We narrowed to 59,253 results for: {{{s}}}

1. pNeuLite

   Plasmid

   #16247

   Depositor

   Mien-Chie Hung

   Article

   Xing et al Nat Med. 2000 Feb . 6(2):189-95.

   Insert

   Her-2/neu promoter (ERBB2 Human)
   

   Use

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 30, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSUMO-β′-COPI-WD40

   Plasmid

   #180753

   Purpose

   Bacterial expression plasmid for Saccharomyces cerevisiae β′-COPI-WD40 (residues 1-301).

   Depositor

   Syed Saif Hasan

   Article

   Dey et al Commun Biol. 2022 Feb 8;5(1):115. doi: 1

   Insert

   β′-COPI-WD40 (SEC27 Budding Yeast)
   

   Use

   Tags

   N-terminal strep tag, His-tag, and SUMO tag

   Expression

   Bacterial

   Mutation

   Encodes residues 1-301; deleted 302-889; mutated …

   Promoter

   T7

   Available Since

   March 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV-SI-121

   Plasmid

   #131126

   Purpose

   A-to-G base editing reporter

   Depositor

   Nozomu Yachie

   Article

   Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

   Insert

   mutant EGFP
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available Since

   Sept. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET48 AtGUN1-PS

   Plasmid

   #136358

   Purpose

   Bacterial expression of recombinant Arabidopsis thaliana GUN1 (amino acids 232 to 918)

   Depositor

   Tatsuru Masuda

   Article

   Shimizu et al Proc Natl Acad Sci U S A. 2019 Dec 3;116

   Insert

   cDNA of Arabidopsis thaliana GUN1 corresponding to amino acids 232 to 918 (GUN1 Mustard Weed)
   

   Use

   Tags

   TRX-His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Feb. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. eSpCas9-EF-5Nanog_2

   Plasmid

   #174871

   Purpose

   CRISPR vector for generating Nanog STREAMING-tag KI cell

   Depositor

   Hiroshi Ochiai

   Article

   Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

   Insert

   sgRNA for mouse Nanog (Nanog Synthetic)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. Flag Intersectin Long (human)

   Plasmid

   #47393

   Depositor

   Peter McPherson

   Article

   Hussain et al Nat Cell Biol. 2001 Oct;3(10):927-32.

   Insert

   intersectin long (ITSN1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 29, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pET-45b-mEGFP

   Plasmid

   #185013

   Purpose

   Bacterial Expression of 6xHis Sirius-GFP Fusion Protein

   Depositor

   Denes Hnisz

   Article

   Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

   Insert

   Sirius-GFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHEN2 F8-2

   Plasmid

   #186033

   Purpose

   Express VHH F8-2 with 3 c-myc tag in E. coli periplasm

   Depositor

   Isabelle Landrieu

   Article

   Danis et al Mol Ther. 2022 Apr 6;30(4):1484-1499. do

   Insert

   VHH F8-2
   

   Use

   Tags

   6His and Myc

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available Since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCEP4-myc-HLA-B15

   Plasmid

   #135513

   Purpose

   Mammalian expression of myc-tagged HLA-B*15:01

   Depositor

   Erik Procko

   Article

   McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

   Insert

   HLA-B*15:01 (HLA-B Human)
   

   Use

   Tags

   Extracellular c-myc epitope tag and Signal peptid…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. ER-CanlonicSF

    Plasmid

    #178343

    Purpose

    To explore endoplasmic reticulum lactate dynamics

    Depositor

    Alejandro San Martín

    Article

    Aburto et al ACS Sens. 2022 Oct 28. doi: 10.1021/acss

    Insert

    ER-CanlonicSF
    

    Use

    Tags

    ER Retention Signal, ER Signal Exon 1, and ER Sig…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSW646 - pML1>>

    Plasmid

    #115995

    Purpose

    destination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expression

    Depositor

    Sebastien Wolf

    Article

    Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

    Insert

    pML1:B-dummy:GR-LhG4:D-dummy:tRBCS:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
    

    Use

    Tags

    HDEL and SP(ER)

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. PLL5.0-Anillin ShRNA-Cherry-AnillinFL

