We narrowed to 84,770 results for: ell
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Plasmid#145030PurposepiggyBac vector for expressing a Turquoise calcium sensor (GECI) that reports with lifetime and intensity changesDepositorInsert3xnls-Tq-Ca-FLITS
Tags3xnls-Tq-Ca-FLITSExpressionMammalianPromoterCMVAvailable SinceJune 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLX304 3xFlag-eRF1 G183/184A_IRES-GFP
Plasmid#198394PurposeeRF1 (AAQ mutant) expressionDepositorInserteRF1 (ETF1 Human)
UseLentiviralTags3xFlagExpressionMammalianMutationmutated GGQ motif (G183/184A)PromoterCMVAvailable SinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
vYM133_PB-pEF-osTIR1-T2A-iaaH-T2A-mGFPmut3-mAID-iCasp9-T2A-mCh-AID-BlastR
Plasmid#160046PurposeFor expressing the full paradoxical circuitDepositorInsertsosTIR1
iaaH
iCasp9
BlastR
UseSynthetic BiologyTagsmAID, mCherry, and mGFPmut3ExpressionMammalianPromoterno promoter, T2A is used and pEF1sAvailable SinceApril 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEF1a-hMLH1dn (og codon)
Plasmid#174823PurposeMammalian expression of human MLH1dn (original codon usage)DepositorInserthuman MLH1dn (original codon usage) (MLH1 Human)
ExpressionMammalianMutationdeletion of residues 754-756PromoterEF1aAvailable SinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
Tet-O-FUW-EGFP-P2A-mCherry
Plasmid#203868PurposeTet-inducible lentiviral plasmid expressing EGFP-P2A-mCherry, with ORF specific barcode close to 3'LTR siteDepositorInsertEGFP
ExpressionMammalianAvailable SinceAug. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLentiDP1.3
Plasmid#171096Purposelentiviral construct (3rd generation) for the expression of cell cycle-dependent OsTIR1 fused with mEmerald and Cdt1 (AKA ROLECCS G1) and miniAID-fused mCherry2DepositorInsertOsTIR1-mEmerald-Cdt1-P2A-miniAID-mCherry2
UseLentiviral and Synthetic BiologyTagsEmerald, cdt1, miniAID, mCherry2ExpressionMammalianPromoterCMVAvailable SinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Puro-mStayGold-TUBA1B
Plasmid#227321PurposeDonor template for Puro-2A-mStayGold insertion into the N-terminus of the TUBA1B locus. For tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B (Addgene #207763)DepositorInsertTUBA1B Homology Arms flanking a Puro-2A-mStayGold Cassette (TUBA1B Human)
UseCRISPR; Donor templateExpressionMammalianPromoterPromoterlessAvailable SinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
PX-TIR-GFP
Plasmid#52737PurposeRetroviral vector expresses the lipid binding domain of p40-phox fused to the TIR of hTIRAP and GFP. Also contains IRES-hCD2 as selectable markerDepositorInsertToll/IL-1 Receptor adaptor protein (TIRAP Human)
UseRetroviralTagsGFP and p40 Phox lipid binding domainExpressionMammalianMutationDeleted amino acids 1-85Available SinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
PLC-TIR-GFP
Plasmid#52736PurposeRetroviral vector expresses the lipid binding domain of PLCd1 fused to the TIR of hTIRAP and GFP. Also contains IRES-hCD2 as selectable markerDepositorInsertToll/IL-1 Receptor adaptor protein (TIRAP Human)
UseRetroviralTagsGFP and PLCd1 Lipid bindingExpressionMammalianMutationDeleted amino acids 1-85Available SinceJune 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
LARG mCherry sspB in pcDNA3.1
Plasmid#90459PurposeRhoA mediated cell migrationDepositorInsertsExpressionMammalianMutationR73QPromoterCMVAvailable SinceJune 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
SLP2a-TIR-GFP
Plasmid#52738PurposeRetroviral vector expresses the lipid binding domain of SLP2a fused to the TIR of hTIRAP and GFP. Also contains IRES-hCD2 as selectable markerDepositorInsertToll/IL-1 Receptor adaptor protein (TIRAP Human)
UseRetroviralTagsC2A domain of Murine SLP2a and GFPExpressionMammalianMutationDeleted amino acids 1-85Available SinceJune 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLentiDP1.2
Plasmid#171095Purposelentiviral construct (3rd generation) for the expression of cell cycle-dependent OsTIR1 fused with mEmerald and Geminin (AKA ROLECCS G2) and miniAID-fused mCherry2DepositorInsertOsTIR1-mEmerald-Geminin-P2A-miniAID-mCherry2
UseLentiviral and Synthetic BiologyTagsEmerald, Geminin, miniAID, mCherry2ExpressionMammalianPromoterCMVAvailable SinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV[Exp]-Puro-EF-1α>TurboRFP(ns):T2A:{H2B-RFP}
Plasmid#201120PurposeRFP expression in cells under EF-1α promoter; Histone-bound, nuclearDepositorInsertTurboRFP; H2B-RFP
UseLentiviralMutationNoAvailable SinceMay 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
H2A-mTurquoise-CDC42-G12V-deltaCAAX
Plasmid#176094PurposeConstitutively active, nuclear localized Cdc42DepositorAvailable SinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-MYO10-HMM
Plasmid#87256PurposeExpression plasmid to perform NanoSPD assays in mammalian cells (generates EGFP-tagged bait).DepositorInsertMYO10 (Myo10 Mouse)
ExpressionMammalianMutationMyo10 is truncated to contain aa.1-941PromoterCMVAvailable SinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-Solo-mStayGold-MAPRE1
Plasmid#227324PurposeDonor template for mStayGold insertion into the C-terminus of the MAPRE1 locus. For growing microtubule tip visualization. To be co-transfected with sgRNA plasmid px330-PITCh-MAPRE1 (Addgene #207793)DepositorInsertMAPRE1 Homology Arms flanking a mStayGold Tag (MAPRE1 Human)
UseCRISPR; Donor templateExpressionMammalianPromoterPromoterlessAvailable SinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBK086
Plasmid#183145PurposepET-6xHis-MBP-TEV-MapTIR-APAZ/MapAgo - Heterolgous MapSPARTA expressionDepositorInsertMapSPARTA operon (6xHis-MBP-MapTIR-APAZ and MapAgo)
Tags6xHis and MBPExpressionBacterialPromoterT7Available SinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-GTF2IRD1__ALK
Plasmid#116821PurposeLentiviral expression of GTF2IRD1__ALK fusionDepositorInsertGTF2IRD1__ALK
UseLentiviralAvailable SinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-SEMA3F-COMP5AP-AviTag-9xHis
Plasmid#157541PurposeMammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.DepositorAvailable SinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBK092
Plasmid#183146PurposepET-6xHis-MBP-TEV-CrtTIR-APAZ/CrtAgo - Heterologous CrtSPARTA expressionDepositorInsertCrtSPARTA operon (6xHis-MBP-CrtTIR-APAZ and CrtAgo)
Tags6xHis and MBPExpressionBacterialPromoterT7Available SinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only