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Showing: 2201 - 2240 of 22214 results

  4. pASKt15C+SrtA7

    Plasmid
    #64986
    Purpose
    Expresses the P94R/E105K/E108A/D160N/D165A/K190E/K196T mutant of Staphylococcus aureus sortase A in E. coli
    Depositor
    Teruyuki Nagamune
    Article
    Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/
    Insert
    sortase A
    Use
    Tags
    His6 tag and S tag
    Expression
    Bacterial
    Mutation
    deleted amino acids 1-59, P94R, E105K, E108A, D16…
    Promoter
    tetracycline promoter/operator
    Available since
    June 9, 2015
    Availability
    Academic Institutions and Nonprofits only
    64986-plasmid-map-sequence-id-109956

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  8. mOtop1_pGEMHE

    Plasmid
    #114674
    Purpose
    This construct was used to express mouse Otop1 in Xenopus laevis oocytes
    Depositor
    Emily Liman
    Article
    Tu et al Science. 2018 Mar 2;359(6379):1047-1050.
    Insert
    Otopetrin 1 (Otop1 Mouse)
    Use
    For generating the mrna, which can highly express…
    Tags
    Expression
    Mutation
    SNP (rs49325632): Glycine 396 to Alanine
    Promoter
    T7
    Available since
    July 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    114674-plasmid-map-sequence-id-226716

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  16. pMpGWB316

    Plasmid
    #68644
    Purpose
    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants
    Depositor
    Takayuki Kohchi
    Article
    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do
    Type
    Empty backbone
    Use
    Tags
    Nuclear localization signal-tagged tdTomato (tdTo…
    Expression
    Plant
    Mutation
    Promoter
    Available since
    June 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    68644-plasmid-map-sequence-id-121804

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  18. pSAG1::CAS9-U6::sg290860-6

    Plasmid
    #59855
    Purpose
    Encodes CAS9 nuclease (GFP fusion) under control of the Toxoplasma gondii SAG1 promotor. Vector also provides U6 controlled expression of a single guide RNA for CRISPR disruption of TGME49_290860.
    Depositor
    David Sibley
    Article
    Behnke et al Eukaryot Cell. 2014 Dec 5. pii: EC.00229
    Insert
    CRISPR sg290860-6
    Use
    CRISPR; ; toxoplasma gondii
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    59855-plasmid-map-sequence-id-94714

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  19. pGG184

    Plasmid
    #165604
    Purpose
    Reporter plasmid for B1H-dependent expression of the HIS3/GFP operon with a single hEGFP protospacer: PS1(upstream of promoter with 'CAGCG' PAM)-lac-HIS3 and GFP
    Depositor
    Marcus Noyes
    Article
    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:
    Insert
    hEGFP protospacer ('CAGCG' PAM) upstream of the HIS3/GFP promoter
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    'CAGCG' PAM replacing the 'CGGCG&#…
    Promoter
    lac
    Available since
    June 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    165604-plasmid-map-sequence-id-334628

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  39. SI16-Kit a

    Plasmid
    #58948
    Purpose
    expresses recombinant mouse anti-Kit a receptor (zebrafish) IgG1 antibody in mammalian cells
    Depositor
    Gavin Wright
    Article
    Crosnier et al BMC Biol. 2010 . 8():76.
    Insert
    Kit receptor a mouse monoclonal antibody (kita Zebrafish)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 19, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 2201 - 2240 of 22214 results