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Showing: 2281 - 2320 of 2376 results

  1. pET30a-His6-ABP-LOXCAT

    Plasmid
    #139971
    Purpose
    Fusion of A. viridans lactate oxidase (LOX) and E. coli catalase (CAT) that converts lactate into pyruvate without producing H2O2. Contains a albumin binding peptide (ABP).
    Depositor
    Vamsi Mootha
    Article
    Patgiri et al Nat Biotechnol. 2020 Jan 13. pii: 10.103
    Insert
    A fusion of lactate oxidase from A. viridans and catalase from E. coli
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Sept. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    139971-plasmid-map-sequence-id-311161

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  6. B52 + SPRTN sgSTOP

    Plasmid
    #100709
    Purpose
    B52 plasmid expressing SPRTN sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning)
    Depositor
    Alberto Ciccia
    Article
    Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17
    Insert
    sgSTOP targeting SPRTN (cloned using BbsI)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 promoters
    Available since
    Sept. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    100709-plasmid-map-sequence-id-193207

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  9. pDragon-Ctgf

    Plasmid
    #155015
    Purpose
    For Flp-mediated cassette exchange. Expresses tdTomato in the presence of (r)tTA, switching to connective tissue growth factor in the presence of Cre and (r)tTA activity.
    Depositor
    Alberto Rosello-Diez
    Article
    Ahmadzadeh et al Development. 2020 May 28;147(10). pii: d
    Insert
    Ccn2 (Ccn2 Mouse)
    Use
    Mouse Targeting
    Tags
    Expression
    Mutation
    M24T (please see depositor comments)
    Promoter
    Available since
    Oct. 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    155015-plasmid-map-sequence-id-314979

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  16. B52 + PARP4 sgSTOP

    Plasmid
    #100711
    Purpose
    B52 plasmid expressing PARP4 sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning)
    Depositor
    Alberto Ciccia
    Article
    Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17
    Insert
    sgSTOP targeting PARP4 (cloned using BbsI)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 promoters
    Available since
    Sept. 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    100711-plasmid-map-sequence-id-193646

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  17. B52 + PIK3R1 sgSTOP

    Plasmid
    #100714
    Purpose
    B52 plasmid expressing PI3KR1 sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning)
    Depositor
    Alberto Ciccia
    Article
    Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17
    Insert
    sgSTOP targeting PIK3R1 (cloned using BbsI)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 promoters
    Available since
    Sept. 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    100714-plasmid-map-sequence-id-193648

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  21. pAK208_2xBRD9BD_Y57A-IFP2.0C

    Plasmid
    #233733
    Purpose
    Expresses BRD9 Bromodomain as tandem double domain Y57A mutant fused to C-terminal part of IFP2.0 as negative control for plasmid 233734
    Depositor
    Albert Jeltsch
    Article
    Köhler et al Genes (Basel). 2025 Apr 10;16(4):444. do
    Insert
    BRD9 (BRD9 Human)
    Use
    Tags
    IFP2.0 C-terminal part
    Expression
    Mammalian
    Mutation
    Bromodomain cloned twice in tandem with Y57A muta…
    Promoter
    CMV
    Available since
    May 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    233733-plasmid-map-sequence-id-472158

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  26. pINQ-ON10

    Plasmid
    #198689
    Purpose
    Periplasmic expression of anti-SARS-Cov-2 Nucleocapsid nanobody ON10 (HA-tagged) in BL21(DE3)
    Depositor
    Gualberto González Sapienza
    Article
    Segovia-de Los Santos et al Front Immunol. 2023 Jul 11;14:1220477. d
    Insert
    anti-SARS-CoV-2 Nucleocapsid nanobody ON10
    Use
    Tags
    6xHis tag, HA tag, OmpA signal peptide, and Ribos…
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    Dec. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    198689-plasmid-map-sequence-id-402802

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  36. pMDC32B-BS-AtMIR390a-B/c

    Plasmid
    #199560
    Purpose
    Plant expression vector (2x35S) for direct cloning of amiRNAs into Arabidopsis thaliana MIR390a precursor including only the basal stem
    Depositor
    Alberto Carbonell
    Article
    Cisneros et al Nucleic Acids Res. 2023 Oct 27;51(19):10
    Insert
    Basal stem of Arabidopsis MIR390a precursor including a chloramphenicol-ccdB cassette flanked by two inverted BsaI sites (MIR390a Mustard Weed)
    Use
    RNAi
    Tags
    Expression
    Mutation
    Promoter
    2x35S
    Available since
    Aug. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    199560-plasmid-map-sequence-id-398170

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  37. PB-Neo-TetO-Cdk12_Nterm_FlagHa

    Plasmid
    #127179
    Purpose
    N-terminal Flag- HA- tandem epitope tagged mouse Cdk12 transgene (NM_001109626.1 isoform) cloned into a dox-inducible piggybac destination vector
    Depositor
    Phil Sharp
    Article
    Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:
    Insert
    N-terminally Flag- HA- Epitope Tagged Mouse Cyclin Dependent Kinase 12 (Cdk12 Mouse)
    Use
    Piggybac transposase vector
    Tags
    Flag- HA- Tandem Epitopes
    Expression
    Mammalian
    Mutation
    Promoter
    TetO Promoter
    Available since
    July 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    127179-plasmid-map-sequence-id-247939

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  38. TTR-bio-His

    Plasmid
    #52091
    Purpose
    Expresses full-length Transthyretin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    TTR (TTR Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    52091-plasmid-map-sequence-id-80367

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  40. pcDNA 3.1-NbON10-NanoLuc

    Plasmid
    #198691
    Purpose
    Expresses anti-SARS-Cov-2 Nucleocapsid nanobody ON10 fused to NanoLuc in HEK293 cell culture
    Depositor
    Gualberto González Sapienza
    Article
    Segovia-de Los Santos et al Front Immunol. 2023 Jul 11;14:1220477. d
    Insert
    anti-SARS-CoV-2 Nucleocapsid nanobody ON10
    Use
    Tags
    6xHis tag, IgK leader sequence, NanoLuc, and twin…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter
    Available since
    Dec. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    198691-plasmid-map-sequence-id-402807

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Showing: 2281 - 2320 of 2376 results