We narrowed to 12,986 results for: NUC
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Plasmid#85053PurposeIn vivo visualization of the nucleus (can be used for colocalization studies)DepositorAvailable SinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only
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pSKA413
Plasmid#80381PurposeConstitutive CcaS and CcaR with gfpmut3 under the cpcG2Δ59 promoter.DepositorInsertsccaS (complement)
ccaR (complement)
gfpmut3
UseSynthetic Biology; OptogeneticsTagsFLAG tagExpressionBacterialPromoterccaR and cpcG2Δ59Available SinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SEMPER_ACC-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
Plasmid#221075PurposeSEMPER plasmid for expression of msfGFP[r5M] and mEBFP2 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publicationDepositorInsertACC-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
ExpressionMammalianPromoterCMVAvailable SinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pICH47811-pAtAct2::Cas9-nls::tSlATPase
Plasmid#160221PurposeCas9-nuclear localization, pACT2 promoter, SlATPase terminator transcriptional unit, inserted at the MoClo toolkit level 1 acceptor position 2 reverseDepositorInsertpAtACT2::Cas9-nls::tSlATPase
UseSynthetic Biology; Moclo toolkit level 1 acceptor…ExpressionPlantAvailable SinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENTR_L1-miniTurbo-YFP-NLS-STOP-L2
Plasmid#127362Purposegateway entry vector for expressing nuclear miniTurbo-YFP under a promoter of choiceDepositorInsertminiTurbo (BirA mutant)
UseGateway entry vectorTagsGS linker, NLS, V5, and YFPMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …Promoterno promoterAvailable SinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET30a-SARS-CoV2-NP
Plasmid#165090Purposeprotein expression in E. coliDepositorInsertSevere acute respiratory syndrome coronavirus 2 nucleocapsid protein N-terminal (N SARS-CoV2)
ExpressionBacterialAvailable SinceFeb. 10, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
SiC-V2-Cas9G7
Plasmid#133043PurposeDoxycycline-inducible SiC-V2 vector with Cas9G7 (sgRNA 7 targeting SpCas9 gene). In cells coexpressing Cas9 nuclease, this construct causes self-inactivation/editing of SpCas9 gene upon Dox addition.DepositorInsertTet repressor
UseLentiviralTagsCeruleanAvailable SinceNov. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-dCas9/pMXs-puro
Plasmid#51240PurposeExpresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.DepositorInsert3xFLAG-dCas9
UseCRISPR and RetroviralTags3xFLAG tag and NLS (nuclear localization signal)ExpressionMammalianMutationhuman codon-optimized, D10A + H840APromoterLTRAvailable SinceAug. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-HyPer-DAAO-NES
Plasmid#119164PurposeFusion of hydrogen peroxide fluorescent biosensor HyPer and D-amino acid oxidase; excluded from nucleus. Allows H2O2 production/detection by adding D-amino acids. AAV expression vector; CMV promoter.DepositorInsertHyPer-DAAO-NES
UseAAVTagsNESExpressionMammalianPromoterCMVAvailable SinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pST1374-N-NLS-flag-linker-Cas9-D10A
Plasmid#51130PurposeCas9 nickase optimized for nuclear import.DepositorInsertCas9 nickase
UseCRISPRTagsFlag and NLSExpressionMammalianMutationD10A (nickase mutant)PromoterCMVAvailable SinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLentiRNACRISPR_003 - hU6-DR_BsmBI-EFS-PspCas13b-NLS-2A-Puro-WPRE
Plasmid#138145PurposeExpresses PspCas13b in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with PspCas13b. Contains a 3' direct repeat. Clone using BsmBI.DepositorInsertPspCas13b
UseLentiviralTagsNLSAvailable SinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV hUbC-dSaCas9-KRAB-T2A-PuroR
Plasmid#106249PurposeLentiviral vector with puro selection for expression of S. aureus dCas9-KRABDepositorInsertdead S. aureus Cas9 KRAB T2A PuroR
UseCRISPR and LentiviralTagsHA-NLS and NLS-KRABExpressionMammalianMutationD10A and N580APromoterhUbCAvailable SinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SEMPER_GGG-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
Plasmid#221076PurposeSEMPER plasmid for expression of msfGFP[r5M] and mEBFP2 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publicationDepositorInsertGGG-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
ExpressionMammalianPromoterCMVAvailable SinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SEMPER_CCC-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
Plasmid#221077PurposeSEMPER plasmid for expression of msfGFP[r5M] and mEBFP2 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publicationDepositorInsertCCC-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
ExpressionMammalianPromoterCMVAvailable SinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SEMPER_TTT-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
Plasmid#221079PurposeSEMPER plasmid for expression of msfGFP[r5M] and mEBFP2 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publicationDepositorInsertTTT-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
ExpressionMammalianPromoterCMVAvailable SinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR_L1-Turbo-YFP-NLS-STOP-L2
Plasmid#127361Purposegateway entry vector for expressing nuclear TurboID-YFP under a promoter of choiceDepositorInsertTurboID (BirA mutant)
UseGateway entry vectorTagsGS linker, NLS, V5, and YFPMutationQ65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…Promoterno promoterAvailable SinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGH00.0126
Plasmid#64257PurposeBinary T-DNA vector expressing EGFP and neomycin phosphotransferase II (NPTII); for Agrobacterium tumefaciens-mediated transformationDepositorInsertsEGFP
neomycin phosphotransferase II
UseBinary agrobacterium vector for plant transformat…ExpressionPlantPromoterE12-omegaAvailable SinceJune 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV.U6gT28.4.hSyn.H2B.RFP
Plasmid#170387PurposePlasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 4. Expressing nuclear RFP.DepositorInsertH2B RFP
UseAAVPromoterhuman SynapsinAvailable SinceJan. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-NLS-CBRed-d2-hygro
Plasmid#139196Purposenon-ERK dependent control plasmid for pMSCV-NLS-CBGreen-FIRE-puroDepositorInsertCBRed
UseLuciferase and RetroviralTagsd2 PEST domain and nuclear localization signal (N…ExpressionMammalianAvailable SinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR SFFVp BFP-Erk2
Plasmid#50848PurposeFluorescent reporter for Erk nuclear translocationDepositorAvailable SinceJan. 9, 2015AvailabilityAcademic Institutions and Nonprofits only