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We narrowed to 7,508 results for: ef1a

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Showing: 1801 - 1830 of 7508 results

  1. pLVX-EF1alpha-SARS-CoV-2-nsp5-C145A-2xStrep-IRES-Puro

    Plasmid
    #141371
    Purpose
    Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus
    Depositor
    Nevan Krogan
    Article
    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:
    Insert
    SARS-CoV-2-nsp5-C145A (ORF1ab SARS-CoV-2)
    Use
    Lentiviral
    Tags
    2xStrep
    Expression
    Mammalian
    Mutation
    C145A, human codon optimized
    Promoter
    EF1alpha
    Available Since
    April 6, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    141371-plasmid-map-sequence-id-288079

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  3. Lenti-N1303K-tevopreQ1-epegRNA+13C>G_EF1a-puroR (PBS 14 - RTT 18)

    Plasmid
    #207356
    Purpose
    Lentiviral transfer plasmid encoding hU6-driven expression of a N1303K-CFTR correcting prime editing guide (epegRNA) and EF1a-driven puromycin resistance gene.
    Depositor
    Marianne Carlon
    Article
    Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d
    Insert
    N1303K-CFTR + 13 C>G tevopreq1-prime editing guide RNA (epegRNA)
    Use
    Lentiviral
    Available Since
    April 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    207356-plasmid-map-sequence-id-408417

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  10. Lenti-L227R-tevopreQ1-epegRNA+13C>A_EF1a-puroR (PBS 10 - RTT 19)

    Plasmid
    #207357
    Purpose
    Lentiviral transfer plasmid encoding hU6-driven expression of a L227R-CFTR correcting prime editing guide (epegRNA) and EF1a-driven puromycin resistance gene.
    Depositor
    Marianne Carlon
    Article
    Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d
    Insert
    L227R-CFTR + 13 C>A tevopreq1-prime editing guide RNA (epegRNA)
    Use
    Lentiviral
    Available Since
    April 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    207357-plasmid-map-sequence-id-408418

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  11. pAAV-EF1a>WAChR_1s-G-mScarlet-Soma-E; WPRE3-BGH polyA; AmpR

    Plasmid
    #247725
    Purpose
    Constitutive expression of WAChR-1s opsin fusion protein: {WAChR-1s}-{Kir2.1 Golgi tag}-{mScarlet}-{Kv2.1 soma tag}-{Kir2.1 ER tag}
    Depositor
    Alexander Naka
    Article
    Tose et al bioRxiv 2025.09.12.675947; doi: https://
    Insert
    WAChR_1s-G-mScarlet-Soma-E
    Use
    AAV
    Tags
    mScarlet
    Expression
    Mammalian
    Mutation
    F211W/F240A
    Promoter
    EF1a
    Available Since
    Dec. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    247725-plasmid-map-sequence-id-495874

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  12. pAAV-EF1a>WAChR_1f-G-mScarlet3-Soma-E; WPRE3-BGH polyA; AmpR

    Plasmid
    #247723
    Purpose
    Constitutive expression of WAChR-1f opsin fusion protein: {WAChR-1f}-{Kir2.1 Golgi tag}-{mScarlet3}-{Kv2.1 soma tag}-{Kir2.1 ER tag}
    Depositor
    Alexander Naka
    Article
    Tose et al bioRxiv 2025.09.12.675947; doi: https://
    Insert
    WAChR_1f-G-mScarlet3-Soma-E
    Use
    AAV
    Tags
    mScarlet3
    Expression
    Mammalian
    Mutation
    L130F/F240A
    Promoter
    EF1a
    Available Since
    Dec. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    247723-plasmid-map-sequence-id-495872

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  13. pAAV-EF1a>WAChR_1m-G-mScarlet-Soma-E; WPRE3-BGH polyA; AmpR

    Plasmid
    #247724
    Purpose
    Constitutive expression of WAChR-1m opsin fusion protein: {WAChR-1m}-{Kir2.1 Golgi tag}-{mScarlet}-{Kv2.1 soma tag}-{Kir2.1 ER tag}
    Depositor
    Alexander Naka
    Article
    Tose et al bioRxiv 2025.09.12.675947; doi: https://
    Insert
    WAChR_1m-G-mScarlet-Soma-E
    Use
    AAV
    Tags
    mScarlet
    Expression
    Mammalian
    Mutation
    S122L/F240A
    Promoter
    EF1a
    Available Since
    Dec. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    247724-plasmid-map-sequence-id-495858

