We narrowed to 13,293 results for: gnal

1. pWZLblasti-HA-ERK2 D321N

   Plasmid

   #53173

   Purpose

   Expresses HA tagged human ERK2 D321N from blasticidin resistance retroviral vector

   Depositor

   Christopher Counter

   Article

   Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

   Insert

   ERK2 (MAPK1 Human, Mustard Weed)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   D321N

   Promoter

   Available since

   June 24, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pAAV-UBC-S3-RAB_EKARev

   Plasmid

   #160727

   Purpose

   Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains RFP-based fluorescent reporter RAB-EKARev (ERK indicator), VSV-G tag, and S3 protein scaffold.

   Depositor

   Edward Boyden

   Article

   Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

   Insert

   S3-RAB_EKARev
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   Human ubiquitin C (UBC) promoter

   Available since

   Dec. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-UBC-S1-jRCaMP1a

   Plasmid

   #160728

   Purpose

   Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains RFP-based fluorescent reporter jRCaMP1a (calcium indicator), Xpress tag, and S1 protein scaffold.

   Depositor

   Edward Boyden

   Article

   Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

   Insert

   S1-jRCaMP1a
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   Human ubiquitin C (UBC) promoter

   Available since

   Dec. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLVX-CMV-YFP-CAAX-IRES*-GINIP-Nluc W139A

   Plasmid

   #223560

   Purpose

   Alias: Gαi bONE-GO W139A. Lentiviral vector expressing GINIP-Nluc W139A after a low efficiency IRES downstream of YFP-CAAX placed after the promotor

   Depositor

   Mikel Garcia-Marcos

   Article

   Luebbers et al Sci Signal. 2025 Mar 25;18(879):eadp6457

   Insert

   YFP(Venus) - KRas4b - IRES* - human GINIP W139A - linker(GGGS) - Nluc
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   W139A mutation in GINIP sequence

   Promoter

   CMV

   Available since

   March 28, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pCDNA3.1-SP-ALFA-lk-HiBiT-lk-ADGRL3-lk-HiBiT

   Plasmid

   #233064

   Purpose

   Expresses mouse ADGRL3 (UniProt Q80TS3-3). An N-terminal AFLA-tag (SRLEEELRRRLTE) and HiBiT tag (VSGWRLFKKIS) follow the endogenous ADGRL3 signal peptide. Also includes a C-terminal HiBiT tag.

   Depositor

   Jonathan Javitch

   Article

   Perry-Hauser et al J Biol Chem. 2025 Jan 9:108174. doi: 10.

   Insert

   ADGRL3 (Adgrl3 Mouse)
   

   Use

   Tags

   ALFA and HiBiT

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCDNA3.1-SP-ALFA-lk-HiBiT-lk-ADGRL3-T923G

   Plasmid

   #233063

   Purpose

   Expresses cleavage-deficient mouse ADGRL3-T923G (UniProt Q80TS3-3). An N-terminal AFLA-tag (SRLEEELRRRLTE) and HiBiT tag (VSGWRLFKKIS) follow the endogenous ADGRL3 signal peptide.

   Depositor

   Jonathan Javitch

   Article

   Perry-Hauser et al J Biol Chem. 2025 Jan 9:108174. doi: 10.

   Insert

   ADGRL3 (Adgrl3 Mouse)
   

   Use

   Tags

   ALFA and HiBiT

   Expression

   Mammalian

   Mutation

   Threonine 923 to Glycine

   Promoter

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMSCV-NLS-CBGreen-FIRE-puro

   Plasmid

   #139195

   Purpose

   Luminescent reporter of ERK activity

   Depositor

   Matthew Lazzara

   Article

   Day et al Cell Rep. 2020 Mar 10;30(10):3383-3396.e

   Insert

   CBG99
   

   Use

   Luciferase and Retroviral

   Tags

   Fra1 PEST domain fragment (FIRE) and nuclear loca…

   Expression

   Mammalian

   Mutation

   See depositor comments below

   Promoter

   Available since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Cx36-GCaMP

   Plasmid

   #123604

   Purpose

   Mouse Connexin 36-GCaMP3 fusion for measuring gap junction localized calcium signaling - expresses in mammalian cells

   Depositor

   John O'Brien

   Article

   Moore et al eNeuro. 2020 Mar 11. pii: ENEURO.0445-19

   Insert

   Connexin 36-GCaMP3 fusion (Gjd2 Mouse)
   

   Use

   Tags

   GCaMP3

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAL113

   Plasmid

   #22471

   Purpose

   Expresses N-terminal domains of PhyB (with Y276H mutation), fused to mCFP and HRas C terminal CAAX palmitoylation sequence. For red-light-induced binding to PIF6.

