218,568 results

1. pREP-8xARE-GFP-SV40-BFP

   Plasmid

   #134910

   Purpose

   NRF2 reporter plasmid with GFP under control of 8 ARE elements and constitutively transcribed BFP

   Depositor

   Markus Landthaler

   Article

   Wyler et al Nat Commun. 2019 Oct 25;10(1):4878. doi:

   Inserts

   8xARE- minimal promoter - GFP
   TagBFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   8xARE - minimal promoter and SV40

   Available since

   Jan. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRN11

   Plasmid

   #84455

   Purpose

   shuttle plasmid for sarA P1-mCherry expression

   Depositor

   Reindert Nijland

   Article

   de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

   Insert

   mCherry
   

   Use

   Shuttle vector e.coli-s.aureus, expression of sgf…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. gRNA_Cloning Vector

   Plasmid

   #41824

   Purpose

   An empty gRNA expression vector, used to create a gRNA to a specific sequence.

   Depositor

   George Church

   Article

   Mali et al Science. 2013 Feb 15;339(6121):823-6. do

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   AflII site for Gibson Assembly

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. PB-TAC-ERP2

   Plasmid

   #80478

   Purpose

   All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry and constitutive rtTA and puromycin resistance)

   Depositor

   Knut Woltjen

   Article

   Kim et al Methods Mol Biol. 2016;1357:111-31. doi:

   Type

   Empty backbone

   Use

   Piggybac transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pT/Caggs-NRASV12

   Plasmid

   #20205

   Depositor

   John Ohlfest

   Article

   Wiesner et al Cancer Res. 2009 Jan 15. 69(2):431-9.

   Insert

   NRas (NRAS Human)
   

   Use

   Transposon

   Tags

   Expression

   Mutation

   G12V

   Promoter

   Available since

   Feb. 4, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. PB_rtTA_BsmBI

   Plasmid

   #126028

   Purpose

   PiggyBac cargo vector expressing rtTA, contains BsmBI sites for sgRNA cloning

   Depositor

   Mauro Calabrese

   Article

   Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

   Type

   Empty backbone

   Use

   CRISPR; Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   June 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-hSyn-Cre-P2A-dTomato (AAV PHP.eB)

   Viral Prep

   #107738-PHPeB

   Purpose

   Ready-to-use AAV PHP.eB particles produced from pAAV-hSyn-Cre-P2A-dTomato (#107738). In addition to the viral particles, you will also receive purified pAAV-hSyn-Cre-P2A-dTomato plasmid DNA. hSyn-driven expression of Cre and dTomato (physically separate). These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Rylan Larsen

   Promoter

   Syn

   Tags

   dTomato (physically separate, not a fusion protein)

   Available since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

8. pInt-ts

   Plasmid

   #66076

   Purpose

   The pInt-ts plasmid [Haldimann2001] is the lambda helper plasmid necessary for the genome integration of the burden monitor lambda version

   Depositor

   Tom Ellis

   Article

   Ceroni et al Nat Methods. 2015 May;12(5):415-8. doi:

   Insert

   lambda integrase
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-hSyn-DIO-hM3D(Gq)-mCherry

   Plasmid

   #44361

   Purpose

   Double floxed Gq-coupled hM3D DREADD fused with mCherry under the control of human synapsin promoter.

   Depositor

   Bryan Roth

   Article

   Krashes et al J Clin Invest. 2011 Apr;121(4):1424-8. d

   Has Service

   AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

   Insert

   hM3D(Gq)-mCherry
   

   Use

   AAV; Adeno associated viral vector

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   human Synapsin 1

   Available since

   May 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCFD6

    Plasmid

    #73915

    Purpose

    tissue-specific multiplex gRNA plasmid

    Depositor

    Simon Bullock

    Article

    Port et al Nat Methods. 2016 Oct;13(10):852-4. doi:

    Insert

    :tRNA:gRNA:tRNA:gRNA:tRNA
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    UAS-DSCP

