasmid

   #28092

   Depositor

   Austin Cooney

   Article

   Wagner et al Stem Cells. 2010 Oct . 28(10):1794-804.

   Insert

   Lrh-1 (Nr5a2 Mouse)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 15, 2011

   Availability

   Academic Institutions and Nonprofits only

9. pLEX-EF1a ANXA11-mEmerald R346C

   Plasmid

   #164213

   Purpose

   pLEX lentivirus backbone expresses mEmerald tagged ANXA11 R346C mutant under EF1a promoter

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

   Insert

   ANXA11 (ANXA11 Human)
   

   Use

   Lentiviral

   Tags

   mEmerald

   Expression

   Mutation

   R346C

   Promoter

   EF1a

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLEX-EF1a ANXA11-mEmerald R235Q

    Plasmid

    #164212

    Purpose

    pLEX lentivirus backbone expresses mEmerald tagged ANXA11 R235Q mutant under EF1a promoter

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

    Insert

    ANXA11 (ANXA11 Human)
    

    Use

    Lentiviral

    Tags

    mEmerald

    Expression

    Mutation

    R235Q

    Promoter

    EF1a

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKLV2-U6(BsmBI)-EF1a-BFP-Nkx2-5

    Plasmid

    #117135

    Purpose

    Expression vector for Nkx2-5 and gRNA

    Depositor

    Kosuke Yusa

    Article

    Yu et al Stem Cells. 2019 Apr 1. doi: 10.1002/ste

    Insert

    Nkx2-5 CDS (Nkx2-5 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    modified BFP

    Promoter

    human U6 promoter, human EF1a promoter

    Available since

    April 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV-EF1a-KIF5A-HA-IRES-Puro

    Plasmid

    #166952

    Purpose

    Lentiviral plasmid expressing HA-tagged KIF5A protein with IRES-Puro from the EF1a promoter

    Depositor

    Rosalind Segal

    Article

    Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

    Insert

    KIF5A (Kif5a Mouse)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    eF1a

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCF824_U6-Sau-sgRNA-EF1a-mCherry2_lenti

    Plasmid

    #138318

    Purpose

    Lenti expression of mCherry2 with U6 promoter for SauCas9 sgRNA targeting GFP

    Depositor

    Jennifer Doudna

    Article

    Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

    Insert

    SauCas9 GFP sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHAGE2-EF1a-MCS-hPOT1_K90E_IRES-blast

    Plasmid

    #166412

    Purpose

    express hPOT1 K90E in mammalian cells

    Depositor

    Agnel Sfeir

    Article

    Pinzaru et al Genes Dev. 2020 Dec 1;34(23-24):1619-163

    Insert

    hPOT1 K90E (POT1 Human)
    

    Use

    Lentiviral

    Tags

    MYC

    Expression

    Mammalian

    Mutation

    K90E

    Promoter

    EF-1a

    Available since

    May 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. KO701: pMVP (L3-L2) HA tag + pA; EF1a::TETa-pA

    Plasmid

    #121806

    Purpose

    pMVP L3-L2 entry plasmid, contains HA tag-polyA + EF1a-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream EF1a-driven TETa

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    HA epitope tag-polyA + EF1a::TETa-polyA
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRRLNeo-pEF1a-p53ashL344P-mKate2-splitmVenusN

    Plasmid

    #69586

    Purpose

    Expresses mutated p53-L344P tagged with mKate2 and split N-term mVenus

    Depositor

    Galit Lahav

    Article

    Gaglia et al Mol Syst Biol. 2014 Oct 24;10:753. doi:

    Insert

    p53-L344P (TP53 Human)
    

    Use

    Lentiviral

    Tags

    mKate2 and split mVenus - N term

    Expression

    Mammalian

    Mutation

    p53 L344P mutation that prevents dimerization and…

    Promoter

    EF1alpha

    Available since

    April 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-EF1a-CasRx-T2A-mCherry-pA

    Plasmid

    #192484

    Purpose

    To express CasRx

    Depositor

    Jonathan Ploski

    Article

    Back et al Mol Ther Methods Clin Dev, 20 Aug 2025,

    Insert

    CasRx
    

    Use

    AAV

    Tags

    Expression

    Mutation

    No

    Promoter

    Ef1a

    Available since

    Aug. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. EF1a-mRuby2(miRE.FF4)-TS.FF4x4-bGH

    Plasmid

    #235295

    Purpose

    ComMAND closed-loop circuit regulating mRuby2 with 4 copies of microRNA target sites

    Depositor

    Kate Galloway

    Article

    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

    Insert

    mRuby2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a (human EF1a)

    Available since

    Aug. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEF1a mNeonGreen-3K-B1-10-IRES-mKate2-2A-PuroR

    Plasmid

    #215377

    Purpose

    Lentiviral expression of a cytosolic-localized split mNeonGreen variant that enables efficient complementation when the missing beta strand is present. mKate2 is present as a transduction control.

    Depositor

    Franz-Ulrich Hartl

    Insert

    mNeonGreen-3K-1-10
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLV EF1alpha INTS4 IRES Cherry

    Plasmid

    #232109

    Purpose

    Lentiviral expression of human INTS4

    Depositor

    Tom Misteli

    Article

    Sood et al Mol Cell. 2025 Mar 6;85(5):913-928.e8. d

    Insert

    INTS4 (INTS4 Human)
    

    Use

    Lentiviral

    Tags

    IRES CHERRY

    Expression

    Mammalian

    Mutation

    Wild Type

    Promoter

    EF 1alpha

    Available since

    Aug. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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