    Plasmid

    #187271

    Purpose

    Lentiviral expression of shRNA targeting Anillin + reconstitution of Anillin full length

    Depositor

    Alpha Yap

    Article

    Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

    Insert

    Anillin ,hsAnillin, Entrez Gene ANLN (a.k.a. FSGS8, Scraps, scra) (ANLN Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    U6, CMV

    Available Since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLL5.0-Anillin shRNA

    Plasmid

    #187270

    Purpose

    Lentiviral expression of shRNA targeting Anillin

    Depositor

    Alpha Yap

    Article

    Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

    Insert

    shRNA for Anillin (ANLN Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    U6

    Available Since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. Cytosolic Fyn FRET Biosensor

    Plasmid

    #124654

    Purpose

    FRET biosensor. The highly sensitive biosensor has a mutated SH2 domain, ECFP and YPet

    Depositor

    Yingxiao Wang

    Article

    Ouyang et al ACS Sens. 2019 Jan 25;4(1):76-86. doi: 1

    Insert

    Synthetic gene containing ECFP-SH2 domain-substrate-linker-YPet
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCX0393

    Plasmid

    #205984

    Purpose

    Ga0116245_10002768 T7/LacO-Cas6 + Cas14-TwinStrep + Cas7 + His14-Cas5::pJ23119-truncated DR-truncated Fn spacer

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

    Inserts

    Ga0116245_10002768 candidate type VII proteins
    Cas14
    Cas5
    truncated CRISPR array
    

    Use

    Tags

    14xHis and TwinStrep-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 (proteins), pJ23119 (crRNA)

    Available Since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLEX_305-N-dTAG_OGT

    Plasmid

    #155196

    Purpose

    N-terminal FKBP12(F36V)-2xHA tag on lentiviral OGT construct

    Depositor

    Suzanne Walker

    Article

    Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

    Insert

    O-GlcNAc Transferase (OGT Human)
    

    Use

    Lentiviral

    Tags

    2x HA and FKBP12(F36V)

    Expression

    Mammalian

    Mutation

    lacking N-terminal Met

    Promoter

    Available Since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAFW-Ago2

    Plasmid

    #50554

    Purpose

    Expression of FLAG tagged Ago2 in S2 cell

    Depositor

    Yukihide Tomari

    Article

    Iwasaki et al Mol Cell. 2010 Jul 30;39(2):292-9. doi:

    Insert

    Argonaute2 (AGO2 Fly)
    

    Use

    Tags

    FLAG

    Expression

    Insect

    Mutation

    Promoter

    Actin5C

    Available Since

    Feb. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUltraI-Tet3.0[TAA]

    Plasmid

    #164580

    Purpose

    Machinery plasmid containing a single copy of the Mb Tet3.0RS and cognate tRNA for incorporation of Tet3.0 at TAA codons

    Depositor

    Ryan Mehl

    Article

    Bednar et al ACS Chem Biol. 2021 Nov 19;16(11):2612-2

    Inserts

    Mb Tet3.0RS (R2-3)
    Mm tRNA[TAA]
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    L170G, N311G, C313A

    Promoter

    ProK and TacI

    Available Since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHS1950

    Plasmid

    #205983

    Purpose

    Ga0116245_10002768 T7/LacO-Cas6 + TwinStrep-Cas14 + Cas7 + His14-Cas5::pJ23119-DR-Fn spacer-DR

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

    Inserts

    Ga0116245_10002768 candidate type VII proteins
    Cas14
    Cas5
    CRISPR array
    

    Use

    Tags

    14xHis and TwinStrep-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 (proteins), pJ23119 (crRNA)

    Available Since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pYPQ265E6

    Plasmid

    #213469

    Purpose

    Gateway entry clone (attL1 & attR5) TadA-CDa-32aa linker-zSpCas9-D10A-2xUGI for C-T base editing

    Depositor

    Yiping Qi

    Article

    Fan et al Nat Commun. 2024 Jun 14;15(1):5103. doi:

    Insert

    TadA-CDa-32aa linker-zSpCas9-D10A-2xUGI
    

    Use

    CRISPR; Gateway compatible tada-cda-32aa linker-z…

    Tags

    No

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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