We narrowed to 92,654 results for: lias

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pCMV_UdgX-APOBEC1-UdgX-HF-nCas9

Plasmid

#163559

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

UdgX-APOBEC1-UdgX-HF-nCas9

Use

CRISPR

Expression

Mammalian

Mutation

nCas9 (D10A)HF (N497A, R661A, Q695A, Q926A)

Available Since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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Mini-Ii-Str_SBP-EGFP-LIMP2

Plasmid

#222324

Purpose

Synchronize the trafficking of LIMP2 from the ER.

Depositor

Franck Perez

Article

Ecard et al Mol Biol Cell. 2024 Mar 1;35(3):ar42. do

Insert

Streptavidin-KDEL and LIMP2 fused to SBP-EGFP (SCARB2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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CRY2olig-mCh-Nck

Plasmid

#60034

Purpose

Expresses a fusion containing CRY2olig (PHR, E490G) with mCherry and 3 SH3 domains of Nck

Depositor

Chandra Tucker

Article

Taslimi et al Nat Commun. 2014 Sep 18;5:4925. doi: 10.

Insert

CRY2-mCh-NCK (CRY2 Human, Mustard Weed)

Tags

HA tag

Expression

Mammalian

Mutation

M354I, E490G CRY2

Promoter

CMV

Available Since

Nov. 12, 2014

Availability

Academic Institutions and Nonprofits only

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DRH323: pAAV_hSyn-QuasAr2

Plasmid

#107705

Purpose

in vivo voltage imaging

Depositor

Adam Cohen

Article

Adam et al Nature. 2019 May 1. pii: 10.1038/s41586-

Insert

QuasAr2

Use

AAV

Expression

Mammalian

Available Since

May 2, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV.hSyn1.Twitch2B.WPRE.SV40

Plasmid

#100040

Purpose

AAV expression of Twitch2B driven from hSyn1 promoter. Twitch 2B is a fluorescent reporter for calcium signaling

Depositor

Oliver Griesbeck

Article

Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

Has Service

AAV1

Insert

Twitch2B

Use

AAV

Expression

Mammalian

Promoter

hSyn1

Available Since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-hA3A-BE3-Y130F

Plasmid

#113428

Purpose

Expresses hA3A-BE3-Y130F in mammalian cells

Depositor

Jia Chen

Article

Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

Insert

hA3A-BE3-Y130F (APOBEC3A S. pyogenes and Bacteriophage PBS2, Human)

Use

CRISPR

Expression

Mammalian

Mutation

hAPOBEC3A_Y130F

Promoter

CMV

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-hA3A-BE3-D131Y

Plasmid

#113424

Purpose

Expresses hA3A-BE3-D131Y in mammalian cells

Depositor

Jia Chen

Article

Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

Insert

hA3A-BE3-D131Y (APOBEC3A S. pyogenes and Bacteriophage PBS2, Human)

Use

CRISPR

Expression

Mammalian

Mutation

hAPOBEC3A_D131Y

Promoter

CMV

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pTol2-CreLite

Plasmid

#131783

Purpose

Tol2 destination vector carrying Ubi:CreLite with mTagBFP2 as a visual marker.

Depositor

George Eisenhoffer

Article

Yen et al Dev Dyn. 2020 Nov;249(11):1394-1403. doi

Insert

PhyBCreC, PIF6CreN, and mTagBFP2

Use

Cre/Lox and Synthetic Biology; Zebrafish tol2 tra…

Tags

mTagBFP2

Expression

Mammalian

Promoter

Ubi

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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nLYN267-gs10-NLS-VPR

Plasmid

#188265

Purpose

Plasmid ensures constitutive expression of the N-terminal fragment of split LYN kinase domain (aa 268-512), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminus

Depositor

Roman Jerala

Article

Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038

Insert

nLYN267 (LYN Human)

Expression

Mammalian

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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human CD40 promoter

