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pRS416 Gal G294A YFP

Plasmid

#27448

Depositor

Aaron Gitler

Article

Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

Insert

TDP-43 (TARDBP Human)

Tags

YFP

Expression

Yeast

Mutation

Glycine 294 mutated to Alanine (G294A)

Available Since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only
pENO1-dTom-NATr

Plasmid

#52208

Purpose

Constitutive expression of Candida codon-optimized dTomato with NAT selectable marker

Depositor

Robert Wheeler

Article

Gratacap et al Dis Model Mech. 2013 Sep;6(5):1260-70. d

Insert

dTomato

Expression

Bacterial and Yeast

Mutation

codon optimized for Candida species

Promoter

ENO1

Available Since

March 24, 2014

Availability

Academic Institutions and Nonprofits only
mscv2.2-6mycFlaA

Plasmid

#60203

Purpose

6xMYC-tagged full-length FlaA from Legionella pneumophila, expressed under the constitutive, moderate-level retroviral LTR promoter for mammalian cell expression; IRES-GFP reporter downstream of FlaA

Depositor

Russell Vance

Article

Kofoed et al Nature. 2011 Aug 28;477(7366):592-5. doi

Insert

FlaA

Use

Retroviral

Tags

6xMYC

Expression

Mammalian

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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Mef2D-shRNA

Plasmid

#32900

Depositor

Michael Greenberg

Article

Flavell et al Science. 2006 Feb 17;311(5763):1008-12.

Insert

Mef2d (Mef2d Rat)

Use

RNAi

Expression

Mammalian

Promoter

H1-RNA

Available Since

Nov. 18, 2011

Availability

Academic Institutions and Nonprofits only
GAL FLAG25Q+ProGFPp303

Plasmid

#15572

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

htt 25Q + Pro (HTT Human)

Tags

FLAG and GFP

Expression

Yeast

Mutation

Proline-rich region present

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only

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pMIG-Aalpha E64G

Plasmid

#10885

Depositor

William Hahn

Article

Chen et al Cancer Res. 2005 Sep 15. 65(18):8183-92.

Insert

PP2A Aalpha (PPP2R1A Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

E64G

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only
pBAD-LSSmApple

Plasmid

#191259

Purpose

Bacterial expression plasmids for Long Stokes shift red fluorescent protein LSSmApple

Depositor

Robert Campbell

Article

Ejike et al Biochemistry. 2024 Jan 2;63(1):171-180.

Insert

LSSmApple

Tags

His tag

Expression

Bacterial

Available Since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pAH123

Plasmid

#66077

Purpose

The pAH123 plasmid [Haldimann2001] is the phi80 helper plasmid necessary for the genome integration of the burden monitor phi80 version

Depositor

Tom Ellis

Article

Ceroni et al Nat Methods. 2015 May;12(5):415-8. doi:

Insert

phi80 integrase

Available Since

July 27, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-tdTomato-WPRE

Plasmid

#133786

Purpose

Cre-dependent tdTomato fluorescent reporter for cell labeling

Depositor

Michael Stryker

Article

Sun et al Proc Natl Acad Sci U S A. 2019 Oct 22;11

Insert

pAAV-EF1a-DIO-tdTomato-WPRE

Use

AAV and Cre/Lox

Expression

Mammalian

Available Since

June 19, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ265E2

Plasmid

#164719

Purpose

Gateway entry clone (attL1 & attR5) A3A/Y130F-CBE_V01 for C-T base editing

Depositor

Yiping Qi

Article

Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

Insert

A3A(Y130F)-zCas9(D10A)-UGI

Use

CRISPR; Gateway compatible a3a/y130f-cbe_v01 entr…

Expression

Plant

Available Since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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426Gal-FUS

Plasmid

#29591

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS (FUS Human)

Expression

Yeast

Available Since

July 1, 2011

Availability

Academic Institutions and Nonprofits only
pIntegrase_9

Plasmid

#60580

Purpose

plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Integrase_9

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pBAD

Available Since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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Mef2A-shRNA

Plasmid

#32899

Depositor

Michael Greenberg

Article

Flavell et al Science. 2006 Feb 17;311(5763):1008-12.

Insert

Mef2a (Mef2a Rat)

Use

RNAi

Expression

Mammalian

Promoter

H1-RNA

Available Since

Nov. 18, 2011

Availability

Academic Institutions and Nonprofits only
pOSIP-KL-mcherry

Plasmid

#116947

Purpose

Integrating mCherry into lambda attB in E. coli chromosome.

Depositor

David Bikard

Article

Cui et al Nat Commun. 2018 May 15;9(1):1912. doi:

Insert

mcherry

Available Since

March 11, 2019

Availability

Academic Institutions and Nonprofits only

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pAG303-Gal-GA50

Plasmid

#84907

Purpose

induce expression of dipeptide repeat GA in yeast

Depositor

Aaron Gitler

Article

Jovicic et al Nat Neurosci. 2015 Sep;18(9):1226-9. doi

Insert

GA50 (C9orf72 Synthetic)

Tags

FLAG and Myc

Expression

Yeast

Promoter

GAL1

Available Since

Dec. 1, 2016

Availability

Academic Institutions and Nonprofits only

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mCardinal-H2B-C-10

Plasmid

#56162

Purpose

Localization: Nucleus/Histones, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

H2B (H2BC11 Human)

Tags

mCardinal

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available Since

March 4, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pOTTC1328 - pAAV EF1a HA-hM3D(Gq)

Plasmid

#89149

Purpose

An AAV packaging plasmid that expresses HA-tagged hM3D(Gq) excitatory DREADD from the EF1a promoter.

Depositor

Brandon Harvey

Article

Gomez et al Science. 2017 Aug 4;357(6350):503-507. d

Insert

hM3D(Gq)

Use

AAV

Tags

HA

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pRS416 Gal TDP43 WT

Plasmid

#27458

Depositor

Aaron Gitler

Article

Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

Insert

TDP-43 (TARDBP Human)

Expression

Yeast

Available Since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only
pRL-TK 4x wt

Plasmid

#11313

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

4 bulged binding sites for CXCR4 siRNA antisense

Use

Luciferase

Tags

Rr-luc

Available Since

Feb. 15, 2006

Availability

Academic Institutions and Nonprofits only
SlugCas9

Plasmid

#163793

Purpose

Expresses SlugCas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Hu et al Nucleic Acids Res. 2021 Mar 15. pii: 617

Type

Empty backbone

Use

AAV and CRISPR

Expression

Mammalian

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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