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Showing: 3301 - 3350 of 11733 results

  9. pSBBi-Pur GFP-TM

    Plasmid
    #139185
    Purpose
    SB-transposon with constitutive bi-directional promoter. One side contains transmembrane-anchored GFP; the other side contains the puromycin resistance gene.
    Depositor
    Neal Devaraj
    Insert
    GFP-TM
    Use
    Transposon
    Expression
    Mammalian
    Mutation
    fused to membrane anchor
    Promoter
    EF1α
    Available Since
    March 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    139185-plasmid-map-sequence-id-309371

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  11. pGEX2TK-Num1-PH

    Plasmid
    #71369
    Purpose
    bacterial expression of PH domain for phosphoinositide binding assay
    Depositor
    Mark Lemmon
    Article
    Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.
    Insert
    Num1-PH
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Pleckstrin homology (PH) domain; aa2563-2692
    Promoter
    tac
    Available Since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only

  16. pRN3P-NLS-EGFP-Btgh-3NLS

    Plasmid
    #194469
    Purpose
    Encodes for bacterial O-GlcNAcase BtGH84 fused to enhanced GFP and NLSs for effective nuclear localization, in backbone for T3 In Vitro Transcription.
    Depositor
    Mathieu Boulard
    Article
    Formichetti et al Dev Cell 10 Jan 2024
    Insert
    BtGH84
    Use
    For in vitro transcription from t3 promoter after…
    Tags
    SV40 Nuclear Localization Signal, SV40 Nuclear Lo…
    Expression
    Bacterial
    Available Since
    Jan. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    194469-plasmid-map-sequence-id-384873

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  21. pDL1

    Plasmid
    #182263
    Purpose
    Gibson cloning vector for synthesis of single and double stranded RNA
    Depositor
    Daniel Lobo
    Article
    Wolff et al STAR Protoc. 2022 Jun 14;3(3):101458. do
    Type
    Empty backbone
    Use
    Gibson cloning vector for synthesis of single and…
    Promoter
    T3 and SP6 for sense and antisense riboprobe synt…
    Available Since
    June 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    182263-plasmid-map-sequence-id-360127

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  26. RHOU

    Plasmid
    #38885
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    RHOU (RHOU Human)
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    C191L
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  35. GPRC6A-DuET

    Plasmid
    #213299
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    GPRC6A (GPRC6A Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213299-plasmid-map-sequence-id-430843

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  39. pBtPIPLC His

    Plasmid
    #173808
    Purpose
    Expression and purification of the mature form of Bacillus thuringiensis phosphaatidyl inositol specific phospholipase C (PI-PLC), UniProt ID P08954 from E. coli BL21 CodonPlus (DE3) RIL cells
    Depositor
    Anne Gershenson, Mary F Roberts
    Article
    He et al J Biol Chem. 2015 Jul 31;290(31):19334-4
    Insert
    Bacillus thuringiensis 1-phosphatidylinositol phosphodiesterase
    Tags
    6XHis
    Expression
    Bacterial
    Mutation
    none
    Promoter
    T7
    Available Since
    Oct. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    173808-plasmid-map-sequence-id-345681

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Showing: 3301 - 3350 of 11733 results