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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 6,562 results for: nls

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Showing: 1101 - 1120 of 6562 results

  1. NESRA-DEVD-BNLS

    Plasmid
    #50849
    Purpose
    Expresses a tandem red ddFP heterodimer in mammalian cells, construct 1 for a red to green colour switch-based fluorescent caspase-3 biosensor, used together with a second plasmid GANLS.
    Depositor
    Robert Campbell
    Publication
    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ddRFP A and ddRFP B
    Tags
    NES sequence LQKKLEELELDE placed after ddGFP A an…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Jan. 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    50849-plasmid-map-sequence-id-76294

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  3. pDestTol2CR-QUAS:mNeonGreen-NES-P2A-mKate2-NLS

    Plasmid
    #241912
    Purpose
    Zebrafish transgenesis plasmid encoding cytoplasmic mNeonGreen-NES and mKate2-NLS separated by the P2A peptide, driven by the QUAS enhancer, and including a cmlc2:mKate2 cardiac transgenesis marker
    Depositor
    Balázs Enyedi
    Publication
    Fazekas et al J Cell Biol. 2026 Jan 5;225(1):e20250116
    Insert
    QUAS enhancer driving mNeonGreen-NES and mKate2-NLS
    Use
    Zebrafish tol2 transgenesis
    Promoter
    QUAS
    Available since
    Oct. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    241912-plasmid-map-sequence-id-487154

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  4. pcDNA3-His:dCas9:nls:10P3

    Plasmid
    #135982
    Purpose
    Expresses dCas9 fused to 10 copies of the P3 coiled coil in mammalian cells
    Depositor
    Roman Jerala
    Publication
    Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9 fused to 10 copies of the P3 coiled-coil
    Use
    CRISPR
    Tags
    His
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    CMV
    Available since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    135982-plasmid-map-sequence-id-308932

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  5. pDSP32

    Plasmid
    #199374
    Purpose
    Encodes dual cistronic nuclear localized jGCaMP7s and mScarlet-I optimized for C. elegans expression
    Depositor
    Hang Lu
    Insert
    NLS::jGCaMP7s::egl-13NLS::SL2::NLS::mScarlet-I::egl-13NLS
    Expression
    Bacterial
    Mutation
    no mutations, except added subcellular localizati…
    Available since
    April 25, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    199374-plasmid-map-sequence-id-390801

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  6. pCMV-Target-ACEmax

    Plasmid
    #139105
    Purpose
    Mammalian Target-ACEmax expression vector (pSI-625)
    Depositor
    Nozomu Yachie
    Publication
    Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Bi_partite_NLS-TadA*-XTEN_linker-TadA-XTEN_linker-SpCas9(D10A)-SH3_linker-PmCDA1-UGI-SV40NLS
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CMV promoter
    Available since
    June 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    139105-plasmid-map-sequence-id-273275

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  7. pCS2+ GCamp6-3NLS

    Plasmid
    #165401
    Purpose
    Expresses a nuclear localized genetically encoded calcium reporter.
    Depositor
    Philipp Niethammer
    Publication
    Enyedi et al Cell. 2016 May 19;165(5):1160-1170. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Nuclear Localization Signal
    Use
    Avian, xenopus, zebrafish
    Tags
    GCamp6
    Expression
    Mammalian
    Promoter
    SP6
    Available since
    March 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    165401-plasmid-map-sequence-id-325983

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  9. pNB774

    Plasmid
    #60114
    Purpose
    Green beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA)
    Depositor
    Nicolas Buchler
    Publication
    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GrLuc
    Use
    Unspecified
    Tags
    ClpXP degron and Nuclear localization signal (NLS…
    Available since
    Oct. 31, 2014
    Availability
    Academic Institutions and Nonprofits only
    60114-plasmid-map-sequence-id-96175

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  11. GANES-DEVD-BNLS

    Plasmid
    #50842
    Purpose
    Expresses a tandem green ddFP heterodimer in mammalian cells, construct 1 for a green to red colour switch-based fluorescent caspase-3 biosensor, used together with a second plasmid RANLS.
    Depositor
    Robert Campbell
    Publication
    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ddGFP A and ddGFP B
    Tags
    NES sequence LALKLAGLDIGS placed after ddGFP A an…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Jan. 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    50842-plasmid-map-sequence-id-76290

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  17. MTK3_009

    Plasmid
    #123724
    Purpose
    Encodes nuclear localized PHYB fused to activating domain of VP16 as Type 3 part to be used in the MTK system
    Depositor
    Hana El-Samad
    Publication
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PHYB-VP16-NLS
    Expression
    Mammalian
    Available since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    123724-plasmid-map-sequence-id-248657

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Showing: 1101 - 1120 of 6562 results