We narrowed to 24,908 results for: Spr

1. pL-CRISPR.EFS.PAC

   Plasmid

   #57828

   Purpose

   Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA, Puromycin resistance, EFS Promoter driven

   Depositor

   Benjamin Ebert

   Article

   Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

   Inserts

   SpCas9
   Sp sgRNA scaffold
   EFS (EEF1A1 Human)
   P2A-PAC
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   Available since

   Aug. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. lentiCRISPRv2_PROX1_1+3

   Plasmid

   #121176

   Purpose

   Lentiviral construct for the expression of Cas9 protein and puromycin resistance from EFS promoter and two guide RNAs targeting for deletion PROX1 start codon sequence.

   Depositor

   Timo Otonkoski

   Insert

   Cas9-P2A-puro and U6 promoters driven expression of gRNAs
   

   Use

   CRISPR and Lentiviral

   Tags

   P2A-puro

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLentiCRISPRv1_SDHA

   Plasmid

   #177980

   Purpose

   lentiviral vector expressing Cas9 and a sgRNA targeting SDHA

   Depositor

   David Sabatini, Jessica Spinelli

   Article

   Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

   Insert

   sgRNA targeting SDHA (SDHA Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   U6

   Available since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. SpRY

   Plasmid

   #198544

   Purpose

   Expresses human codon-optimized SpRY and blasticidin resistance: EFS promoter-SpRY-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

   Insert

   SpRY
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLentiCRISPRv1_UQCRC2

   Plasmid

   #177982

   Purpose

   lentiviral vector expressing Cas9 and a sgRNA targeting UQCRC2

   Depositor

   David Sabatini, Jessica Spinelli

   Article

   Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

   Insert

   sgRNA targeting UQCRC2 (UQCRC2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   U6

   Available since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLSPR24

   Plasmid

   #217309

   Purpose

   Promoterless (SwaI Cutsite) mRuby3:amdS fusion reporter

   Depositor

   James Fraser

   Article

   Phillips-Rose et al J Fungi (Basel). 2024 Aug 12;10(8):567.

   Insert

   Acetamidase
   

   Use

   Tags

   mRuby3

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLSPR25

   Plasmid

   #217310

   Purpose

   Promoterless (SwaI Cutsite) mNeonGreen:amdS fusion reporter

   Depositor

   James Fraser

   Article

   Phillips-Rose et al J Fungi (Basel). 2024 Aug 12;10(8):567.

   Insert

   Acetamidase
   

   Use

   Tags

   mNeonGreen

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLSPR30

   Plasmid

   #217315

   Purpose

   GAL7(p)-mRuby3:amdS fusion reporter

   Depositor

   James Fraser

   Article

   Phillips-Rose et al J Fungi (Basel). 2024 Aug 12;10(8):567.

   Insert

   Acetamidase
   

   Use

   Tags

   mRuby3

   Expression

   Yeast

   Mutation

   Promoter

   GAL7

   Available since

   Sept. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLSPR31

   Plasmid

   #217316

   Purpose

   TEF1(p)-mRuby3:amdS fusion reporter

   Depositor

   James Fraser

   Article

   Phillips-Rose et al J Fungi (Basel). 2024 Aug 12;10(8):567.

   Insert

   Acetamidase
   

   Use

   Tags

   mRuby3

   Expression

   Yeast

   Mutation

   Promoter

   TEF1

   Available since

   Sept. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLSPR32

    Plasmid

    #217317

    Purpose

    GAL7(p)-mNeonGreen:amdS fusion reporter

    Depositor

    James Fraser

    Article

    Phillips-Rose et al J Fungi (Basel). 2024 Aug 12;10(8):567.

    Insert

    Acetamidase
    

    Use

    Tags

    mNeonGreen

    Expression

    Yeast

    Mutation

    Promoter

    GAL7

    Available since

    Sept. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLSPR33

    Plasmid

    #217318

    Purpose

    TEF1(p)-mNeonGreen:amdS fusion reporter

    Depositor

    James Fraser

    Article

    Phillips-Rose et al J Fungi (Basel). 2024 Aug 12;10(8):567.

    Insert

    Acetamidase
    

    Use

    Tags

    mNeonGreen

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    Sept. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCRISPRai

    Plasmid

    #189938

    Purpose

    Expresses CRISPRai cassette under EF1a promoter

    Depositor

    Yu-Chen Hu

    Article

    Truong et al Nucleic Acids Res. 2019 Jul 26;47(13):e7

    Insert

    CRISPRai cassette
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV enhancer-rat EF1 alpha

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pL-CRISPR.SFFV.PAC

    Plasmid

    #57829

    Purpose

    Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA, Puromycin resistance, SFFV Promoter driven

    Depositor

    Benjamin Ebert

    Article

    Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

    Inserts

    SpCas9
    Sp sgRNA scaffold
    SFFV
    P2A-PAC
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    Available since

    Aug. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCRISPR_eGFP_497

    Plasmid

    #111824

    Purpose

    Knockout eGFP

    Depositor

    Steve Shih

    Article

    Sinha et al Lab Chip. 2018 Jul 24;18(15):2300-2312.

    Insert

    gRNA targeting eGFP 477-497
    

    Use

    CRISPR

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLentiCRISPRv4

    Plasmid

    #221432

    Purpose

    Lentiviral CRISPR/Cas9 vector for co-expression of sgRNAs and Cas9 protein.

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCRISPReporter

    Plasmid

    #65007

    Purpose

    E. coli reporter vector with MCS for inserting bacterial promoter region through 5' end of gene of interest, upstream and in-frame with reporter cloning site

    Depositor

    Mattheos Koffas

    Article

    Cress et al ACS Synth Biol. 2015 Mar 30.

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    none

    Available since

    May 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCRISPR_eGFP_191

    Plasmid

    #111821

    Purpose

    Knockout eGFP

    Depositor

    Steve Shih

    Article

    Sinha et al Lab Chip. 2018 Jul 24;18(15):2300-2312.

    Insert

    gRNA targeting eGFP 171-191
    

    Use

    CRISPR

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. Lenti_CRISPRi_sgNeg1

    Plasmid

    #166998

    Purpose

    Lentiviral expression of negative control sgRNA for CRISPRi knockdown

    Depositor

    Gian-Paolo Dotto

    Article

    Ma et al J Exp Med. 2021 Feb 1;218(2). pii: 21150

    Insert

    Negative control sgRNA 1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    April 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCRISPRi-D2

    Plasmid

    #182927

    Purpose

    inducible CRISPRi plasmid with gRNA targeting to psbD gene in Synechocystis 6803

    Depositor

    Himadri Pakrasi

    Article

    Liu et al ACS Synth Biol. 2020 Jun 19;9(6):1441-14

    Insert

    ddcpf1
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. CRISPR_TRAC

    Plasmid

    #164993

    Purpose

    Expression of gRNA targeting TCRalpha constant locus

    Depositor

    Matthew Meyerson

    Article

    Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

    Insert

    gRNA against TRAC
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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