We narrowed to 3,403 results for: aaas

1. pSV_229

   Plasmid

   #122934

   Purpose

   Expression of the marked mRNA under the control of substitutional promoters.

   Depositor

   Attila Becskei

   Article

   Baudrimont et al Sci Adv. 2017 Jul 12;3(7):e1700006. doi:

   Insert

   YBR026C (Marked)
   

   Expression

   Yeast

   Mutation

   YBR026C: bp 52-63 AAGCACTTCAAA to AAACATTTTAAG

   Promoter

   [tetO2]4inPgal1

   Available Since

   April 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCMV-EPN-01(ΔL1+L2)

   Plasmid

   #80051

   Purpose

   Expression of EPN-01 mutated in both L domains

   Depositor

   Wesley Sundquist

   Article

   Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

   Insert

   EPN-01(deltaPTAP/deltaYP)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   PTAP10AAAA, YP37AA

   Promoter

   CMV

   Available Since

   Jan. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCJ1021

   Plasmid

   #228761

   Purpose

   Plasmid expressing Cas9 and 2 gRNAs for mouse Ift88. Use for disruption of mouse Ift88 in cultured cells.

   Depositor

   Bradley Yoder

   Insert

   Ift88 gRNAs (Ift88 Mouse)
   

   Use

   CRISPR

   Tags

   3XFLAG and GFP

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Feb. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLKO-PTEN-shRNA-3001

   Plasmid

   #25639

   Depositor

   Todd Waldman

   Article

   Kim et al Mol Cell Biol. 2007 Jan . 27(2):662-77.

   Insert

   PTEN-shRNA-3001 (PTEN Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   June 21, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pFUGW-hGtACR2-EYFP

   Plasmid

   #67877

   Purpose

   Mammalian expression construct of human codon-adapted anion channelrhodopsin 2 from Guillardia theta fused in frame to EYFP via a NotI site in the pFUGW vector backbone

   Depositor

   John Spudich

   Article

   Govorunova et al Science. 2015 Jun 25. pii: aaa7484.

   Insert

   hGtACR2
   

   Use

   Lentiviral

   Tags

   EYFP

   Expression

   Mammalian

   Promoter

   UbC

   Available Since

   Aug. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. TLCV2-sgHPDL #9

   Plasmid

   #174166

   Purpose

   knock out HPDL in human cell lines

   Depositor

   Michael Pacold

   Article

   Banh et al Nature. 2021 Sep 1. pii: 10.1038/s41586-

   Insert

   sgRNA against HPDL
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Sept. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. PX458_ZNF644_2

   Plasmid

   #86306

   Purpose

   Encodes gRNA for 3' target of human ZNF644

   Depositor

   Eric Mendenhall, Richard M. Myers

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   gRNA against ZNF644 (ZNF644 Human)
   

   Use

   CRISPR

   Available Since

   Jan. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pFUGW-hGtACR1-EYFP

   Plasmid

   #67795

   Purpose

   Mammalian expression construct of human codon-adapted anion channelrhodopsin 1 from Guillardia theta fused in frame to EYFP via a NotI site in the pFUGW vector backbone

   Depositor

   John Spudich

   Article

   Govorunova et al Science. 2015 Jun 25. pii: aaa7484.

   Insert

   hGtACR1
   

   Use

   Lentiviral

   Tags

   EYFP

   Expression

   Mammalian

   Available Since

   Aug. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pENTR-PpU6P-sgRNA-L3L2

   Plasmid

   #113740

   Purpose

   Gateway entry vector containing attL3 and attL2 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.

   Depositor

   Magdalena Bezanilla

   Article

   Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

   Insert

   PpU6P::sgRNA
   

   Use

   CRISPR; Gateway entry vector

   Promoter

   P. patens U6 promoter

   Available Since

   Sept. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pENTR-PpU6P-sgRNA-L1L4

    Plasmid

    #113736

    Purpose

    Gateway entry vector containing attL1 and attL4 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.

