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We narrowed to 6,280 results for: PEP

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Showing: 3051 - 3100 of 6280 results

  2. pET28a-Fgß-ddFLN4-HIS-ybbr

    Plasmid
    #101239
    Purpose
    wildtype Fgß peptide with ddFLN4 fingerprint domain with C-terminal ybbr tag for covalent immobilization - resulting in high forces
    Depositor
    Hermann Gaub
    Article
    Milles et al Science. 2018 Mar 30;359(6383):1527-1533
    Insert
    Fgß peptide, ddFLN4 fingerprint (FGB D. discoideum, Human)
    Tags
    HIS and ybbr
    Expression
    Bacterial
    Mutation
    C18S in ddFLN4
    Promoter
    T7
    Available Since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    101239-plasmid-map-sequence-id-199088

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  7. RXFP1-DuET

    Plasmid
    #213373
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    RXFP1 (RXFP1 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213373-plasmid-map-sequence-id-430890

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  9. yPet_cp229-Binder

    Plasmid
    #179137
    Purpose
    YPet circularly permuted at a.a. 229; Binder: SspB that carries two point mutations reducing homodimerization(Y11K/A15E, residue numbering from pdb: 1ou9).
    Depositor
    Klaus Hahn
    Article
    Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23.
    Insert
    SspB
    Tags
    yPet_cp229
    Expression
    Mammalian
    Mutation
    Y11K/A15E, residue numbering from pdb: 1ou9
    Available Since
    Jan. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    179137-plasmid-map-sequence-id-353407

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  10. FPR3-DuET

    Plasmid
    #213241
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    FPR3 (FPR3 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213241-plasmid-map-sequence-id-430898

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  11. gNS2B47NS3 L30SF31S

    Plasmid
    #155316
    Purpose
    DENV4 47 peptides from NS2B cofactor linked to NS3 full length by GGGGSGGGG linker L30SF31S mutant
    Depositor
    Dahai Luo
    Article
    Phoo et al Antiviral Res. 2020 Oct;182:104900. doi:
    Insert
    DENV4 NS3 full length protein linked with 47 peptides from NS2B cofactor
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    L30 to S, F31 to S
    Promoter
    M13
    Available Since
    Sept. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    155316-plasmid-map-sequence-id-311228

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  13. pAAV-DYRK2-2A-mCherry

    Plasmid
    #118285
    Purpose
    Expresses mouse DYRK2 tagged with 2A peptide at it's C-terminus and mCherry in mammalian cells.
    Depositor
    Vance Lemmon
    Article
    Slepak et al Cytoskeleton (Hoboken). 2012 Jul;69(7):5
    Insert
    dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 2 (Dyrk2 Mouse)
    Use
    AAV
    Tags
    2A peptide and mCherry
    Expression
    Mammalian
    Promoter
    CMV with beta global intron
    Available Since
    Nov. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    118285-plasmid-map-sequence-id-229470

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  17. pAAV-DYRK3-2A-mCherry

    Plasmid
    #118287
    Purpose
    Expresses mouse DYRK3 tagged with 2A peptide at it's C-terminus and mCherry in mammalian cells.
    Depositor
    Vance Lemmon
    Article
    Slepak et al Cytoskeleton (Hoboken). 2012 Jul;69(7):5
    Insert
    dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 3 (Dyrk3 Mouse)
    Use
    AAV
    Tags
    2A peptide and mCherry
    Expression
    Mammalian
    Promoter
    CMV with beta global intron
    Available Since
    Nov. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    118287-plasmid-map-sequence-id-229469

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  21. TFORF2156

    Plasmid
    #142179
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    RHOXF2 (RHOXF2 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    142179-plasmid-map-sequence-id-397685

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  27. VIPR1

    Plasmid
    #51865
    Purpose
    Expresses full-length Vasoactive intestinal polypeptide receptor 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    VIPR1 (VIPR1 Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    51865-plasmid-map-sequence-id-79760

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  31. pMT-ubb-cytoBirA-2a-mCherry

    Plasmid
    #79885
    Purpose
    Ubiquitin promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein; flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Article
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
    Tags
    3x HA
    Expression
    Bacterial
    Promoter
    Ubiquitin
    Available Since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    79885-plasmid-map-sequence-id-150051

