We narrowed to 24,814 results for: CHI;

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p8215 pHAGE N-V5 PTPN14 ΔPPXY1/2

Plasmid

#221646

Purpose

Constitutive ectopic expression of human PTPN14 ΔPPXY1/2 mutant

Depositor

Elizabeth White

Article

Blakely et al mBio. 2024 Sep 9:e0181124. doi: 10.1128/

Insert

PTPN14 ΔPPXY1/2 (PTPN14 Human)

Use

Lentiviral

Tags

V5

Mutation

deletion of the first and second (tandem) PPXY mo…

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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p8180 pHAGE N-V5 PTPN14 ΔPPXY3/4

Plasmid

#221647

Purpose

Constitutive ectopic expression of human PTPN14 ΔPPXY3/4 mutant

Depositor

Elizabeth White

Article

Blakely et al mBio. 2024 Sep 9:e0181124. doi: 10.1128/

Insert

PTPN14 ΔPPXY3/4 (PTPN14 Human)

Use

Lentiviral

Tags

V5

Mutation

deletion of the third and fourth PPXY motifs as d…

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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p8189 pHAGE N-V5 PTPN14 C1121S

Plasmid

#221648

Purpose

Constitutive ectopic expression of human PTPN14 C1121S mutant

Depositor

Elizabeth White

Article

Blakely et al mBio. 2024 Sep 9:e0181124. doi: 10.1128/

Insert

PTPN14 C1121S (PTPN14 Human)

Use

Lentiviral

Tags

V5

Mutation

C1121S mutation at the putative catalytic site

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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p8391 LentiCRISPRv2 Neo sgPTPN14-1

Plasmid

#221651

Purpose

Expression of sgRNA targeting the locus of human PTPN14

Depositor

Elizabeth White

Article

Blakely et al mBio. 2024 Sep 9:e0181124. doi: 10.1128/

Insert

sgPTPN14-1 (PTPN14 Human)

Use

CRISPR and Lentiviral

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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p8392 LentiCRISPRv2 Neo sgPTPN14-3

Plasmid

#221652

Purpose

Expression of sgRNA targeting the locus of human PTPN14

Depositor

Elizabeth White

Article

Blakely et al mBio. 2024 Sep 9:e0181124. doi: 10.1128/

Insert

sgPTPN14-3 (PTPN14 Human)

Use

CRISPR and Lentiviral

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-AP-SpyCatcher

Plasmid

#223636

Purpose

Protein purification of SpyCatcher003 (S49C) with 10xHis and AP fragment of split APEX2 tags from E. coli

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

SpyCatcher003 S49C

Tags

His10, AP fragment of split APEX2 tag

Expression

Bacterial

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-SpyCatcher -AVI

Plasmid

#223635

Purpose

Protein purification of SpyCatcher003 (S49C) with AVI and 6xHis tags from E. coli

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

SpyCatcher003 S49C

Tags

AVI tag and His6

Expression

Bacterial

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pRSET-Cm-CspA1

Plasmid

#215398

Purpose

Cm NCPPB382 cspA1 codon-optimized for E. coli recombinant protein expression (6xHis-CspA1)

Depositor

Gitta Coaker

Article

Stevens et al Proc Natl Acad Sci U S A. 2024 Jun 4;121

Insert

cspA1

Tags

6xHis Tag

Expression

Bacterial

Mutation

Codon-optimized for E. coli BL21(DE3)

Promoter

T7 promoter

Available Since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pBK1302-AAV-EFSNC-dCjCas9-KRAB

Plasmid

#223147

Purpose

Expression of KRAB with dCjCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

KRAB

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pBK1312-AAV-EFSNC-dSaCas9-KRAB

Plasmid

#223157

Purpose

Expression of KRAB with dSaCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

KRAB

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pRetro-UMI

Plasmid

#222686

Purpose

CRISPR-screening

Depositor

Ulrich Elling

Article

Michlits et al Nat Methods. 2017 Dec;14(12):1191-1197.

Type

Empty backbone

Use

CRISPR and Retroviral

Expression

Mammalian

Mutation

PGK without BbsI cutsite

Available Since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pGM258

Plasmid

#220176

Purpose

Expresses exogenous C-terminal mutant TTC1 (I271D)

Depositor

Jonathan Weissman

Article

Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

Insert

TTC1 (TTC1 Human)

Use

Lentiviral

Tags

3xFLAG

Mutation

I271D

Available Since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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pGM133

Plasmid

#220173

Purpose

CRISRPi guide RNA against TTC1

Depositor

Jonathan Weissman

Article

Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

Insert

TTC1 (sgRNA) (TTC1 Human)

Use

Lentiviral

Available Since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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BS-ZJLEU-PTEF1-HFL1-D384A Y387A-GFP

Plasmid

#207024

Purpose

Expression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

HFL1

Tags

GFP

Expression

Yeast

Mutation

D384A Y387A

Promoter

pTEF1

Available Since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

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BS-ZJLEU-PTEF1-HFL1-W371A I375A-GFP

Plasmid

#207023

Purpose

Expression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

HFL1

Tags

GFP

Expression

Yeast

Mutation

W371A I375A

Promoter

pTEF1

Available Since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

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BS-ZJLEU-PTEF1-HFL1-Y387A-GFP

Plasmid

#207022

Purpose

Expression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

HFL1

Tags

GFP

Expression

Yeast

Mutation

Y387A

Promoter

pTEF1

Available Since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

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BS-ZJLEU-PTEF1-HFL1-D384A-GFP

Plasmid

#207021

Purpose

Expression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

HFL1

Tags

GFP

Expression

Yeast

Mutation

D384A

Promoter

pTEF1

Available Since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

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BS-ZJLEU-PTEF1-HFL1-I375A-GFP

Plasmid

#207020

Purpose

Expression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

HFL1

Tags

GFP

Expression

Yeast

Mutation

I375A

Promoter

pTEF1

Available Since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

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BS-ZJLEU-PTEF1-HFL1-W371A-GFP

Plasmid

#207019

Purpose

Expression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

HFL1

Tags

GFP

Expression

Yeast

Mutation

W371A

Promoter

pTEF1

Available Since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

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Ypq2-2GFP-URA

Plasmid

#207010

Purpose

Integration of 2xGFP tag at YPQ2 C terminus, use auxotrophic marker URA3(Kluyveromyces lactis) for selection. Marker for yeast vacuolar membrane.

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

YPQ2

Tags

2xGFP

Expression

Yeast

Available Since

June 3, 2024

Availability

Academic Institutions and Nonprofits only

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