Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 12,983 results for: NUC

Clear keywords
Showing: 11241 - 11260 of 12983 results

  1. pHS1742

    Plasmid
    #239838
    Purpose
    OrufIscB-KRK mammalian expression
    Depositor
    Feng Zhang
    Article
    Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038
    Insert
    OrufIscB-KRK
    Tags
    Nucleoplasmin NLS-3xHA and SV40 NLS
    Expression
    Mammalian
    Mutation
    Insertion of REC domain from NbaCas9-1 + E137K/E4…
    Promoter
    CMV
    Available Since
    July 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    239838-plasmid-map-sequence-id-479115

    Enlarge

  3. pLentiRNACRISPR_012-TetO-NLS-inactive-hfCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast

    Plasmid
    #228558
    Purpose
    Expresses inactive hfCas13d in mammalian cells for binding of target RNAs in combination with compatible crRNAs. Localized to the nucleus
    Depositor
    Neville Sanjana
    Article
    Hart et al Nat Biotechnol. 2025 Feb 11. doi: 10.103
    Insert
    dhfCas13d (N2V8)
    Use
    Lentiviral
    Tags
    NLS-HA
    Mutation
    R239A/H244A/R858A/H863A
    Available Since
    Aug. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    228558-plasmid-map-sequence-id-457180

    Enlarge

  7. pBP-T7_RBS-His6-Thrombin

    Plasmid
    #72979
    Purpose
    T7 promoter, RBS, His6-tag and thrombin cleave site - derived from pET15b (5' CTAT/3' CATA) fusion)
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    T7+RBS+His6+thrombin
    Use
    Synthetic Biology
    Mutation
    CAT gene - C435G (nucleotide) - silent mutagenesi…
    Available Since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only

  13. pSLQ10531

    Plasmid
    #239267
    Purpose
    Expresses NLS-dCas13b-ABI for CRISPR-TO perturbation in the nucleus of cell lines
    Depositor
    Stanley Qi
    Article
    Han et al Nature. 2025 Jul;643(8070):241-251. doi:
    Insert
    NLS-dPspCas13b-EGFP-ABI
    Use
    CRISPR and Lentiviral
    Tags
    Flag tag and V5 tag
    Expression
    Mammalian
    Mutation
    H133A and H1058A in PspCas13b
    Promoter
    EF1a
    Available Since
    Sept. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    239267-plasmid-map-sequence-id-475551

    Enlarge

  16. psiCHECK2-let-7 MT

    Plasmid
    #78261
    Purpose
    psiCHECK2 dual luciferase reporter harboring a mutated let-7 target element, cloned into the XhoI/NotI restriction sites, the 3'UTR of the Renilla Luciferase gene
    Depositor
    Joanne Weidhaas
    Article
    Salzman et al Nat Commun. 2016 Mar 21;7:10954. doi: 10
    Insert
    mutated let-7 target site
    Use
    Luciferase; Microrna activity
    Expression
    Mammalian
    Mutation
    nucleotides 2-5 and 10-11 changed from CTCC to GA…
    Available Since
    June 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    78261-plasmid-map-sequence-id-149478

    Enlarge

Showing: 11241 - 11260 of 12983 results