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pED-hIRE1(I642G)

Plasmid

#16263

Depositor

Peter Walter

Article

Lin et al Science. 2007 Nov 9. 318(5852):944-9.

Insert

IRE1 (ERN1 Human)

Expression

Mammalian

Mutation

isoleucine to glycine mutation at residue 642

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only

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Rab8WT(Flag) in pcDNA3.1neo

Plasmid

#46783

Purpose

expression of WT Rab8a in mammalian cells

Depositor

Terry Hébert

Article

Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

Insert

RAB8A (RAB8A Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only
pAAV-UAS-CITRINE

Plasmid

#104839

Purpose

SPARK reporter gene

Depositor

Alice Ting

Article

Kim et al Elife. 2017 Nov 30;6. doi: 10.7554/eLife

Insert

Citrine

Use

AAV

Expression

Mammalian

Promoter

UAS

Available Since

Jan. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3 MDM2 1-400

Plasmid

#45754

Depositor

Robert Lefkowitz

Article

Shenoy et al Science. 2001 Nov 9;294(5545):1307-13. E

Insert

MDM2 1-400 (MDM2 Human)

Expression

Mammalian

Mutation

aa 1-400, lacks catalytic RING domain

Promoter

CMV

Available Since

Aug. 21, 2013

Availability

Academic Institutions and Nonprofits only
eMagB-iRFP-Mito

Plasmid

#162250

Purpose

Blue-light dependent heterodimerization system

Depositor

Pietro De Camilli

Article

Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

Insert

eMagB-iRFP-OMP25

Expression

Mammalian

Promoter

beta-actin promoter

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pmCherry-C1-mTrim21ΔRING-Box

Plasmid

#105517

Purpose

Expression of mCherry-tagged mouse Trim21 with a deleted RING-box in mammalian cells

Depositor

Melina Schuh

Article

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

TRIM21 (Trim21 Mouse)

Tags

mCherry

Expression

Mammalian

Mutation

deletion of RING domain (aa124-462)

Promoter

CMV

Available Since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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pLH-sgRNA-Sirius-4X(MS2-PP7)

Plasmid

#121941

Purpose

CRISPR-Sirius plasmid

Depositor

Thoru Pederson

Article

Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

Insert

sgRNA-Sirius-4X(MS2-PP7)

Use

CRISPR and Lentiviral

Tags

no

Expression

Mammalian

Promoter

human U6 promoter

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pAJM.657

Plasmid

#108525

Purpose

CymRAM + PCymRC-YFP Reporter. Cuma sensor for use in non-Marionette strain.

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

CymRAM + PCymRC-YFP

Expression

Bacterial

Mutation

CymR: S110G A171V

Available Since

Aug. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-FLCN

Plasmid

#72291

Purpose

overexpression

Depositor

David Sabatini

Article

Tsun et al Mol Cell. 2013 Nov 21;52(4):495-505. doi

Insert

FLCN (FLCN Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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cyclin D1-HA-pBabe-Hygro

Plasmid

#22703

Depositor

William Kaelin

Article

Zhang et al Cancer Cell. 2009 Nov 6. 16(5):413-24.

Insert

cyclin D1 (CCND1 Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

wt cDNA

Available Since

Dec. 3, 2009

Availability

Academic Institutions and Nonprofits only

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pNO183

Plasmid

#101068

Purpose

ppsR2-bphP1 bicistronic operon plasmid

Depositor

Jeffrey Tabor

Article

Ong et al ACS Synth Biol. 2017 Nov 1. doi: 10.1021

Inserts

ppsR2 (ppsR2 Rhodopseudomonas palustris CGA009)
bphP1

Use

Synthetic Biology

Expression

Bacterial

Mutation

insert guanine at position 430 to fix frameshift …

Available Since

Nov. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5-FLAG-S6K1

Plasmid

#100509

Purpose

Expresses FLAG tagged S6K1 in mammalian cells

Depositor

David Sabatini

Article

Gu et al Science. 2017 Nov 10;358(6364):813-818.

Insert

S6K1 (Rps6kb1 Rat)

Tags

FLAG

Expression

Mammalian

Available Since

Nov. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pSM1959

Plasmid

#41837

Depositor

Susan Michaelis

Article

Metzger et al J Biol Chem. 2008 Nov 21;283(47):32302-1

Insert

SEC63-mRFP

Tags

mRFP

Expression

Yeast

Available Since

Jan. 22, 2013

Availability

Academic Institutions and Nonprofits only

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pRK5-ATP6V1D-HAGST

Plasmid

#87932

Purpose

C-term HA-GST tagged overexpression

Depositor

David Sabatini

Article

Zoncu et al Science. 2011 Nov 4;334(6056):678-83.

Insert

ATP6V1D (ATP6V1D Human)

Tags

HA-GST

Expression

Mammalian

Available Since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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pDEST-mCherry-GJA1-20k-N1

Plasmid

#49861

Purpose

Encodes C-terminal fragment of human connexin 43 initiating at M213 with a V5 C-terminal fusion tag

Depositor

Robin Shaw

Article

Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13

Insert

GJA1-20k (GJA1 Human)

Tags

mCherry

Expression

Mammalian

Mutation

GJA1-20k corresponds to residues 213 to 382 to Co…

Promoter

CMV

Available Since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only

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pEB1029

Plasmid

#22066

Depositor

Emmanuelle Bouveret

Article

Battesti et al Proteomics. 2008 Nov . 8(22):4768-71.

Type

Empty backbone

Tags

T25-Flag

Expression

Bacterial

Available Since

Oct. 22, 2009

Availability

Academic Institutions and Nonprofits only

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PCMV-intron myc Rab6 T27N

Plasmid

#46782

Purpose

expression of T27N Rab6a in mammalian cells

Depositor

Terry Hébert

Article

Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

Insert

Rab6 T27N (RAB6A Human)

Tags

myc

Expression

Mammalian

Mutation

T27N dominant negative

Promoter

CMV

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only
Halo-Rab6A

Plasmid

#190171

Purpose

Expresses HaloTag-Rab6A in mammalian cells.

Depositor

Anna Akhmanova

Article

Meiring et al Curr Biol. 2022 Nov 7;32(21):4660-4674.e

Insert

HaloTag-Rab6A

Tags

HaloTag

Expression

Mammalian

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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LV-Camk2-Ace-7aa-mScarlet

Plasmid

#129701

Purpose

Expresses Ace-7aa-mScarlet in mammalian cells

Depositor

Yiyang Gong

Article

Beck et al Sci Rep. 2019 Nov 4;9(1):15878. doi: 10.

Insert

Ace-7aa-mScarlet

Use

Lentiviral

Expression

Mammalian

Promoter

Camk2

Available Since

Nov. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 human cofilin S3A

Plasmid

#50854

Purpose

expresses constituvely active cofilin in mammalian cells

Depositor

James Bamburg

Article

Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

Insert

cofilin 1 S3A (CFL1 Human)

Expression

Mammalian

Mutation

constitutively active, S3A

Promoter

CMV

Available Since

Feb. 4, 2014

Availability

Industry, Academic Institutions, and Nonprofits