163,819 results

1. pET28a-LSSmOrange-CNA35

   Plasmid

   #61605

   Purpose

   bacterial expression of N-terminal fusion protein of CNA35 with LSSmOrange

   Depositor

   Maarten Merkx

   Article

   Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

   Insert

   LSSmOrange and CNA35
   

   Use

   Tags

   His tag

   Expression

   Mutation

   Promoter

   T7

   Available since

   March 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCeru-N1 cyclin A (1557)

   Plasmid

   #61842

   Purpose

   mammalian expression of cyclin A with cerulean fusion

   Depositor

   Jonathon Pines

   Insert

   cyclin A (CCNA2 Human)
   

   Use

   Tags

   Cerulean

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pOpen-PstIM

   Plasmid

   #165536

   Purpose

   Methylase that recognizes the dsDNA sequence CTGCAG, methylates on A-5 on both strands, and protects DNA from cleavage by PstI.

   Depositor

   Jennifer Molloy, FreeGenes Project, Drew Endy

   Insert

   M.PstI
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 5, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pGL0_154 [pCD6 ORI]

   Plasmid

   #210676

   Purpose

   Level 0 part

   Depositor

   Nili Ostrov, Charlie Gilbert, Cultivarium Tools

   Insert

   pCD6 ORI
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBIG1a

   Plasmid

   #80611

   Purpose

   pBIG1a - Step1 vector a (biGBac system for expression of protein complexes in insect cells)

   Depositor

   Jan-Michael Peters

   Article

   Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

   Type

   Empty backbone

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA3 V5 mDispatched WT 2x HA

   Plasmid

   #126410

   Purpose

   Expression of V5 Dispatched WT HA in mammalian cell lines.

   Depositor

   Stacey Ogden

   Article

   Stewart et al Elife. 2018 Jan 23;7. pii: 31678. doi: 1

   Insert

   m-Dispatched1 (Disp1 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   V5 iserted between A106 and A107

   Promoter

   Available since

   June 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV-EF1a-DIO-GCaMP6f-P2A-nls-dTomato

   Plasmid

   #51083

   Purpose

   Cre dependent expression of GCaMP6f Ca sensor and physically separate nuclear localized dTomato fluorophore

   Depositor

   Jonathan Ting

   Has Service

   AAV Retrograde

   Insert

   GCaMP6f
   

   Use

   AAV

   Tags

   P2A-nls-dTomato

   Expression

   Mammalian

   Mutation

   Promoter

   hEF1a

   Available since

   Feb. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. ERroGFP_iE_pCDNA3

   Plasmid

   #47954

   Purpose

   Mammalian expression vector encoding for the ER targeted and FLAGM1 tagged redox probe roGFPiE (roGFP C147, E147b, C204)

   Depositor

   David Ron

   Article

   Avezov et al J Cell Biol. 2013 Apr 15;201(2):337-49.

   Insert

   ERroGFP
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   insertion E147B

   Promoter

   Available since

   Oct. 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pC0068 PspCas13 (B12) His6-TwinStrep-SUMO-BsaI

   Plasmid

   #115219

   Purpose

   Expresses PspCas13b from a SUMO-tagged bacterial expression construct

   Depositor

   Feng Zhang

   Article

   Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

   Insert

   PspCas13
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 21, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. AAV-mDlx-jGCaMP8m-WPRE (AAV9)

    Viral Prep

    #176754-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from AAV-mDlx-jGCaMP8m-WPRE (#176754). In addition to the viral particles, you will also receive purified AAV-mDlx-jGCaMP8m-WPRE plasmid DNA. mDlx enhancer-driven expression of calcium sensor GCaMP8m in GABA-ergic interneurons. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Loren Looger

    Promoter

    mDlx

    Tags

    None

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only