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pmScarlet_NES_C1

Plasmid

#85060

Purpose

In vivo visualization of the cytoplasm, but not the nucleus (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

NES

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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p2Flag CMV2-ZO-2

Plasmid

#27415

Depositor

Marius Sudol

Article

Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

Insert

Zonula Occludens 2 (TJP2 Human)

Tags

2xFlag

Expression

Mammalian

Available Since

March 15, 2011

Availability

Academic Institutions and Nonprofits only
pPM56

Plasmid

#22855

Depositor

Feroz Papa

Article

Merksamer et al Cell. 2008 Nov 28. 135(5):933-47.

Insert

eroGFP & UPR-RFP

Expression

Yeast

Available Since

Jan. 21, 2010

Availability

Academic Institutions and Nonprofits only
pAAV-TRE-mTurquoise2-WPRE

Plasmid

#104110

Purpose

AAV-mediated expression of mTurquoise2 under the TRE promoter.

Depositor

Takeshi Imai

Article

Sakaguchi et al eLife. 2018 Nov 20;7. pii: 40350. doi: 1

Insert

mTurquoise2

Use

AAV

Promoter

TRE

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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Flag-Paxillin

Plasmid

#15212

Depositor

Rick Horwitz

Article

Webb et al J Cell Sci. 2005 Nov 1. 118(Pt 21):4925-

Insert

Paxillin (PXN Chicken)

Tags

Flag

Expression

Mammalian

Available Since

Sept. 13, 2007

Availability

Academic Institutions and Nonprofits only
MLV Gag-CFP

Plasmid

#1812

Depositor

Walther Mothes

Article

Sherer et al Traffic 2003 Nov;4(11):785-801.

Insert

Gag (gag MuLV)

Tags

CFP

Expression

Mammalian

Available Since

May 19, 2005

Availability

Academic Institutions and Nonprofits only

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CSII-prEF1a-mCherry-3xNLS

Plasmid

#125262

Purpose

Fluorescent reporter for cell size

Depositor

Jan Skotheim

Article

Berenson et al Mol Biol Cell. 2019 Nov 15;30(24):2985-2

Insert

mCherry-3xNLS

Use

Lentiviral

Tags

3xNLS

Expression

Mammalian

Promoter

EF1a

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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Flag-Rab7A Q86L

Plasmid

#28050

Depositor

Qing Zhong

Article

Sun et al Proc Natl Acad Sci U S A. 2010 Nov 9. 10

Insert

Rab7A (RAB7A Human)

Tags

2xHA and 3xFlag

Expression

Mammalian

Mutation

Q86L

Available Since

June 22, 2011

Availability

Academic Institutions and Nonprofits only
pRG2

Plasmid

#104174

Purpose

sgRNA expression

Depositor

Jin-Soo Kim

Article

Kim et al Genome Biol. 2017 Nov 15;18(1):218. doi:

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-alpha4 wt

Plasmid

#80016

Purpose

Mammalian expression of human integrin alpha4

Depositor

Chinten James Lim

Article

Liu et al Mol Cell Biol. 2013 Nov;33(21):4334-45.

Insert

integrin alpha4 (ITGA4 Human)

Expression

Mammalian

Promoter

CMV

Available Since

July 12, 2016

Availability

Academic Institutions and Nonprofits only
pFF745

Plasmid

#61450

Purpose

IPTG-inducible SCRIBE_kanR(ON)

Depositor

Timothy Lu

Article

Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

Insert

SCRIBE_kanR(ON)

Use

Synthetic Biology

Promoter

pLacO

Available Since

Feb. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pCI-neo-myc-LC3-HA

Plasmid

#45244

Depositor

Tamotsu Yoshimori

Article

Kabeya et al EMBO J. 2000 Nov 1. 19(21):5720-8.

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Tags

HA and myc

Expression

Mammalian

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA6-ARID1A 1759-2285

Plasmid

#39476

Depositor

Ie-Ming Shih

Article

Guan et al Cancer Res. 2011 Nov 1;71(21):6718-27. E

Insert

ARID1A (ARID1A Human)

Tags

His and V5

Expression

Mammalian

Mutation

expresses ARID1A aa1759-2285

Available Since

Sept. 13, 2012

Availability

Academic Institutions and Nonprofits only
pLH-sgRNA-Sirius-8XPP7

Plasmid

#121940

Purpose

CRISPR-Sirius plasmid

Depositor

Thoru Pederson

Article

Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

Insert

sgRNA-Sirius-8XPP7

Use

CRISPR and Lentiviral

Tags

no

Expression

Mammalian

Promoter

human U6 promoter

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pET26-Ufd1-His

Plasmid

#21266

Depositor

Hemmo Meyer

Article

Bruderer et al J Biol Chem. 2004 Nov 26. 279(48):49609-

Insert

Ufd1 (Ufd1l Mouse)

Tags

His

Expression

Bacterial

Available Since

July 1, 2009

Availability

Academic Institutions and Nonprofits only

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mRFP-FKBP12-5ptpase domain

Plasmid

#67516

Purpose

Recruitable human type IV 5-phosphatase domain (214-644)

Depositor

Tamas Balla

Article

Varnai et al J Cell Biol. 2006 Nov 6;175(3):377-82.

Insert

FKBP12-5ptpase enzyme (INPP5E, FKBP1A Human)

Tags

mRFP

Mutation

INPP5E has C641A to destroy the C-terminal CAAX d…

Available Since

Sept. 9, 2015

Availability

Academic Institutions and Nonprofits only
AMPK-β1-FLAG

Plasmid

#40602

Depositor

Harvey Lodish

Article

Wong et al J Biol Chem. 2006 Nov 24;281(47):36434-4

Insert

AMPK-β1 (Prkab1 Mouse)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only
EB3N-VVDfast-mCl3-iLID

Plasmid

#190165

Purpose

Expresses EB3N-VVDfast-mCl3-iLID, a microtubule anchor used for optogenetic recruitment of SspB-mCh-p60, in mammalian cells.

Depositor

Anna Akhmanova

Article

Meiring et al Curr Biol. 2022 Nov 7;32(21):4660-4674.e

Insert

EB3N-VVDfast-mClover3-iLID

Tags

-VVDfast-mClover3-iLID

Expression

Mammalian

Mutation

1-189 aa from EB3

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pIGAhRC

Plasmid

#112510

Purpose

human Ah receptor and Arnt cDNAs expressed under control of Gal1,10 bidirectional promoter

Depositor

Charles Miller

Article

Miller CA et al Toxicol Appl Pharmacol. 1999 Nov 1;160(3

Insert

HIF1B, AhR (ARNT, AHR Human, Budding Yeast)

Use

Synthetic Biology

Expression

Yeast

Promoter

Gal1,10

Available Since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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pCEP-WAF1

Plasmid

#16450

Depositor

Bert Vogelstein

Article

el-Deiry et al Cell. 1993 Nov 19. 75(4):817-25.

Insert

WAF1 (CDKN1A Human)

Expression

Mammalian

Available Since

April 7, 2008

Availability

Academic Institutions and Nonprofits only