    Depositor

    Magdalena Bezanilla

    Article

    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

    Insert

    PpU6P::sgRNA
    

    Use

    CRISPR; Gateway entry vector

    Promoter

    P. patens U6 promoter

    Available Since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTX1-SMN1-exon7-ESE1

    Plasmid

    #126041

    Purpose

    In-vitro-transcription of SMN1-exon7-ESE1 RNA in NMR tube for "Systems NMR" analysis

    Depositor

    Frédéric Allain

    Article

    Nikolaev et al Nat Methods. 2019 Aug;16(8):743-749. doi

    Insert

    SMN1 exon7 ESE1
    

    Available Since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pENTR-PpU6P-sgRNA-L1L2

    Plasmid

    #113735

    Purpose

    Gateway entry vector containing attL1 and attL2 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.

    Depositor

    Magdalena Bezanilla

    Article

    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

    Insert

    PpU6P::sgRNA
    

    Use

    CRISPR; Gateway entry vector

    Promoter

    P. patens U6 promoter

    Available Since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pENTR-PpU6P-sgRNA-L5L2

    Plasmid

    #113738

    Purpose

    Gateway entry vector containing attL5 and attL2 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.

    Depositor

    Magdalena Bezanilla

    Article

    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

    Insert

    PpU6P::sgRNA
    

    Use

    CRISPR; Gateway entry vector

    Promoter

    P. patens U6 promoter

    Available Since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pENTR-PpU6P-sgRNA-R4R3

    Plasmid

    #113739

    Purpose

    Gateway entry vector containing attR4 and attR3 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.

    Depositor

    Magdalena Bezanilla

    Article

    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

    Insert

    PpU6P::sgRNA
    

    Use

    CRISPR; Gateway entry vector

    Promoter

    P. patens U6 promoter

    Available Since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pENTR-PpU6P-sgRNA-L5L4

    Plasmid

    #113741

    Purpose

    Gateway entry vector containing attL5 and attL4 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.

    Depositor

    Magdalena Bezanilla

    Article

    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

    Insert

    PpU6P::sgRNA
    

    Use

    CRISPR; Gateway entry vector

    Promoter

    P. patens U6 promoter

    Available Since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pENTR-PpU6P-sgRNA-L1R5

    Plasmid

    #113737

    Purpose

    Gateway entry vector containing attL1 and attR5 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.

    Depositor

    Magdalena Bezanilla

    Article

    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

    Insert

    PpU6P::sgRNA
    

    Use

    CRISPR; Gateway entry vector

    Promoter

    P. patens U6 promoter

    Available Since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pColE1_sgRNA_M1S_AmpR

    Plasmid

    #119153

    Purpose

    Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with one mismatch in the seed region

    Depositor

    Vincent Noireaux

    Article

    Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

    Insert

    sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with one mismatch in the seed region
    

    Use

    Crispr

    Promoter

    J23119

    Available Since

    Dec. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pColE1_sgRNA_M1NS_AmpR

    Plasmid

    #119155

    Purpose

    Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with one mismatch in the non-seed region

    Depositor

    Vincent Noireaux

    Article

    Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

    Insert

    sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with one mismatch in the non-seed region
    

    Use

    Crispr

    Promoter

    J23119

    Available Since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pgRNAtet-phaZ

    Plasmid

    #169874

    Purpose

    Single guide RNA plasmid for Cas9 targeting phaZ in P. putida

    Depositor

    Brian Pfleger

    Article

    Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

    Insert

    sgRNA towards phaZ in Pseudomonas putida
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Available Since

    July 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pX459_gRNA pAS single_hspCas9

    Plasmid

    #193311

    Purpose

    Cas9 from S. pyogenes and U6-pAS single sgRNA (V2.0)

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Methods Mol Biol. 2023;2676:169-180. doi

    Insert

    Cas9 from S. pyogenes and U6-pAS single sgRNA (V2.0)
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    not applicable

    Available Since

    Dec. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge