166,551 results

1. pGL3 Promoter Vector

   Plasmid

   #212939

   Purpose

   Control vector with constitutive firefly luciferase expression

   Depositor

   Debrya Groskreutz

   Insert

   luc+
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   WT

   Promoter

   SV40

   Available Since

   Jan. 16, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pEF-Bos TRIF Flag

   Plasmid

   #41550

   Depositor

   Kate Fitzgerald, Tom Maniatis

   Article

   Fitzgerald et al Nat Immunol. 2003 May;4(5):491-6.

   Insert

   Trif (TICAM1 Human)
   

   Use

   Tags

   6xHis and Flag

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Feb. 26, 2013

   Availability

   Academic Institutions and Nonprofits only

3. AFDN_PDZ_1

   Plasmid

   #103938

   Purpose

   Protein expression and purification of TRITHORAX PDZ domain

   Depositor

   Sachdev Sidhu

   Article

   Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

   Insert

   AFDN (AFDN Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   contains AA983-1110 of AFDN

   Promoter

   tac

   Available Since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAuroraA-GFP-AURKA

   Plasmid

   #99877

   Purpose

   Expression of GFP-AURKA in mammalian cells

   Depositor

   Marc Tramier

   Article

   Bertolin et al Nat Commun. 2016 Sep 14;7:12674. doi: 10

   Insert

   AURKA (AURKA Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   AURKA minimal

   Available Since

   Sept. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. LifeAct-GFP-cytoB5RR

   Plasmid

   #182577

   Purpose

   Expresses LifeAct-GFP targeted to the ER via the CytB5RR tail anchor sequence

   Depositor

   Uri Manor

   Article

   Schiavon et al Nat Methods. 2020 Sep;17(9):917-921. doi

   Insert

   LifeAct-GFP-cytoB5RR
   

   Use

   Tags

   LifeAct and cytoB5RR

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV.hSynap.iAChSnFR

   Plasmid

   #137950

   Purpose

   Expresses iAChSnFR under hSynapsin promoter

   Depositor

   Loren Looger

   Article

   Borden et al bioRxiv 2020

   Has Service

   AAV1

   Insert

   iAChSnFR
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSynanpsin

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. P3-Lenti-dCas9-Tet1-GFP

   Plasmid

   #190729

   Purpose

   Lentiviral construct to express spdCas9-Tet1-GFP.

   Depositor

   Maja Jagodic

   Article

   Pahlevan Kakhki et al Nat Commun. 2024 Jul 30;15(1):6419. doi:

   Insert

   dCas9-tet1
   

   Use

   CRISPR

   Tags

   SV40 NLS and T2A-EGFP

   Expression

   Mammalian

   Mutation

   Changed Pro434 codon from CCG to CCC and Gly468 f…

   Promoter

   UbC

   Available Since

   July 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. EGFP-IC2-FL

   Plasmid

   #51409

   Purpose

   expression of EGFP-tagged full-length dynein intermediate chain IC2C in mammalian cells

   Depositor

   Trina Schroer

   Article

   King et al Mol Biol Cell. 2003 Dec;14(12):5089-97.

   Insert

   dynein intermediate chain IC2C (Dync1i2 Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pB-Rex

   Plasmid

   #122134

   Purpose

   B. subtilis Rex sensor plasmid with gentamicin resistance and pBBR1 origin

   Depositor

   Srivatsan Raman

   Article

   Liu et al ACS Synth Biol. 2019 Feb 15;8(2):264-273

   Insert

   B. subtilis Rex
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   apFAB338

   Available Since

   Feb. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. scFLARE2

    Plasmid

    #158700

    Purpose

    Neuronal expression of scFLARE2 component

    Depositor

    Alice Ting

    Article

    Sanchez et al Proc Natl Acad Sci U S A. 2020 Dec 15. p

    Insert

    scFLARE2
    

    Use

    AAV

    Tags

    HA, Flag, VP16

    Expression

    Mammalian

    Mutation

    Truncated form of TEV protease (220-242 amino aci…

    Promoter

    Syn

    Available Since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-Roxed-Cre

    Plasmid

    #51273

    Purpose

    Component of the Co-Driver binary site-specific recombinase system, generates functional rox60-71Cre upon removal of an internal STOP cassette by Dre

    Depositor

    Pawel Pelczar

    Article

    Hermann et al Nucleic Acids Res. 2014 Jan 9.

