228,568 results

1. pAV-U6+27-Tornado-F30-Broccoli-aptNFkB#5

   Plasmid

   #124362

   Purpose

   Overexpression of bifunctional circular RNA (Broccoli fluorescence and NFkB binding)

   Depositor

   Samie Jaffrey

   Article

   Litke et al Nat Biotechnol. 2019 Apr 8. pii: 10.1038

   Insert

   Tornado-F30-Broccoli-aptNFkB#5
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6+27

   Available since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCI-neo-cOVA

   Plasmid

   #25097

   Depositor

   Maria Castro

   Article

   Yang et al Proc Natl Acad Sci U S A. 2010 Mar 9. 10

   Insert

   cytoplasmic ovalbumin
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   July 1, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. bZiPro

   Plasmid

   #86846

   Purpose

   express NS2B-NS3 protease in E.coli

   Depositor

   Dahai Luo

   Article

   Zhang et al Science. 2016 Dec 8. pii: aai9309.

   Inserts

   NS3 1-177
   NS2B45-96
   

   Use

   Tags

   a hexahistidine tag and a thrombin cleavage site

   Expression

   Bacterial

   Mutation

   NS2B cofactor region (residues 45–96) and NS3 pro…

   Promoter

   T7

   Available since

   March 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. Multiplex Yeast Toolkit (MYT)

   Plasmid Kit

   #1000000229

   Purpose

   The Multiplex Yeast Toolkit (MYT) is a collection of 96 plasmids for genetically engineering the yeast, Saccharomyces cerevisiae.

   Depositor

   Tom Ellis

   Available since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

5. gTBEv5

   Plasmid

   #220621

   Purpose

   vector for encoding an engineered glycosylase-based thymine (T) base editor (gTBEv5) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter

   Depositor

   Huawei Tong

   Article

   Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:

   Insert

   nSpCas9(Nterm)-XTEN-hUNG2-XTEN-Cas9(Cterm)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   hUNG2(del1-88;Y156A,A214T,Q259A,Y284D), SpCas9(D1…

   Promoter

   hU6, EF1a

   Available since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-TFEB-WT-MYC (#647)

   Plasmid

   #99955

   Purpose

   mammalian expression

   Depositor

   James Brugarolas

   Article

   Vega-Rubin-de-Celis et al Autophagy. 2017 Mar 4;13(3):464-472. doi

   Insert

   TFEB (TFEB Human)
   

   Use

   Tags

   6xHis and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHR-SFFV-KRAB-dCas9-P2A-mCherry

   Plasmid

   #60954

   Purpose

   2nd Generation Lentiviral vector. Expresses an N-terminal KRAB-dCas9 fusion protein and mCherry

   Depositor

   Jonathan Weissman

   Article

   Gilbert et al Cell. 2014 Oct 23;159(3):647-61. doi: 10

   Insert

   KRAB-dCas9-P2A-mCherry fusion
   

   Use

   CRISPR and Lentiviral

   Tags

   2x NLS, HA, KRAB domain, and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   Jan. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV/mIba1.GFP.miR-9.T.miR-129-2-3p.T.WPRE.miR-9.T.miR-129-2-3p.T.SV40pA

   Plasmid

   #226475

   Purpose

   Improved microglia-targeted transgene expression

   Depositor

   Hirokazu Hirai

   Inserts

   mIba1 promoter, 1.7-kb, microglia-specific promoter
   target sequences for miR-9x4 & miR-129-2-3px4 *2
   EGFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. LRRK2-WT

   Plasmid

   #17609

   Depositor

   Ted Dawson

   Article

   West et al Proc Natl Acad Sci U S A. 2005 Nov 15;10

   Insert

   LRRK2 (LRRK2 Human)
   

   Use

   Tags

   His and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 31, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-NLS-HA-Dre

    Plasmid

    #51272

    Purpose

    Component of the Co-Driver binary site-specific recombinase system, expression of the site-specific recombinase Dre (mouse codon-optimized)

    Depositor

    Pawel Pelczar

    Article

    Hermann et al Nucleic Acids Res. 2014 Jan 9.

