We narrowed to 16,198 results for: grna

1. pCRIS

   Plasmid

   #120424

   Purpose

   This plasmid encodes the complete CRISPR/dCas9 machinery for repressing transposition of the following bacterial Insertion Sequences: IS1, IS3, IS5, and IS150.

   Depositor

   Tamás Fehér

   Article

   Nyerges et al Synth Biol (Oxf). 2019;4(1):ysz008. doi:

   Insert

   CRISPR spacers targeting IS1, IS5, IS3, IS150
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   constitutive

   Available Since

   Jan. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAf-CRISPR-yA

   Plasmid

   #191015

   Purpose

   CRISPR vector based on Aspergillus flavus U6 promoter and terminator for targeting the yA gene, which contains AMA1(the HindIII-PstI fragment) and ptrA selection marker.

   Depositor

   Perng-Kuang Chang

   Article

   Chang et al Microbiol Spectr. 2023 Feb 14;11(1):e046

   Insert

   yA
   

   Use

   CRISPR

   Promoter

   Aspergillus flavus U6

   Available Since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRRL-mU6-GFPGO-IRES-mScarletI

   Plasmid

   #136895

   Purpose

   Lentivirus for base editing activatable GFP expression in mammalian cells. All-in-one vector with sgGO2.

   Depositor

   Lukas Dow

   Article

   Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

   Insert

   GFPGO-IRES-mScarletI
   

   Use

   Lentiviral

   Tags

   3xNLS on mScarlet

   Mutation

   ATG>ACG

   Promoter

   SFFV

   Available Since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDECKO_mCherry

   Plasmid

   #78534

   Purpose

   Backbone to clone single or paired sgRNAs

   Depositor

   Roderic Guigo, Rory Johnson

   Article

   Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   U6 (for expressing sgRNA)

   Available Since

   July 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAS088

   Plasmid

   #211502

   Purpose

   AAV genome backbone for AAV-Perturb-seq with direct gRNA capture and nuclei sorting based on eGFP.

   Depositor

   Randall Platt

   Article

   Santinha et al Nature. 2023 Oct;622(7982):367-375. doi:

   Type

   Empty backbone

   Use

   AAV, CRISPR, Cre/Lox, and Mouse Targeting

   Expression

   Mammalian

   Available Since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRRL-mU6-GFPGO-IRES-mScarletI-PGK-Neo

   Plasmid

   #136897

   Purpose

   Lentivirus for base editing activatable GFP expression in mammalian cells. All-in-one vector with sgGO.

   Depositor

   Lukas Dow

   Article

   Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

   Insert

   GFPGO-IRES-mScarletI
   

   Use

   Lentiviral

   Tags

   3xNLS on mScarlet

   Mutation

   ATG>ACG

   Promoter

   SFFV

   Available Since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTRIPZ TREX1-KI

   Plasmid

   #127701

   Purpose

   For knocking in TREX1 in mouse - Doxycyclin inducible

   Depositor

   Sandra Demaria

   Article

   Vanpouille-Box et al Nat Commun. 2017 Jun 9;8:15618. doi: 10.

   Insert

   mouse TREX1 cDNA (Trex1 Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Sept. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYPQAT10

   Plasmid

   #223382

   Purpose

   T-DNA vector for SpRY mediated mutagenesis for monocot plants; NG or NA PAM preference; SpRY was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; Hygromycin for plants selection.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   ZmUbi-SpRY-OsU3-gRNA scaffold
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Available Since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJMP8835

   Plasmid

   #220902

   Purpose

   MobileCRiSPRi test system encoded on a kanR Tn7 transposon; mScarlet-I, PD-sgRNA (mscar targeting), and PG-dCas9-novo

   Depositor

   Jason Peters

   Article

   Hall et al Appl Environ Microbiol. 2024 Sep 26:e003

   Insert

   dCas9-novo
   

   Use

   CRISPR

   Tags

   3xMyc

   Expression

   Bacterial

   Mutation

   D10A, H840A

   Available Since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. p8092 LentiCRISPR v2 sgNT-1