Plasmid

#53433

Purpose

promoter activity study

Depositor

Etty Benveniste

Article

Nguyen et al J Biol Chem. 2002 Apr 19;277(16):13796-8

Insert

CD40 promoter (CD40 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Promoter

CD40

Available Since

May 29, 2014

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv1_SDHA

Plasmid

#177980

Purpose

lentiviral vector expressing Cas9 and a sgRNA targeting SDHA

Depositor

David Sabatini, Jessica Spinelli

Article

Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

Insert

sgRNA targeting SDHA (SDHA Human)

Use

Lentiviral

Expression

Mammalian

Mutation

N/A

Promoter

U6

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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(364) SB KRAB-SadCas9

Plasmid

#163022

Purpose

Sleeping Beauty TET-On expression of CRISPRi (without U6-gRNA)

Depositor

Tonia Rex

Article

Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

Insert

Mammalian codon-optimized SadCas9

Use

Transposon

Tags

KRAB and myc NLS

Expression

Mammalian

Promoter

TET-On

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/NS5-YFP-NLS-mut

Plasmid

#120832

Purpose

Ectopic expression of ZIKV protein

Depositor

Jan Rehwinkel

Article

Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

Insert

ZIKV NS5-eYFP "NLS-mut"

Tags

eYFP-V5

Expression

Mammalian

Mutation

R372A/Q373A/K387A/H388A/K389A

Promoter

CMV

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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SspB-HaloTag-DHPH-TIAM1

Plasmid

#176114

Purpose

Heterodimerization with iLID, Rac1 activation

Depositor

Dorus Gadella

Article

Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

Insert

TIAM1 (TIAM1 Human)

Expression

Mammalian

Available Since

Oct. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-IV-GFPL10

Plasmid

#98704

Purpose

Cre-dependently express GFPL10 fusion using Introvert Switch

Depositor

Jeffrey Friedman, Alexander Nectow

Article

Nectow et al Cell. 2017 Jul 27;170(3):429-442.e11. do

Insert

GFPL10

Use

AAV and Cre/Lox

Tags

GFP

Expression

Mammalian

Mutation

L10: 2609 C->T to remove EcoRI site

Promoter

EF1a

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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Cav2.2 BBS pcDNA3

Plasmid

#206057

Purpose

expression of rabbit Cav2.2 calcium channel with an exofacial double Bungarotoxin Binding Site motif in domain II

Depositor

Annette Dolphin

Article

Cassidy et al Proc Natl Acad Sci U S A. 2014 Jun 17;11

Insert

cacna1b (CACNA1B Rabbit)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pAY19 (OsTIR1(F74A) mAID-EGFP-NLS)

Plasmid

#140535

Purpose

OsTIR1(F74A)-P2A-mAID-EGFP-NLS TOL2

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

mAID-EGFP-NLS TOL2

Expression

Mammalian

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pMSCV neo WT Akt1

Plasmid

#14549

Depositor

Joan Brugge

Article

Irie et al J Cell Biol. 2005 Dec 19. 171(6):1023-34

Insert

Akt1 (Akt1 Mouse)

Use

Retroviral

Tags

HA tag

Expression

Mammalian

Available Since

April 12, 2007

Availability

Academic Institutions and Nonprofits only
pGP18 (CK2alpha' K69M)

Plasmid

#27090

Depositor

David Litchfield

Article

Turowec et al Methods Enzymol. 2010;484:471-93.

Insert

CK2alpha' K69M (CSNK2A2 Human)

Tags

HA

Expression

Mammalian

Mutation

K69M

Promoter

CMV

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3-FLAG -YWAA-pGK-HYG

Plasmid

#107381

Purpose

Mammalian expression of FLAG-YWAA

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

cereblon (CRBN Human)

Tags

FLAG

Expression

Mammalian

Mutation

CRBN-Y383A/W385A

Promoter

CMV

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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