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  15. pAAV-Ef1a-Con/Fon-taCasp3-T2A-TEVp-WPRE3-BGHpA

    Plasmid
    #242456
    Purpose
    AAV plasmid for expressing taCaspase-3 and T2A linked TEVp for caspase mediated cell ablation, and an intersectional strategy for Cre-off and Flp-on expression. See comments for more detail.
    Depositor
    Andrew Steele
    Insert
    Caspase 3 (CASP3 Human, Synthetic)
    Expression
    Mammalian
    Promoter
    Ef1a
    Available Since
    Dec. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    242456-plasmid-map-sequence-id-492090

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  21. pLV-Hygro-EF1a-XRCC1-EGFP-T2A-Myc-POLB(PAMmut)

    Plasmid
    #176139
    Purpose
    EGFP fused to the C-terminus of XRCC1, linked by T2A to N-terminus Myc-tagged POLB with a mutation in the PAM site used by POLBKO gRNA1 & a hygromycin resistance cassette
    Depositor
    Robert Sobol
    Article
    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:
    Insert
    XRCC1-T2A-PolB (XRCC1, POLB Human)
    Use
    Lentiviral
    Tags
    EGFP and MYC
    Expression
    Mammalian
    Promoter
    EF1A
    Available Since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    176139-plasmid-map-sequence-id-353012

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  23. AAV-EF1a-DIO-oChIEF(E163A/T199C)-P2A-dTomato-WPRE-BGHpA

    Plasmid
    #51094
    Purpose
    Cre dependent expression of oChIEF kinetic variant E163A/T199C with physically uncoupled dTomato fluorophore
    Depositor
    Jonathan Ting
    Insert
    oChIEF(E163A/T199C)
    Use
    AAV
    Tags
    P2A-dTomato
    Expression
    Mammalian
    Mutation
    E163A/T199C
    Promoter
    EF1a
    Available Since
    May 13, 2014
    Availability
    Academic Institutions and Nonprofits only
    51094-plasmid-map-sequence-id-79315

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  25. pPB-Ins-TRE3Gp-KRAB-dCas9-ecDHFR-IRES-GFP-EF1Ap-Puro

    Plasmid
    #183410
    Purpose
    piggyBAC-based doxycycline- and trimethoprim-inducible KRAB-dCas9 CRISPR interference plasmid for enhancer and promoter repression in mammalian cells
    Depositor
    Azim Surani
    Article
    Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d
    Insert
    KRAB-dCas9-ecDHFR
    Use
    CRISPR and Synthetic Biology; Piggybac
    Expression
    Mammalian
    Promoter
    TRE3G
    Available Since
    May 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    183410-plasmid-map-sequence-id-361256

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  27. CLYBL-(Ef1a-SBP-LNGFR-T2A-mApple)-(CAG-rtTA)-(TRE-hNIL))

    Plasmid
    #105842
    Purpose
    Donor construct for introduction of hNIL factors and SBP-LNGFR for streptavidin bead enrichment
    Depositor
    Michael Ward
    Article
    Fernandopulle et al Curr Protoc Cell Biol. 2018 Jun;79(1):e5
    Inserts
    Neurogenin 2 (NEUROG2 Human)
    ISL LIM Homeobox 1 (ISL1 Human)
    LIM Homeobox 3 (LHX3 Human)
    Optimized mApple
    rtTA3G
    SBP Delta-LNGFR
    Use
    CRISPR and TALEN
    Promoter
    CAG, EF-1alpha, and TRE3G
    Available Since
    April 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    105842-plasmid-map-sequence-id-393690

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  30. pTBL1269 pLenti-CHYRON17-pEF1a(short)-Luc2-P2A-tdTomato-T2A-TdT

    Plasmid
    #163643
    Purpose
    Makes a lentivirus that expresses hgRNA with 17nt spacer and Luc2, tdTomato, and human TdT.
    Depositor
    Chang Liu
    Article
    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d
    Inserts
    pU6-CHYRON17 hgRNA
    Luc2-P2A-tdTomato-T2A-human TdT (DNTT Human)
    Use
    Lentiviral
    Tags
    Luc2-P2A-tdTomato-T2A
    Mutation
    The SpCas9 sgRNA constant region is mutated to ma…
    Promoter
    EFS and pU6
    Available Since
    Feb. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    163643-plasmid-map-sequence-id-325021

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Showing: 1801 - 1830 of 7508 results