    Plasmid

    #163315

    Purpose

    Expresses Cas9 and a non-targeting control guide RNA

    Depositor

    Elizabeth White

    Article

    Hatterschide et al J Virol. 2020 Aug 17;94(17). pii: JVI.01

    Insert

    sgNT-1
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTC223

    Plasmid

    #70019

    Purpose

    Cas9/sgRNA targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based on Bean Yellow Dwarf Virus; sgRNA= 7

    Depositor

    Daniel Voytas

    Article

    Cermak et al Genome Biol. 2015 Nov 6;16(1):232. doi:

    Insert

    Nuclease (Cas9/sgRNA) + Donor + GVR
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Dec. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. TLCV2-sgHPDL #3

    Plasmid

    #174165

    Purpose

    knock out HPDL in human cell lines

    Depositor

    Michael Pacold

    Article

    Banh et al Nature. 2021 Sep 1. pii: 10.1038/s41586-

    Insert

    sgRNA against HPDL
    

    Use

    Lentiviral

    Available Since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. TLCV2-sgHPDL #9

    Plasmid

    #174166

    Purpose

    knock out HPDL in human cell lines

    Depositor

    Michael Pacold

    Article

    Banh et al Nature. 2021 Sep 1. pii: 10.1038/s41586-

    Insert

    sgRNA against HPDL
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLKO.1-shCtnnd2.1-GFP

    Plasmid

    #209095

    Purpose

    GFP expressing shRNA targeting Ctnnd2 N-terminus

    Depositor

    Cagla Eroglu

    Article

    Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

    Insert

    shCtnnd2.1
    

    Available Since

    Dec. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAS006

    Plasmid

    #211506

    Purpose

    AAV genome backbone with a CROP-seq-like design for indirect capture of gRNA sequences

    Depositor

    Randall Platt

    Article

    Santinha et al Nature. 2023 Oct;622(7982):367-375. doi:

    Type

    Empty backbone

    Use

    AAV, CRISPR, Cre/Lox, and Mouse Targeting

    Expression

    Mammalian

    Available Since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. PLKO.1-G3BP1-CDS

    Plasmid

    #136039

    Purpose

    G3BP1 shRNA (Targeting CDS) inserted into the PLKO.1 plasmid (CGGGAATTTGTGAGACAGTAT)

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Insert

    G3BP1 (G3BP1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDD428

    Plasmid

    #202081

    Purpose

    Cas9/sgRNA plasmid targeting the C-terminus of Myh9

    Depositor

    Daniel Dickinson

    Article

    Shi et al Development. 2023 May 15;150(10):dev2013

    Insert

    pEF1a-mCherry-Cas9 + Myh9 CT sgRNA (Myh9 S. pyogenes (Cas9))
    

    Use

    CRISPR

    Promoter

    EF1a

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. PX552-mScn2a-3xV5-EF1-smFP-flag

    Plasmid

    #182561

    Purpose

    For mouse Nav1.2 knockin with 3xV5 at the C-terminal

    Depositor

    James Zhe Liu

    Article

    Liu et al J Neurosci. 2022 Jun 6;42(28):5482-98. d

    Inserts

    mouse Scn2a gRNA and 3xV5 donor DNA
    smFP-flag
    

    Use

    AAV and CRISPR

    Promoter

    EF1 and U6

    Available Since

    March 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pYPQAT11

    Plasmid

    #223383

    Purpose

    T-DNA vector for SpRY mediated mutagenesis for monocot plants; NG or NA PAM preference; SpRY was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; BASTA for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-SpRY-OsU3-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Available Since

    July 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pYPQAT08

    Plasmid

    #223380

    Purpose

    T-DNA vector for SpRY mediated mutagenesis for dicot plants; NG or NA PAM preference; SpRY was driven by ZmUbi1 and the sgRNA was driven by AtU3 promoter; BASTA for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-SpRY-AtU3-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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