   Depositor

   Christopher Voigt

   Article

   Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

   Insert

   PhyB NT (PHYB Mouse, Mustard Weed)
   

   Use

   Synthetic Biology

   Tags

   10aaLinker-mCFP-HrasCT

   Expression

   Mammalian

   Mutation

   Y276H

   Promoter

   CMV

   Available since

   Nov. 5, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCCLc-MND-A0201-SABR-Backbone

    Plasmid

    #119050

    Purpose

    3rd gen transfer vector. HLA-A0201 linked to b2-microglobulin and CD28-CD3z signaling domain. Contains a stuffer fragment flanked by BsmBI sites at the epitope cloning site.

    Depositor

    David Baltimore

    Article

    Joglekar et al Nat Methods. 2019 Feb;16(2):191-198. doi

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    MND

    Available since

    Jan. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCCLc-MND-A0201-Mart1-SABR

    Plasmid

    #119052

    Purpose

    3rd gen transfer vector. HLA-A0201 linked to b2-microglobulin and CD28-CD3z signaling domain. Contains the Mart1 epitope "ELAGIGILTV" cloned via BsmBI

    Depositor

    David Baltimore

    Article

    Joglekar et al Nat Methods. 2019 Feb;16(2):191-198. doi

    Insert

    A0201-Mart1-SABR
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    MND

    Available since

    Jan. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3-HA-eEF2K

    Plasmid

    #110160

    Purpose

    Expression of human eEF2K in mammalian cells

    Depositor

    Daniele Guardavaccaro

    Article

    Kruiswijk et al Sci Signal. 2012 Jun 5;5(227):ra40. doi:

    Insert

    eEF2K (eukaryotic elongation factor 2 kinase) (EEF2K Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pg-EGFP-HIF-2alpha

    Plasmid

    #87207

    Purpose

    N-terminally EGFP-tagged human HIF-2a/EPAS

    Depositor

    Violaine Sée

    Article

    Bagnall et al J Biol Chem. 2014 Feb 28;289(9):5549-64.

    Insert

    HIF-2a (EPAS1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Q868E

    Promoter

    CMV

    Available since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCCLc-MND-B2705-SABR-Backbone

    Plasmid

    #119051

    Purpose

    3rd gen transfer vector. HLA-B2705 linked to b2-microglobulin and CD28-CD3z signaling domain. Contains a stuffer fragment flanked by BsmBI sites at the epitope cloning site.

    Depositor

    David Baltimore

    Article

    Joglekar et al Nat Methods. 2019 Feb;16(2):191-198. doi

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCCLc-MND-B2705-KK10-SABR

    Plasmid

    #119053

    Purpose

    3rd gen transfer vector. HLA-B2705 linked to b2-microglobulin and CD28-CD3z signaling domain. Contains the HIV(gag) KK10 epitope "KRWIILGLNK" cloned via BsmBI

    Depositor

    David Baltimore

    Article

    Joglekar et al Nat Methods. 2019 Feb;16(2):191-198. doi

    Insert

    B2705-KK10-SABR
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    MND

    Available since

    Jan. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAL130

    Plasmid

    #22475

    Purpose

    Expresses N terminal domain of PIF3 fused to mYFP, for red-light-induced binding to Phy

    Depositor

    Christopher Voigt

    Article

    Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

    Insert

    PIF3NT (PIF3 Mouse, Mustard Weed)
    

    Use

    Synthetic Biology

    Tags

    mYFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. p3xFLAG-CMV-Tlr4(Lps)

    Plasmid

    #27149

    Depositor

    Bruce Beutler

    Article

    Poltorak et al Science. 1998 Dec 11. 282(5396):2085-8.

    Insert

    toll-like receptor 4(Lps) (Tlr4 Mouse)
    

    Use

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    changed Proline 712 to Histidine. Native signal p…

    Promoter

    Available since

    Feb. 10, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pGEX6P1-HA-ERK5 K84R

    Plasmid

    #53171

    Purpose

    Expresses GST-HA tagged human ERK5 K84R from bacterial expression vector

    Depositor

    Christopher Counter

    Article

    Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

    Insert

    ERK5 (MAPK7 Human)
    

    Use

    Tags

    GST and HA

    Expression

    Bacterial

    Mutation

    K84R

    Promoter

    Available since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pCDNA3 T7 Jip1(delta JBD)

    Plasmid

    #51767

    Purpose

    " JIP-1 may suppress signal transduction by the JNK pathway"

    Depositor

    Roger Davis

    Article

    Dickens et al Science. 1997 Aug 1. 277(5326):693-6.

    Insert

    Jnk interacting protein 1 (Mapk8ip1 Mouse)
    

    Use

    Tags

    T7

    Expression

    Mammalian

    Mutation

    deleted 126-263

    Promoter

    Available since

    March 14, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pGEX-4T3-ZO-2-1st-PDZ-delta-NLS

    Plasmid

    #27425

    Depositor

    Marius Sudol

    Article

    Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

    Insert

    Zonula Occludens 2 (TJP2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Deletion of NLS-Nuclear Localization Signal: Nucl…

    Promoter

    Available since

    May 18, 2011

    Availability

    Academic Institutions and Nonprofits only