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  32. SCUBE1-bio-His

    Plasmid
    #53415
    Purpose
    Expresses full-length Signal peptide, CUB and EGF-like domain-containing protein 1 ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    SCUBE1 (SCUBE1 Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    53415-plasmid-map-sequence-id-83199

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  36. pMT-MCS-cytoBirA-2A-mCherry_Ras

    Plasmid
    #80058
    Purpose
    MCS for cloning promoter to drive HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Article
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
    Tags
    3x HA
    Expression
    Bacterial
    Available Since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    80058-plasmid-map-sequence-id-152592

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  39. pET28a-ybbr-HIS-ddFLN4-K10

    Plasmid
    #101718
    Purpose
    K10 peptide with ddFLN4 fingerprint domain and N-terminal ybbr tag for covalent immobilization - resulting in high forces
    Depositor
    Hermann Gaub
    Article
    Milles et al Science. 2018 Mar 30;359(6383):1527-1533
    Insert
    ddFLN4 fingerprint, K10 peptide (KRT10 Dictyostelium discoideum, Human)
    Tags
    HIS and ybbr
    Expression
    Bacterial
    Mutation
    C18S in ddFLN4
    Promoter
    T7
    Available Since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    101718-plasmid-map-sequence-id-196776

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  43. pMT-flk1-cytoBirA-2A-mCherry_Ras

    Plasmid
    #80056
    Purpose
    Flk1/Kdrl promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Article
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
    Tags
    3x HA
    Expression
    Bacterial
    Promoter
    flk1
    Available Since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    80056-plasmid-map-sequence-id-151372

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  44. pET28a_6HT-TBP6.7(R49A)

    Plasmid
    #112726
    Purpose
    Arg49 of TBP6.7 and other TBPs is the only arginine that was lab-evolved from wild-type U1A to play a significant role in TAR RNA recognition. Mutation results in ~230-fold reduction in Kd.
    Depositor
    Joseph Wedekind
    Article
    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64
    Insert
    6His-TEV-TBP6.7(R49A)
    Tags
    6His-TEV
    Expression
    Bacterial
    Mutation
    R49A
    Promoter
    T7
    Available Since
    Aug. 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    112726-plasmid-map-sequence-id-216329

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  45. pET28a-ybbr-HIS-ddFLN4-Fgß

    Plasmid
    #101719
    Purpose
    wildtype Fgß peptide with ddFLN4 fingerprint domain with N-terminal ybbr tag for covalent immobilization - resulting in weak forces
    Depositor
    Hermann Gaub
    Article
    Milles et al Science. 2018 Mar 30;359(6383):1527-1533
    Insert
    ddFLN4 fingerprint, Fgß peptide (FGB Dictyostelium discoideum, Human)
    Tags
    HIS and ybbr
    Expression
    Bacterial
    Mutation
    C18S in ddFLN4
    Promoter
    T7
    Available Since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    101719-plasmid-map-sequence-id-199092

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  46. pAAV-DYRK2-2A-GFP

    Plasmid
    #118284
    Purpose
    Expresses mouse DYRK2 tagged with 2A peptide at it's C-terminus and GFP in mammalian cells.
    Depositor
    Vance Lemmon
    Article
    Slepak et al Cytoskeleton (Hoboken). 2012 Jul;69(7):5
    Insert
    dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 2 (Dyrk2 Mouse)
    Use
    AAV
    Tags
    2A peptide and GFP
    Expression
    Mammalian
    Promoter
    CMV with beta global intron
    Available Since
    Nov. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    118284-plasmid-map-sequence-id-229473

    Enlarge

  50. pAAV-DYRK4-2A-GFP

    Plasmid
    #118288
    Purpose
    Expresses mouse DYRK4 tagged with 2A peptide at it's C-terminus and GFP in mammalian cells.
    Depositor
    Vance Lemmon
    Article
    Slepak et al Cytoskeleton (Hoboken). 2012 Jul;69(7):5
    Insert
    dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 4 (Dyrk4 Mouse)
    Use
    AAV
    Tags
    2A peptide and EGFP
    Expression
    Mammalian
    Promoter
    CMV with beta global intron
    Available Since
    Nov. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    118288-plasmid-map-sequence-id-229495

    Enlarge

Showing: 3051 - 3100 of 6280 results