    Insert

    Dre
    

    Use

    Tags

    NLS-HA

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. Apple-53BP1trunc

    Plasmid

    #69531

    Purpose

    Fluorescent reporter for DNA damage

    Depositor

    Ralph Weissleder

    Article

    Yang et al Sci Rep. 2015 May 18;5:10129. doi: 10.10

    Insert

    53BP1 (TP53BP1 Human)
    

    Use

    Lentiviral

    Tags

    Apple fluorescent protein

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti sfGFP-LAMP1-mCherry (pHLARE)

    Plasmid

    #164478

    Purpose

    Lysosomal pH biosensor consisting of sfGFP-rat Lamp1-mCherry. Plasmid for lentivirus production.

    Depositor

    Diane Barber

    Article

    Webb et al Mol Biol Cell. 2020 Nov 25:mbcE20060383.

    Insert

    LAMP1 (Lamp1 Rat)
    

    Use

    Lentiviral

    Tags

    mCherry and superfolder GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pIRES2-EGFP-SHP2WT

    Plasmid

    #12283

    Depositor

    Anton Bennett

    Article

    Kolli et al J Biol Chem. 2004 Jul 9. 279(28):29374-8

    Insert

    SHP-2WT (PTPN11 Human)
    

    Use

    Tags

    Myc (not canonical: KLISEEDL)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. gCBEv3

    Plasmid

    #220618

    Purpose

    vector for encoding an engineered glycosylase-based cytosine (C) base editor (gCBEv3) driven by EF1a promoter, guide RNAs compatible with SpG driven by hU6, mCherry driven by CBh promoter

    Depositor

    Huawei Tong

    Article

    Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:

    Insert

    nSpCas9(Nterm)-XTEN-hUNG2-XTEN-Cas9(Cterm)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    hUNG2(del1-88;N213D,K184A,A214V), SpCas9(D10A)

    Promoter

    hU6, EF1a

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX330-gRNA

    Plasmid

    #158973

    Purpose

    A human codon-optimized SpCas9 and chimeric guide RNA (gRNA3) expression plasmid.

    Depositor

    Charles P. Lai

    Article

    Chien et al Nucleic Acids Res. 2020 Aug 14. pii: 589

    Insert

    gRNA3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEGFP-N1-STAT3

    Plasmid

    #111934

    Purpose

    Expresses human STAT3 fused to GFP in mammalian cells

    Depositor

    Geert van den Bogaart

    Article

    Verboogen et al J Mol Cell Biol. 2018 Jul 16. pii: 50546

    Insert

    Signal transducer and activator of transcription 3 (STAT3 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. mApple-Alpha-V-Integrin-25

    Plasmid

    #54866

    Purpose

    Localization: Focal Adhesions, Excitation: 568, Emission: 592

    Depositor

    Michael Davidson

    Insert

    Alpha-V Integrin (ITGAV Human)
    

    Use

    Tags

    mApple

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-PE-Plus

    Plasmid

    #214079

    Purpose

    Mammalian expression of SpCas9 PEmax prime editor with P2A-human P53DD and IRES-human MLH1dn

    Depositor

    Ting Zhou

    Article

    Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

    Insert

    PE-Plus
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCW57.1_AOX-FLAG

    Plasmid

    #177984

    Purpose

    All-in-one dox-inducible expression of Alternative Oxidase

    Depositor

    Jessica Spinelli, David Sabatini

    Article

    Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

    Insert

    AOX
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Codon optimized cDNA for expression in human cells

    Promoter

    TRE

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-hSyn-DIO-hM4D(Gi)-mCherry

    Plasmid

    #44362

    Purpose

    Double floxed Gi-coupled hM4D DREADD fused with mCherry under the control of human synapsin promoter.

    Depositor

    Bryan Roth

    Article

    Krashes et al J Clin Invest. 2011 Apr;121(4):1424-8. d

    Has Service

    AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    hM4D(Gi)-mCherry
    

    Use

    AAV; Adeno associated viral vector

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    human Synapsin 1

    Available since

    April 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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