Addgene

We narrowed to 39,117 results for: Eras

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pLVUT-spC3GFP

Plasmid

#225487

Purpose

Tet-on lentiviral vector for controlled expression of a secretable and permeable form of C. botulinum exoenzyme C3 transferase fused with EGFP

Depositor

Robert Gross

Article

Gutekunst et al Neuroscience. 2016 Dec 17;339:308-318. d

Insert

Secretable and permeable exoenzyme C3 transferase

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Amino acids 30-251

Promoter

Human ubiquitin C

Available since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-IFT22

Plasmid

#218720

Purpose

Expresses N-terminally EGFP-tagged IFT22 in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Katoh et al J Biol Chem. 2016 Mar 15. pii: jbc.M116.

Insert

IFT22 (RABL5 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 NKX6-2-Venus R200W

Plasmid

#209179

Purpose

Mammalian expression of SPAX8-related missense mutation (R200W) in human NKX6-2 fused with the Venus fluorescent protein

Depositor

Federico Herrera

Article

Ferreira-Peralta et al Biochim Biophys Acta Mol Basis Dis. 2023

Insert

NKX6-2 (R200W) fused with Venus (NKX6-2 Human)

Use

Tags

Venus

Expression

Mammalian

Mutation

NKX6-2 (R200W)

Promoter

CMV

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 NKX6-2-Venus S246A/T252A

Plasmid

#209180

Purpose

Mammalian expression of human NKX6-2 T252D phosphoresistant mutant

Depositor

Federico Herrera

Article

Ferreira-Peralta et al Biochim Biophys Acta Mol Basis Dis. 2023

Insert

NKX6-2 (S246A/T252A) fused with Venus (NKX6-2 Human)

Use

Tags

Venus

Expression

Mammalian

Mutation

NKX6-2 (S246A/T252D)

Promoter

CMV

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 NKX6-2-Venus L163V

Plasmid

#209178

Purpose

Mammalian expression of SPAX8-related missense mutation (L163V) in human NKX6-2 fused with the Venus fluorescent protein

Depositor

Federico Herrera

Article

Ferreira-Peralta et al Biochim Biophys Acta Mol Basis Dis. 2023

Insert

NKX6-2 (L163V) fused with Venus (NKX6-2 Human)

Use

Tags

Venus

Expression

Mammalian

Mutation

NKX6-2 (L163V)

Promoter

CMV

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 NKX6-2-Venus Y238F

Plasmid

#209181

Purpose

Mammalian expression of human NKX6-2 T252D phosphoresistant mutant

Depositor

Federico Herrera

Article

Ferreira-Peralta et al Biochim Biophys Acta Mol Basis Dis. 2023

Insert

NKX6-2 (Y238F) fused with Venus (NKX6-2 Human)

Use

Tags

Venus

Expression

Mammalian

Mutation

NKX6-2 (Y238F)

Promoter

CMV

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pSKB3-SaGGR-F219L (JBEI-7186)

Plasmid

#110151

Purpose

pSKB3 with TEV-cleavable, N-terminal His-tagged SaGGR-F219L mutant gene

Depositor

Jay Keasling

Article

Kung et al Structure. 2014 Jul 8;22(7):1028-36. doi

Insert

GGR

Use

Tags

TEV-cleavable His-tag

Expression

Bacterial

Mutation

SaGGR-F219L mutant gene

Promoter

T7

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pNLF1-C_UNG:1-313

Plasmid

#211093

Purpose

Mammalian expression of full-length human UNG. C-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

UNG:M1-L313 (UNG Human)

Use

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pNLF1-N_RFWD3:1-774

Plasmid

#210954

Purpose

Mammalian expression of full-length human RFWD3. N-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

RFWD3:M1-E774 (RFWD3 Human)

Use

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pNLF1-C_XPO1:1-1071

Plasmid

#211088

Purpose

Mammalian expression of full-length human XPO1. C-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

XPO1:M1-D1071 (XPO1 Human)

Use

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pNLF1-C_RBBP7:1-425

Plasmid

#211021

Purpose

Mammalian expression of full-length human RBBP7. C-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

RBBP7:M1-S425 (RBBP7 Human)

Use

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pNLF1-N_WDR12:1-423

Plasmid

#210948

Purpose

Mammalian expression of full-length human WDR12. N-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

WDR12:M1-A423 (WDR12 Human)

Use

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pNLF1-N_WDR91:1-747

Plasmid

#210998

Purpose

Mammalian expression of full-length human WDR91. N-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

WDR91:M1-A747 (WDR91 Human)

Use

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pNLF1-C_WDR91:1-747

Plasmid

#210999

Purpose

Mammalian expression of full-length human WDR91. C-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

WDR91:M1-A747 (WDR91 Human)

Use

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pNLF1-C_WDR41:1-459

Plasmid

#210991

Purpose

Mammalian expression of full-length human WDR41. C-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

WDR41:M1-V459 (WDR41 Human)

Use

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pNLF1-N_PWP1:1-501

Plasmid

#210974

Purpose

Mammalian expression of full-length human PWP1. N-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

PWP1:M1-S501 (PWP1 Human)

Use

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

E165D F448L G449R not annotated variants

Promoter

CMV

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pNLF1-C_MOV10:1-1003

Plasmid

#211086

Purpose

Mammalian expression of full-length human MOV10. C-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

MOV10:M1-L1003 (MOV10 Human)

Use

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pNLF1-N_RBBP7:1-425

Plasmid

#211020

Purpose

Mammalian expression of full-length human RBBP7. N-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

RBBP7:M1-S425 (RBBP7 Human)

Use

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pNLF1-N_WDR81:1-745

Plasmid

#211003

Purpose

Mammalian expression of human WDR81 fragment M1 to L745. N-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

WDR81:M1-L745 (WDR81 Human)

Use

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pNLF1-N_WDR81:1615-1941

Plasmid

#211000

Purpose

Mammalian expression of human WDR81 fragment G1615 to A1941. N-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

WDR81:G1615-A1941 (WDR81 Human)

Use

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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