    Available since

    Aug. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSP2-96-merTetO-EFS-BLaR

    Plasmid

    #118713

    Purpose

    Contains an optimized 96-mer TetO repeat for imaging of desired loci

    Depositor

    Huimin Zhao

    Article

    Tasan et al Nucleic Acids Res. 2018 Jun 15. pii: 503

    Inserts

    Blasticidin resistance gene
    EFS-NS promoter
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS-NS

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET29a-human histone 4-in-One

    Plasmid

    #223237

    Purpose

    Polycistronic bacterial coexpression construct for human His-Sumo-H2A, H2B, H3, and Dual-StrepII-H4

    Depositor

    Weixing Zhao

    Inserts

    His6-VSVG-Histone H2A
    Histone H2B
    Histone H3
    Histone H4-TEV-His6-Dual StrepII
    

    Use

    Tags

    Dual StrepII, His6, TEV protease site, and VSVG

    Expression

    Bacterial

    Mutation

    A103G

    Promoter

    T7

    Available since

    Aug. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-Flpe

    Plasmid

    #13787

    Purpose

    Mammalian expression of enhanced form of Flp recombinase

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

    Insert

    Flpe
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 20, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCXLE-hOCT3/4-shp53-F

    Plasmid

    #27077

    Purpose

    Integration-free (episomal) expression of human OCT3/4 and shRNA against p53

    Depositor

    Shinya Yamanaka

    Article

    Okita et al Nat Methods. 2011 May;8(5):409-12

    Insert

    OCT3/4 (POU5F1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. LVXN-Neo-NSD2

    Plasmid

    #86010

    Purpose

    express wild-type NSD2 in mammalian cells

    Depositor

    Darrin Stuart

    Article

    Jaffe et al Nat Genet. 2013 Nov;45(11):1386-91. doi:

    Insert

    NSD2 (NSD2 Human)
    

    Use

    Lentiviral

    Tags

    Flag tag D Y K D D D D K

    Expression

    Bacterial

    Mutation

    K1002R (please see depositor comments below)

    Promoter

    CMV

    Available since

    Jan. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBPGAL80Uw-6

    Plasmid

    #26236

    Depositor

    Gerald Rubin

    Article

    Pfeiffer et al Genetics. 2010 Aug 9. ():.

    Insert

    GAL80 (GAL80 Budding Yeast)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 4, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBabe puro HA PIK3CA H1047R

    Plasmid

    #12524

    Purpose

    Retroviral expression of HA tagged phosphoinositide 3-kinase H1047R (Tumor-derived allele)

    Depositor

    Jean Zhao

    Article

    Zhao et al Proc Natl Acad Sci U S A. 2005 Dec 20. 1

    Insert

    PIK3CA H1047R (PIK3CA Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    H1047R. Tumor-derived allele.

    Promoter

    Available since

    Aug. 18, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMXS-mitoGshF-blasticidin

    Plasmid

    #210314

    Purpose

    Expresses mitochondrially targeted, humanized Streptococcus thermophilus GshF in mammalian cells

    Depositor

    Kivanc Birsoy

    Article

    Wang et al Nature. 2021 Nov;599(7883):136-140. doi:

    Insert

    Bifunctional glutamate-cysteine ligase/glutathione synthetase GshF (gshAB Streptococcus thermophilus)
    

    Use

    Retroviral

    Tags

    FLAG peptide, Homo sapiens ACO2 mitochondrial tar…

    Expression

    Mutation

    Codon optimized for expression in human cells

    Promoter

    Available since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-hSyn-hChR2(H134R)-EYFP

    Plasmid

    #26973

    Purpose

    AAV expression of humanized ChR2 with H134R mutation fused to EYFP driven by human Synapsin I promoter for optogenetic excitation

    Depositor

    Karl Deisseroth

    Has Service

    AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, and AAV9

    Insert

    hChR2(H134R)
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    H134R

    Promoter

    hSyn

    Available since

    Feb. 4, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. TATA-GFP

    Plasmid

    #110833

    Purpose

    negative control

    Depositor

    Michael Lewis

    Article

    Wei et al Stem Cells. 2014 Oct;32(10):2571-82. doi

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge