We narrowed to 5,840 results for: pcas

1. pX330_ACTB-i4 sgRNA / hSpCas9

   Plasmid

   #172828

   Purpose

   Mammalian expression of a sgRNA targeting the intron 1 position 4 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Insert

   sgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Aug. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAGGS-S-B.1.617.2-FLAG

   Plasmid

   #177097

   Purpose

   B.1.617.2 variant S-protein with c-terminal FLAG tag

   Depositor

   Daniel Conway

   Insert

   S-protein B.1.617.2 (S SARS-CoV-2)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Nov. 2, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pCAG-Dcst1-3xFLAG

   Plasmid

   #183542

   Purpose

   Expression vector of mouse DC-STAMP domain containing 1 (Dcst1) tagged with 3xFLAG at C-terminus.

   Depositor

   Masahito Ikawa

   Article

   Noda et al Commun Biol. 2022 Apr 7;5(1):332. doi: 1

   Insert

   DC-STAMP domain containing 1 (Dcst1 Mouse)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   May 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-AkCas12b-2AeGFP

   Plasmid

   #121946

   Purpose

   Mammalian expression, Genome editing

   Depositor

   Wei Li

   Article

   Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

   Insert

   AkCas12b
   

   Tags

   3xHA tag

   Expression

   Mammalian

   Available Since

   Feb. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGL3-B-264;+80pCalb2

   Plasmid

   #66746

   Purpose

   luciferase reporter for Calb2 promoter (-264 to +80, WT)

   Depositor

   Emanuela Felley-Bosco, Rolf Stahel

   Article

   Kresoja-Rakic et al Oncotarget. 2016 Feb 1. doi: 10.18632/on

   Insert

   Calb2 promoter (-264bp+80) (CALB2 Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Promoter

   Calb2

   Available Since

   Jan. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGL3-B-419;+80pCalb2

   Plasmid

   #66747

   Purpose

   luciferase reporter for Calb2 promoter (-419 to +80, WT)

   Depositor

   Emanuela Felley-Bosco, Rolf Stahel

   Article

   Kresoja-Rakic et al Oncotarget. 2016 Feb 1. doi: 10.18632/on

   Insert

   Calb2 promoter (-419bp+80) (CALB2 Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Promoter

   Calb2

   Available Since

   Jan. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-nDsRedFL-miR124

   Plasmid

   #177806

   Purpose

   The pre-miR-124 sequence has been inserted into the EcoRV of pCAG-nDsRedFL-intron.

   Depositor

   Rikako Sanuki

   Article

   Suzuki et al Genes Cells. 2020 Nov;25(11):741-752. do

   Insert

   miR-124
   

   Expression

   Mammalian

   Available Since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAGGS-GCaMP2-actin

   Plasmid

   #18928

   Depositor

   Karel Svoboda

   Article

   Mao et al PLoS ONE. 2008 . 3(3):e1796.

   Insert

   GCaMP2-Actin (ACTB Chicken)
   

   Tags

   His

   Expression

   Mammalian

   Available Since

   Sept. 3, 2008

   Availability

   Academic Institutions and Nonprofits only

9. BII-gR-BtW-eSpCas9

   Plasmid

   #133358

   Purpose

   Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications). Provides gRNA and EspCas9 expression

   Depositor

   Blair Madison

   Article

   Fischer et al Am J Physiol Gastrointest Liver Physiol.

   Insert

   eSpCas9
   

   Tags

   Flag-tagged eSpCas9

   Expression

   Mammalian

   Mutation

   eSpCas9 mutations

   Promoter

   CMV enhancer and hEf1a (CpG free)

   Available Since

   Jan. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-AviMta2-3xFiBsd

    Plasmid

    #140964

    Purpose

    full-length wt Avi-Mta2-3XFLAG-iBsd cDNA w/CAG promoter and Blasticidin resistance, cloned from mouse ES cells

    Depositor

    Brian Hendrich

    Article

    Burgold et al EMBO J. 2019 Jun 17;38(12). pii: embj.20

    Insert

    metastasis-associated gene family, member 2 (Mta2 Mouse)
    

    Tags

    3xFLAG, Avi, and TEV

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCaSpeR-act5cB-GAL4QF

    Plasmid

    #61309

    Purpose

    Expresses chimeric transactivator GAL4_DBD:QF2w_AD in Drosophila

    Depositor

    Christopher Potter

    Article

    Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

    Insert

    GAL4_DBD::QF2w_AD
    

    Expression

    Insect

    Mutation

    Changed EK on C-terminus of QF2 to KKKK

    Available Since

    April 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. G1397 DddAtox-C–dSpCas9

    Plasmid

    #157836

    Purpose

    expresses split DddAtox-Cas9 construct in mammalian cells

    Depositor

    David Liu

    Article

    Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

    Insert

    bpNLS–G1397 DddAtox-C–dSpCas9–UGI–UGI–bpNLS
    

    Use

    CRISPR

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCas9–mKate2ps–CgRNA

    Plasmid

    #64955

    Purpose

    Control gRNA (GTCAAGGCACTCTTGCCTA)

    Depositor

    Leonidas Bleris

    Article

    Moore et al Nucleic Acids Res. 2015 Jan 30;43(2):129

    Insert

    Cas9–mKate2ps
    

    Expression

    Mammalian

    Available Since

    May 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-HA-NLGN4 D396*

    Plasmid

    #59321

    Purpose

    Expression plasmid of HA-tagged human Neuroligin 4 harboring a D396* nonsense mutation

    Depositor

    Peter Scheiffele

    Article

    Chih et al Hum Mol Genet. 2004 Jul 15;13(14):1471-7

    Insert

    NLGN4 D396* (NLGN4X Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    D396* nonsense mutation, rest of ORF deleted

    Promoter

    CAG

    Available Since

    June 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX330-Flag-dSpCas9

    Plasmid

    #92113

    Purpose

    Expression plasmid for human codon-optimized dead/inactive SpCas9 (without U6-sgRNA coding sequence)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    dead/inactive SpCas9 with FLAG tag
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    Cbh

    Available Since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAS9-mCherry-HIST1H4C

    Plasmid

    #66944

    Purpose

    CRISPaint target selector HIST1H4C

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

    Insert

    gRNA HIST1H4C
    

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. DN1-Gli3 in AINGpCAGGS

    Plasmid

    #121970

    Purpose

    medium Gli3 transcriptional activator

    Depositor

    James Briscoe

    Article

    Stamataki et al Genes Dev. 2005 Mar 1;19(5):626-41. doi:

    Insert

    DN2Gli3 (GLI3 Human)
    

    Use

    Chick electroporation

    Tags

    5xmyc

    Expression

    Mammalian

    Mutation

    N terminus deletion (nt1-864)

    Available Since

    April 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-Dcst2-3xHA

    Plasmid

    #183543

    Purpose

    Expression vector of mouse DC-STAMP domain containing 2 (Dcst2) tagged with 3xHA at C-terminus.

    Depositor

    Masahito Ikawa

    Article

    Noda et al Commun Biol. 2022 Apr 7;5(1):332. doi: 1

    Insert

    DC-STAMP domain containing 2 (Dcst2 Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    May 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-mGSDMD1-251

    Plasmid

    #111565

    Purpose

    Express ELANE-cleaved mouse GSDMD in mammalian cells

    Depositor

    Hongbo Luo

    Article

    Kambara et al Cell Rep. 2018 Mar 13;22(11):2924-2936.

    Insert

    Gasdermin D (Gsdmd Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

20. pCAG-RFP-int

    Plasmid

    #19822

    Depositor

    Zuoshang Xu

    Article

    Qiu et al BMC Biotechnol. 2008 Oct 7;8:77. doi: 10

    Type

    Empty backbone

    Use

    RNAi

    Expression

    Mammalian

    Available Since

    Nov. 21, 2008

    Availability

    Academic Institutions and Nonprofits only