    Insert

    Roxed-Cre
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    June 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-EF1a-double floxed-Gad67-WPRE-HGHpA

    Plasmid

    #184632

    Purpose

    Expresses Gad67, driven by the Ef1a promoter, in a Cre-dependent fashion

    Depositor

    Nicolas Tritsch

    Article

    Melani et al Cell Rep. 2022 Apr 19;39(3):110716. doi:

    Insert

    Gad67 (Gad1 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. iTol2Amp-γ-crystallin:RFP

    Plasmid

    #108455

    Purpose

    This plamid can be used to recombine the iTol2-Amp-γ-crystallin:RFP into a BAC.

    Depositor

    Nadia Mercader Huber

    Article

    Sanchez-Iranzo et al Nat Commun. 2018 Jan 30;9(1):428. doi: 1

    Insert

    Tol2-Amp-y-cryst-mCherry
    

    Use

    Gateway

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMAL-SH3(2)-5R

    Plasmid

    #112089

    Purpose

    Bacterial expression plasmid containing His and MBP tags for 5 repeats of the second SH3 domain from human NCK1.

    Depositor

    Michael Rosen

    Article

    Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

    Insert

    SH3(2)-5R (NCK1 Human)
    

    Use

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    construct contains only repeats of the second SH3…

    Promoter

    Lac

    Available Since

    Feb. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28 MBP NAAEF-AMPKa1 (13-476,529-550)-b2(76-272)-g1(24-327)

    Plasmid

    #177850

    Purpose

    Bacterial Expression of AMPK

    Depositor

    Karsten Melcher

    Article

    Yan et al Science. 2021 Jul 23;373(6553):413-419.

    Insert

    AMPK (a1b2g1)
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMal-Abl-PRM 5R

    Plasmid

    #112088

    Purpose

    Bacterial expression plasmid containing His and MBP tags for 5 PRM motif repeats of Human Abl.

    Depositor

    Michael Rosen

    Article

    Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

    Insert

    PRM-5R (ABL1 Human)
    

    Use

    Tags

    His-6 and MBP

    Expression

    Bacterial

    Mutation

    construct contains only the PRM motif of Abl1

    Promoter

    Lac

    Available Since

    Feb. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pINDUCER20_mGFP

    Plasmid

    #190027

    Purpose

    Doxycycline-inducible lentiviral vector for expression of ORF with C-terminal mEGFP

    Depositor

    Andrew Tutt

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-EF1a-double floxed-Gad65-WPRE-HGHpA

    Plasmid

    #184631

    Purpose

    Expresses Gad65, driven by the Ef1a promoter, in a Cre-dependent fashion

    Depositor

    Nicolas Tritsch

    Article

    Melani et al Cell Rep. 2022 Apr 19;39(3):110716. doi:

    Insert

    Gad65 (Gad2 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. (SUMO)10

    Plasmid

    #126948

    Purpose

    Bacterial expression plasmid for (SUMO)10 client to assess SUMO-SIM scaffolds.

    Depositor

    Michael Rosen

    Article

    Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

    Insert

    10 repeats of human SUMO3 each separated by (GGS)4 (SUMO3 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    unknown

    Available Since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTol1-UAS:GtACR1-tdTomato

    Plasmid

    #124235

    Purpose

    Zebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin GtACR1 fused to the red fluorescent protein tdTomato

    Depositor

    Isaac Bianco

    Article

    Antinucci et al Elife. 2020 Mar 27;9. pii: 54937. doi: 1

    Insert

    GtACR1-tdTomato
    

    Use

    Zebrafish expression

    Tags

    tdTomato

    Expression

    Mutation

    Promoter

    14xUAS-E1b

    Available Since

    Jan. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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