We narrowed to 13,620 results for: crispr cas9

1. AW01_pU.CAG.eSpCas9(1.1).rBGpA

   Plasmid

   #199255

   Purpose

   Expression construct encoding the high specificity eSpCas9(1.1) nuclease

   Depositor

   Manuel Goncalves

   Article

   Chen et al Nucleic Acids Res. 2016 Jun 8. pii: gkw5

   Insert

   High specificity eSpCas9(1.1) nuclease
   

   Use

   CRISPR

   Tags

   3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLS

   Expression

   Mammalian

   Mutation

   K848A K1003A R1060A

   Promoter

   CAG promoter

   Available Since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. 3xFLAG-dCas9/p-bacteria

   Plasmid

   #64325

   Purpose

   Expresses 3xFLAG-dCas9 in E. coli in a tetracycline-dependent manner for enChIP analysis to purify specific genomic regions of interest.

   Depositor

   Hodaka Fujii

   Article

   Fujita et al BMC Res Notes. 2018 Jun 14;11(1):387. do

   Insert

   3xFLAG-dCas9
   

   Use

   CRISPR; Tetracycline-inducible

   Tags

   3xFLAG

   Expression

   Bacterial

   Mutation

   D10A, H840A

   Promoter

   pLtetO-1

   Available Since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHAGE-TO-Sp dCas9-mVenusN

   Plasmid

   #191396

   Purpose

   SpdCas9 fused to N-terminal part of mVenus

   Depositor

   Albert Jeltsch

   Article

   Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

   Insert

   N-terminal part of split mVenus
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Dec. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSimpleII-NmCas9-FLAG

   Plasmid

   #47872

   Purpose

   FLAG tagged NmCas9 with no NLS.

   Depositor

   James Thomson, Erik Sontheimer

   Article

   Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.

   Insert

   NmCas9
   

   Use

   CRISPR

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. A3D-Cas9n-UGI

   Plasmid

   #119137

   Purpose

   Base editor made from APOBEC3D

   Depositor

   Reuben Harris

   Article

   Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

   Insert

   APOBEC3D-Cas9 nickase-UGI
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   None

   Promoter

   CMV

   Available Since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. A3F-Cas9n-UGI

   Plasmid

   #119138

   Purpose

   Base editor made from APOBEC3F

   Depositor

   Reuben Harris

   Article

   Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

   Insert

   APOBEC3F-Cas9 nickase-UGI
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   None

   Promoter

   CMV

   Available Since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. A3C-Cas9n-UGI

   Plasmid

   #119136

   Purpose

   Base editor made from APOBEC3C

   Depositor

   Reuben Harris

   Article

   Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

   Insert

   APOBEC3C-Cas9 nickase-UGI
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   None

   Promoter

   CMV

   Available Since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. A3G-Cas9n-UGI

   Plasmid

   #119139

   Purpose

   Base editor made from APOBEC3G

   Depositor

   Reuben Harris

   Article

   Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

   Insert

   APOBEC3G-Cas9 nickase-UGI
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   None

   Promoter

   CMV

   Available Since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBSKΔB-CBh-Cas9n-pA

   Plasmid

   #82617

   Purpose

   Expresses Cas9 nickase in mammalian cells

   Depositor

   Hiroshi Ochiai

   Article

   Seirin-Lee et al PLoS Comput Biol. 2019 Sep 11;15(9):e100

   Insert

   SpCas9n
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pG0A0-1-1-PBAD-dCas9

    Plasmid

    #208815

    Purpose

    Expresses arabinose-inducible dCas9, codon-optimized for Salmonella

    Depositor

    Marc Erhardt

    Article

    Naseri et al Commun Biol. 2023 Oct 3;6(1):1005. doi:

    Insert

    Arabinose-inducible dCas9 that is codon optimized for expression in Salmonella
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Arabinsoe-inducible promoter

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. dCas9-KRAB-MeCP2

    Plasmid

    #110821

    Purpose

    Improved dCas9 repressor-dCas9-KRAB-MeCP2

    Depositor

    George Church, Alejandro Chavez

    Article

    Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

    Insert

    dCas9-KRAB-MeCP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

    Promoter

    CMV

    Available Since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pB-CAGGS-dCas9-KRAB

    Plasmid

    #110822

    Purpose

    PiggyBac compatible plasmid expressing dCas9-KRAB

    Depositor

    George Church, Alejandro Chavez

    Article

    Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

    Insert

    dCas9-KRAB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

    Promoter

    CAGGS

    Available Since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCF140_Gag-SpyCas9-NLS

    Plasmid

    #225959

    Purpose

    CMV-Intron-Gag-SpyCas9-NLS. Expresses Gag-SpyCas9-NLS for VLP production.

    Depositor

    Christof Fellmann

    Article

    Ramadoss et al bioRxiv [Preprint]. 2024 Jun 26:2024.06.

    Insert

    CMV-Intron-Gag-SpyCas9-NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNOC_dCas9-KRAB_sgRNA Td2

    Plasmid

    #176264

    Purpose

    pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged to the KRAB domain and Nlux and sgRNA targeting the fluorescent gene tdtomato with spacer 2

    Depositor

    John van der Oost

    Article

    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

    Insert

    dead spCas9
    

    Use

    CRISPR, Luciferase, and Synthetic Biology ; Expre…

    Tags

    Kruppel associated box domain and luciferase

    Expression

    Mutation

    H840A and D10A mutations on spCas9 to inactivate …

    Promoter

    Available Since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPB-CAG-dCas9-VPR-wpA-pPuroTK

    Plasmid

    #214127

    Purpose

    Gene expression by Piggybac transposase in mammalian cells

    Depositor

    Sho Yoshimatsu

    Insert

    dCas9-VPR
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Codon-optimized for mammalian cells

    Promoter

    Available Since

    Feb. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMEG_YLCas9_empty

    Plasmid

    #117826

    Purpose

    CRISPR/Cas9 plasmid for GoldenMOCS in Y. lipolytica

    Depositor

    Michael Sauer

    Article

    Egermeier et al FEMS Microbiol Lett. 2019 Jan 29. pii: 5

    Insert

    YlpTEF_hCas9_YlCyc1TT
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available Since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSSBIO32_dCas9_RPA1786

    Plasmid

    #240489

    Purpose

    CRISPRi-mediated Knockdown of RPA1786

    Depositor

    Rajib Saha

    Insert

    Cas9 (cas9 Streptococcus pyogenes)
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D10A, H840A

    Promoter

    Plac

    Available Since

    Aug. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBP_dCas9

    Plasmid

    #190131

    Purpose

    Level 0 MoClo plasmid containing dCas9

    Depositor

    Veronica Godoy-Carter

    Article

    Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAGM4723:Ble:Cas9

    Plasmid

    #112208

    Purpose

    Golden Gate Level 2 construct encoding Cas9-YFP, Zeocin resistance.

    Depositor

    Thomas Mock

    Inserts

    Cas9
    SheBle
    

    Use

    CRISPR; Diatom, phaeodactylum

    Tags

    Phaeodactylum fcp promoter and terminator and YFP

    Expression

    Mutation

    Phaeodactylum Fcp promoter

    Promoter

    Phaeodactylum Fcp

    Available Since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCSD_SpCas9MT3-FKBP_SL

    Plasmid

    #107302

    Purpose

    Expresses R1335K mutant SpCas9 fused to FKBP in mammalian cells

    Depositor

    Scot Wolfe

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    FKBP

    Expression

    Mammalian

    Mutation

    R1335K and fused to FKBP

    Promoter

    CMV IE94

    Available Since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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