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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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170,334 results

Showing: 11621 - 11640 of 170334 results

  2. TFORF1759

    Plasmid
    #143845
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ALYREF (ALYREF Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    143845-plasmid-map-sequence-id-396272

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  5. pAAV-phSyn-flex-SypEGFP

    Plasmid
    #153203
    Purpose
    Can be used to generate AAV virus that will mark the presynaptic terminal (synaptophysin-fused) EGFP in the presence of Cre in neurons from the synapsin promoter
    Depositor
    Lynette Lim
    Insert
    synaptophysin-EGFP
    Use
    AAV
    Promoter
    rat synapsin
    Available Since
    June 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    153203-plasmid-map-sequence-id-298916

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  7. pENN.AAV.EF1a.eGFP.WPRE.rBG (AAV Retrograde)

    Viral Prep
    #105547-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pENN.AAV.EF1a.eGFP.WPRE.rBG (#105547). In addition to the viral particles, you will also receive purified pENN.AAV.EF1a.eGFP.WPRE.rBG plasmid DNA. EF1a-driven EGFP expression. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    James M. Wilson
    Promoter
    EF1a
    Tags
    EGFP
    Available Since
    Oct. 18, 2021
    Availability
    Academic Institutions and Nonprofits only

  8. pSLPB2B-FKBP12_F36V-hHDAC4-SpdCas9-tagBFP-PGK-Blasticidin

    Plasmid
    #187956
    Purpose
    FKBP12 (F36V mutant) degron-tagged hHDAC4-dCas9 fused with tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.
    Depositor
    Edda Schulz
    Article
    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:
    Insert
    FKBP12_F36V-hHDAC4-SpdCas9-tagBFP
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Available Since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    187956-plasmid-map-sequence-id-373523

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  9. pRK5-myc-RhoA-Q63L

    Plasmid
    #12964
    Depositor
    Gary Bokoch
    Insert
    RhoA constitutively active (RHOA Human)
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Q63L Constitutively active
    Available Since
    Oct. 27, 2006
    Availability
    Academic Institutions and Nonprofits only
    12964-plasmid-map-sequence-id-139200

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  12. pBabe-CD44v6

    Plasmid
    #107970
    Purpose
    expression of CD44 variant
    Depositor
    Georg Weber
    Insert
    mCD44v6 (Cd44 Mouse)
    Use
    Retroviral
    Expression
    Mammalian
    Mutation
    splice variant; see Depositor Comments below
    Available Since
    Jan. 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    107970-plasmid-map-sequence-id-218212

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  18. pMito-MtrxRFP2

    Plasmid
    #172324
    Purpose
    fluorescent biosensor for the redox of mitochondrial specific thioredoxin (Trx2)
    Depositor
    Huiwang Ai
    Article
    Pang et al Biochemistry. 2022 Mar 1;61(5):377-384.
    Insert
    mitochondrial expression of human thioredoxin 2, fused with a redox-sensitive RFP through Gly-Ser-rich linker
    Tags
    His-tag and Mitochondria localization sequence
    Expression
    Mammalian
    Promoter
    CMV, SP6
    Available Since
    Aug. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    172324-plasmid-map-sequence-id-431104

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  19. pEJS654 All-in-One AAV-sgRNA-hNmeCas9

    Plasmid
    #112139
    Purpose
    Delivery of human-codon-optimized Cas9 from Neisseria meningitidis (NmeCas9) and its single-guide RNA in a single AAV vector for in vivo genome editing.
    Depositor
    Erik Sontheimer
    Article
    Ibraheim et al Genome Biol. 2018 Sep 19;19(1):137. doi:
    Inserts
    sgRNA scaffold
    Human-codon-optimized NmeCas9
    Use
    AAV, CRISPR, and Mouse Targeting
    Expression
    Mammalian
    Promoter
    U1a and U6
    Available Since
    Aug. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    112139-plasmid-map-sequence-id-217823

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  20. pAAV-CAG-fDIO-mNeonGreen (AAV1)

    Viral Prep
    #99133-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-CAG-fDIO-mNeonGreen (#99133). In addition to the viral particles, you will also receive purified pAAV-CAG-fDIO-mNeonGreen plasmid DNA. CAG-driven, Flp-dependent mNeonGreen expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Viviana Gradinaru
    Article
    Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.
    Promoter
    CAG
    Tags
    mNeonGreen (Flp-dependent)
    Available Since
    Jan. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
Showing: 11621 - 11640 of 170334 results